Limch1 (BC075634) Mouse Tagged ORF Clone

SKU MG211106

Limch1 (tGFP-tagged) - Mouse LIM and calponin homology domains 1 (cDNA clone MGC:100029 IMAGE:30544497)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Limch1
• Synonyms: mKIAA1102
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211106 representing BC075634; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCACAAAACATGTACAATACGGTCCTTCATACGCGACTTTTATAGAATCCTTTTTACATGCGCAAGGC; CATCCATTATGTCAGATGACGCAGAGAGCACGAGTATGTTTGACATGCGGTGTGAGGAGGAGGCAGCGGT; GCTGCCGCACAGCAGGGCCCGCCAGGAGCAGCTGCAGCCCGAACCACCTCCGGAGCCTGCCTTCGCCGAG; GCACAGAAGTGGATCGAGCAAGTCACTGGTCGAAGTTTTGGTGACAAAGACTTCCGGACAGGACTAGAAA; ATGGGATCCTCCTTTGCGAGTTGCTGAATGCTATAAAGCCAGGACTTGTTAAAAAGATCAATAGATTGCC; TACCCCCATTGCAGGATTGGACAATACCATCTTATTCTTGAGAGGCTGTAAGGAGCTTGGCCTTAAAGAA; TCTCAACTCTTTGACCCGAGTGACCTCCAGGATACCTCCAACAGGGTCACTGTCAAGAACCTCGATTATA; GCAGGAAGCTGAAAAACGTGCTAGTGACCATTTACTGGCTGGGGAAAGCTGCAAACAGCTGTGCATCCTA; CGGTGGAACCACACTGAACCTGAAGGAGTTTGAAGGGCTGTTGGCTCAGATGCGAAAGGAGACTGACGAC; ATTGATAGCCCGAAGCGCAGCATCCGAGACAGTGGCTACATCGACTGCTGGGACTCTGAACGCAGCGACT; CCCTCTCTCCTCCTCGCCACGGACGGGAAGATGGGACAAGTGAGCGGAGGAAAAGCATCAAAACTTACAG; AGAAATCGTCCAAGAAAAAGAGCGGAGAGAGAGGGAACTTCATGAGGCGTACAAGAACGCAAGGTCGCAG; GAGGAGGCCGAGGGGATCCTCCAGCAGTACATCGAGAGGTTCACCATCAGTGAGGCTGTCCTCGAACGCT; TGGAGATGCCAAAAATCCTGGAAAGAAGCCATTCAACAGAGCCAAACGTGTCCTCCTTCCCAAACGACCC; CAGCCCCATGAAGTACCTGCGGCAGCAGTCACTGCCCCCACCCAAGTTCACTGCCACGGTTGAGACCACC; ATAGCTCGCACCAGTGTCCCAGAGAGCATTGCGTCAGCAGGCACTGGGTCTCCAAGCAAAATCATCACTC; CAAACACAGTGCCTATGCTGACACCCAGGCCTTACTCCCAGCCCAAAAACTCTCAAGAGGTTCTGAAGAC; TTTTAAGGTAGATGGGAAGGTCAGCATGAATGGGGAAATGGCCCCTGGAGATGAGGAAGGAAAGGAAAAA; GAAGGCCCTGCAGCTGCAGCCCCTGGCCCTTCCTTAACCAAGTCCCAGATGTTTGAAGGTGTGGCCACGG; TGCACGACTCTCCTGTGCAGGTGAAACAAGGCAGCAACAGCATCGAGATCAACATCAAGAAGCCAAATTC; TGCTCCCCAGGAACTGACAGCAGCCTCTGAGGAAACTGAATCGAACGGCCAAGAGGATGAAGACGGTGAA; GAAAGGCCAGGGACAGGGGATTTGGAGCCTGACTCGGCAGAACCCCAGCACTTTACGACAACTGTGACTC; GGTGCAGCCCAACAGTGGCCTTGGTGGAGTTTTCACCCAACCCGCAGCTGAAGAATGAGGTGCCAGAACA; AGGCCAGAAGAAGCCAGAAGATGAGATGAGTGGGAAGGTGGAGTTAGTGTTGTCACAGAAGGTGGCAAAG; CCAAAATCCCCAGAGCCTGAAGCAACCCTGACGTTTCCATTTCTCGACAAAATGCCTGAAACCAACCAAC; TACATTTGCCAAATCCCAGTTCTCAAGCGGATTCTCCAAGCAGTGAAAAGTCCCCTGGGAGCACACCCTT; TAAGTTCTGGGCATGGGACCCAGAAGAGGAGCGCCGGCGACAGGAGAAATGGCAGCAGGAGCAGGAACGT; CTGCTGCAGGAGAGGTATCAGAAGGAGCAAGACAAGCTGAAGGAGGAGTGGGAAAAGGCCCAGAAGGAGG; TCGAAGAAGAAGAACGCAGATACTACGAGGAGGAGCGTAAGATAATTGAAGACACGGTGGTTCCATTCAC; TATTTCCTCAAGCTCTGCAGACCAACTGTCTACGTCCTTGTCTGTAACTGAAGGCAGCGGGACAAGGAAT; AAGATGGACTTGGAAAACTGCCCAGACAAAGAAAACGAGAGAAGACAGAAGACGCCTTTCCAGGAGAATG; ACGGTGATTCATTGCTCAAAACTAGAGAAGGTGGTCTGCCAGAGGAGCAGAGCCTAACTCCAAGTCCCTC; GGCTAACCCTGAAATCTCCGTGTCAAAAGGAATCCATCAGGACCCGCAGCTGGAGGCAGAGGCTGGGGCC; CCACACTGTGGTACAAACCCACAGCCAGCTCAGGATCCACCTCGGAATCAACAGATACCAAACCCACCAA; CATCCACGTCGGAAGATGTGAAGCCCAAAACCTTGGCCCTGGAGAAAACCATCAATCATCAGATGGAGTC; TCCGGGGGAAAGGCGGAAGTCTATAAGCGGGAAGAAACTGTGCTCCTCCTGCGGGCTCACTTTGGGTAAA; GGTGCTGCGATGATCATCGAGACCCTGAATCTCTACTTTCACATTCAGTGTTTCAGGTGCGGCATCTGTA; AAGGACAGCTCGGAGATGCAGTAAGCGGGACAGACGTCAGGATTCGCAATGGTCTCCTAAACTGTACCGA; CTGCTACATGCGATCCAGAAGTGCCGGCCAGCCTACAACACTG; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211106 representing BC075634; Red=Cloning site Green=Tags(s); ; MHKTCTIRSFIRDFYRILFTCARPSIMSDDAESTSMFDMRCEEEAAVLPHSRARQEQLQPEPPPEPAFAE; AQKWIEQVTGRSFGDKDFRTGLENGILLCELLNAIKPGLVKKINRLPTPIAGLDNTILFLRGCKELGLKE; SQLFDPSDLQDTSNRVTVKNLDYSRKLKNVLVTIYWLGKAANSCASYGGTTLNLKEFEGLLAQMRKETDD; IDSPKRSIRDSGYIDCWDSERSDSLSPPRHGREDGTSERRKSIKTYREIVQEKERRERELHEAYKNARSQ; EEAEGILQQYIERFTISEAVLERLEMPKILERSHSTEPNVSSFPNDPSPMKYLRQQSLPPPKFTATVETT; IARTSVPESIASAGTGSPSKIITPNTVPMLTPRPYSQPKNSQEVLKTFKVDGKVSMNGEMAPGDEEGKEK; EGPAAAAPGPSLTKSQMFEGVATVHDSPVQVKQGSNSIEINIKKPNSAPQELTAASEETESNGQEDEDGE; ERPGTGDLEPDSAEPQHFTTTVTRCSPTVALVEFSPNPQLKNEVPEQGQKKPEDEMSGKVELVLSQKVAK; PKSPEPEATLTFPFLDKMPETNQLHLPNPSSQADSPSSEKSPGSTPFKFWAWDPEEERRRQEKWQQEQER; LLQERYQKEQDKLKEEWEKAQKEVEEEERRYYEEERKIIEDTVVPFTISSSSADQLSTSLSVTEGSGTRN; KMDLENCPDKENERRQKTPFQENDGDSLLKTREGGLPEEQSLTPSPSANPEISVSKGIHQDPQLEAEAGA; PHCGTNPQPAQDPPRNQQIPNPPTSTSEDVKPKTLALEKTINHQMESPGERRKSISGKKLCSSCGLTLGK; GAAMIIETLNLYFHIQCFRCGICKGQLGDAVSGTDVRIRNGLLNCTDCYMRSRSAGQPTTL; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC075634
• ORF Size: 2703 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC075634
• RefSeq Size: 4698 bp
• RefSeq ORF: 2705 bp
• Locus ID: 77569
• Cytogenetics: 5 C3.1
• Summary: Actin stress fibers-associated protein that activates non-muscle myosin IIa. Activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. Through the activation of non-muscle myosin IIa, positively regulates actin stress fibers assembly and stabilizes focal adhesions. It therefore negatively regulates cell spreading and cell migration.[UniProtKB/Swiss-Prot Function]