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Rnf111 (BC069835) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG211379
Rnf111 (tGFP-tagged) - Mouse ring finger 111 (cDNA clone MGC:76599 IMAGE:30094289)
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ORF Plasmid:
tGFP DDK
  $1,098.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rnf111
Synonyms ARK, Arkadia
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG211379 representing BC069835
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTCAATGGACTCCTGAATTTAACGAGCTCTACACCCTAAAAGTGGCTATGAAGAGTGGGACTCCTG
ATGCGCCCACTACACAGGAGAGTCTGAAGGCGGTCCTTCTGCACCCACAGCCCCTAGGGGCCACCAAGAG
CTTCCCTGCAGAAGTGGAGATGATTAATAGCAAAGTGGGGAATGAATTCTCTCATCTGTGTGATGATTCT
CAAAAACAAGAGAAGGACATGACTGGCAACCAGCAAGAACAAGAAAAAAGTGGTGTTGTGAGAAAAAAGC
GCAAAAGCCAGCAGGCTGGCCCCTCATATGTGCAGAACTGTGTCAAAGAAAATCAGGAAATCCTAGGGCG
CAGACAACAGCTAGAGACACCGAGTGATGAGGATAATGACTCTTCCTTAAGTGAGTGTCTGTCTTCTCCC
TCATCTAGTCTCCATTTTGGGGGCTCTGATACAGTGACTTCAGATGAGGATAAAGAAGTCTCGGTGAGAC
ATACCCAGCCTGTTCTGAGTGCTAAAAGTAGAAGTCACAGTGCAAGGTCTCACAAGTGGCCTCGAACTGA
GGCAGATCCTGTGCCAAGTTTGTTAATGAAAAGACCTTGTTTTCATGGCAGTGCACTAAGGAGAGTCACA
TGCAGAAAGAGGCTGGTGAAGAGCAGCTCCTCGCAGAGGACACAGAAGCAGAAGGAGAGGATGCTAGTGC
AGAGGAAGAAGCGGGAGGCGCTAGCTCAGAGGAAGTATGCTCTTCTCTCCAGCTCCAGTAGCTCCAGTGA
GAATGACCTCAGCAGCGATTCCTCGTCCAGCTCCTCCACCGATGGAGAAGAGGACCTGTGTGCATCTGCC
AGTGAGAACCCCAGCAACCCCGCTGCTCCCTCAGGAAGTATTGATGAAGATGTTGTGGTGATAGAAGCCT
CCTTCACTCCCCAGGTCACTGCCAATGAAGAAATTAATGTTACCTCAACTGACAGTGAAGTGGAGATTGT
TACAGTTGGAGAAAGCTATCGGTCTCGTTCAACCCTTGGACACTCCAGATCTCATTGGAGCCAAGGTTCA
AGTTCGCATACAGGACGGCCACAGGAGTCTCGAAACCGCAGTAGGATCTCTACTGTTATACAGCCCCTGA
GGCAGAATGCAGCAGAAGTTGTGGACCTTACTGTTGATGAAGATGAACCTACTATAGTACCAACCACTTC
TGCTAGAATGGACTCACAAACTACTAGTGCTTCCATTAACAATTCAAATCCATCTACCTCTGAGCAAGCC
TCTGATACTACTTCAACTGTGGCCAGTAGCCAGCCTTCCACAGTGTCAGAGACTGAAGCTACTCTTACAA
GCAATAGTGCAACTGGTTCTTCTGTGGGCGATGACGTGAGGAGAACTGCATCTAGTGCTGTCCCAGAAAG
TGGTCCTCCTGCAATGCCAAGGCTACCTTCTTGCTGCCCCCAGCACTCACCATGTGGAGGGACATCACAG
AGCCATCATGCATTAGCACACCCACACTCAAGTTGTTTTCAGCAGCATGGCCACCATTTTCAGCATCACC
ACCATCACCACCATACTCCCCACCCAGCTGTCCCAGTTTCTCCTTCCTTCAGTGACCCTGCTTGCCCAGT
GGAAAGACCTCAAGTACAGGCGCCTTGTGGAGCAAATAGCAGCTCTGGTTCCAGCTACCATGACCAGCAG
GCATTGCCAGTCGATCTGAGCAACAGTGCTCTCAGGACTCATGGAAGTGGGGGTTTCCATGGTGCATCTG
CTTTTGACCCCTGCTGCCCTGTCACATCTTCCCGGGCTGCAGTCTTTGGCCATCAGGCTGCTGCTGCCCC
GACTCAGCCATTATCAATAGATGGCTATGGCTCCAGCATGGTTGCCCAGCCCCAGCCCCAGCCCCCTCCA
CAGCCTTCTCTTTCGTCATGTCGGCACTACATGCCACCTCCGTATGCTTCCTTGACAAGACCACTGCACC
ATCAAGCCTCAGCTTGCCATCACTCTCATGGAAATGCCCCTCCCCAGACTCAGCCTCCCCCTCAAGTGGA
TTACGTGATTCCGCATCCTGTCCATGCTTTCCATTCTCAGATATCTTCTCATGCAGCATCTCACCCTGTG
GCACCACCGCCGCCAACACATTTAGGCAGTACAGCGGCACCCATCCCTCAGCATCTTCCTCCTGCTCACC
AGCCGATTTCACACCATATTCCAGCCCCAGCACCTTCAGCCCAGAGACTGCACCCTCATGAAGTGATGCA
GAGGATGGAAGTTCAGAGGAGGAGGATGATGCAGCATCCAACGCGGGCACATGAACGGCCCCCGCCCCAT
CCACATAGGATGCACCCAAACTATGGCCATGGGCATCATATTCATGTGCCTCAAACCATGTCTTCACATC
CTCGCCAGGCTCCAGAGAGAACTGCCTGGGAACTGGGGATTGAAGCTGGAGTAACTGCAGCTACCTACAC
ACCTGGTGCTCTGCACCCTCACCTGGCCCATTACCACGCCCCGCCTCGACTTCATCACCTCCAGTTAGGA
GCACTTCCTTTAATGGTTCCTGACATGGCAGGCTATCCTCACATCCGTTATATTTCTTCAGGATTGGATG
GAGCATCATTCAGAGGTCCTTTTAGGGGCAATTTTGAGGAATTGATTCATCTGGAAGAAAGATTAGGAAA
TGTCAATCGTGGAGCCTCCCAGGGAACAATTGAAAGATGCACATATCCACATAAATATAAAAAGAGGAAA
CTGCACTGCAAACAAGATGGGGAAGAAGGGACTGAGGAAGACACAGAGGAAAAATGTACTATCTGTTTGT
CTATTTTAGAGGAAGGTGAAGATGTGAGGCGCCTTCCATGTATGCACCTTTTCCACCAAGTGTGTGTTGA
TCAGTGGCTGATCACCAATAAGAAGTGCCCCATATGCAGAGTGGACATCGAGGCCCAGCTGCCAAGTGAA
AGT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG211379 representing BC069835
Red=Cloning site Green=Tags(s)

MSQWTPEFNELYTLKVAMKSGTPDAPTTQESLKAVLLHPQPLGATKSFPAEVEMINSKVGNEFSHLCDDS
QKQEKDMTGNQQEQEKSGVVRKKRKSQQAGPSYVQNCVKENQEILGRRQQLETPSDEDNDSSLSECLSSP
SSSLHFGGSDTVTSDEDKEVSVRHTQPVLSAKSRSHSARSHKWPRTEADPVPSLLMKRPCFHGSALRRVT
CRKRLVKSSSSQRTQKQKERMLVQRKKREALAQRKYALLSSSSSSSENDLSSDSSSSSSTDGEEDLCASA
SENPSNPAAPSGSIDEDVVVIEASFTPQVTANEEINVTSTDSEVEIVTVGESYRSRSTLGHSRSHWSQGS
SSHTGRPQESRNRSRISTVIQPLRQNAAEVVDLTVDEDEPTIVPTTSARMDSQTTSASINNSNPSTSEQA
SDTTSTVASSQPSTVSETEATLTSNSATGSSVGDDVRRTASSAVPESGPPAMPRLPSCCPQHSPCGGTSQ
SHHALAHPHSSCFQQHGHHFQHHHHHHHTPHPAVPVSPSFSDPACPVERPQVQAPCGANSSSGSSYHDQQ
ALPVDLSNSALRTHGSGGFHGASAFDPCCPVTSSRAAVFGHQAAAAPTQPLSIDGYGSSMVAQPQPQPPP
QPSLSSCRHYMPPPYASLTRPLHHQASACHHSHGNAPPQTQPPPQVDYVIPHPVHAFHSQISSHAASHPV
APPPPTHLGSTAAPIPQHLPPAHQPISHHIPAPAPSAQRLHPHEVMQRMEVQRRRMMQHPTRAHERPPPH
PHRMHPNYGHGHHIHVPQTMSSHPRQAPERTAWELGIEAGVTAATYTPGALHPHLAHYHAPPRLHHLQLG
ALPLMVPDMAGYPHIRYISSGLDGASFRGPFRGNFEELIHLEERLGNVNRGASQGTIERCTYPHKYKKRK
LHCKQDGEEGTEEDTEEKCTICLSILEEGEDVRRLPCMHLFHQVCVDQWLITNKKCPICRVDIEAQLPSE
S

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC069835
ORF Size 2943 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC069835, AAH69835
RefSeq Size 4725 bp
RefSeq ORF 2945 bp
Locus ID 93836
Cytogenetics 9 39.53 cM
Summary E3 ubiquitin-protein ligase required for mesoderm patterning during embryonic development (PubMed:11298452). Acts as an enhancer of the transcriptional responses of the SMAD2/SMAD3 effectors, which are activated downstream of BMP (PubMed:14657019). Acts by mediating ubiquitination and degradation of SMAD inhibitors such as SMAD7, inducing their proteasomal degradation and thereby enhancing the transcriptional activity of TGF-beta and BMP (PubMed:14657019). In addition to enhance transcription of SMAD2/SMAD3 effectors, also regulates their turnover by mediating their ubiquitination and subsequent degradation, coupling their activation with degradation, thereby ensuring that only effectors 'in use' are degraded (By similarity). Activates SMAD3/SMAD4-dependent transcription by triggering signal-induced degradation of SNON isoform of SKIL (By similarity). Associates with UBE2D2 as an E2 enzyme (By similarity). Specifically binds polysumoylated chains via SUMO interaction motifs (SIMs) and mediates ubiquitination of sumoylated substrates (PubMed:23530056). Catalyzes 'Lys-63'-linked ubiquitination of sumoylated XPC in response to UV irradiation, promoting nucleotide excision repair (By similarity). Mediates ubiquitination and degradation of sumoylated PML (PubMed:23530056). The regulation of the BMP-SMAD signaling is however independent of sumoylation and is not dependent of SUMO interaction motifs (SIMs) (PubMed:23530056).UniProtKB/Swiss-Prot Function
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"BC069835" in other vectors (4)
SKU Description Size Price
MC220666 Rnf111 (untagged) - Mouse ring finger 111 (cDNA clone MGC:76599 IMAGE:30094289), (10ug) 10 ug
$989.00
MR211379 Rnf111 (Myc-DDK-tagged) - Mouse ring finger 111 (cDNA clone MGC:76599 IMAGE:30094289) 10 ug
$589.00 $898.00
MR211379L3 Lenti ORF clone of Rnf111 (Myc-DDK-tagged) - Mouse ring finger 111 (cDNA clone MGC:76599 IMAGE:30094289) 10 ug
$1,198.00
MR211379L4 Lenti ORF clone of Rnf111 (mGFP-tagged) - Mouse ring finger 111 (cDNA clone MGC:76599 IMAGE:30094289) 10 ug
$1,198.00

Plasmid Map for MG211379

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG211379
Circular map for MG211379

file-alt Citations

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