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C2cd5 (NM_029081) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG210801
C2cd5 (tGFP-tagged) - Mouse RIKEN cDNA 5730419I09 gene (5730419I09Rik)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $950.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol C2cd5
Synonyms 5730419I09Rik; C030008B15Rik; CDP138
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG210801 representing NM_029081
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCAGGTGAATTACAGAGAAAGATTGGCTTGAAAGTCCTCGAGATGAGAGGGAATGCAGTTGTTGGTT
ACTTGCAGTGCTTTGATCTGGAGGGCGAGTCTGGGTTAGTGGTCCGAGCCATAGGAACGGCGTGCACTCT
GGATAAACTGAGCAGCCCAGCAGCGTTCCTTCCTGCATGCAGCTCCCCATCCAGAGAACTGAAGGAGATT
CCCTTCAATGAGGATCCCAACCCCAATACTCACTCATCAGGACCCTCAACCCCTCTGAAAAACCAAACTT
ATTCCTTTTCACCTTCCAAGTCCTACAGTCGGCAGTCCTCTTCTTCTGACACAGATTTGAGTTTGACACC
CAAGACTGGAATGGGCAGTGGGAGTGCTGGAAAAGAAGGGGGACCCTTTAAAGCCCTTTTACGGCAACAG
ACGCAGTCGGCGTTGGAACAGCGGGAGTTCCCGTTCCTCACCCTGACGGCCTTCCCCCCTGGACTTCTTG
TGCACGTCGGTGGCGTTGTCAGTGCACGCTCTGTGAAGCTTTTAGATCGGATCCACAACCCTGATGAACC
AGAAACTCGAGATGCATGGTGGGCAGAAATCAGACAAGAGATAAAGTCACATGCTAAAGCCTTAGGCTGC
CATGCTGTAGTGGGCTACAGCGAGTCAACCAGCATCTGTGAAGAAGTCTGTATTTTATCAGCGTCTGGCA
CGGCAGCTGTGCTGAACCCTCGGTTTCTGCAGGAAGGCACTGTGGAGGGCTGTTTGGAACAAAGGATTGA
AGAGAATTTACCGGTGGGCTGCGGATTCTGCCATATACCATATGATGAACTGAACATGCCGTTTCCCGCT
CATCTCACATATTGCTATAACTGCAGGAAGCAAAAAGTCCCTGATGTTCTCTTCACAACTATCGATCTGC
CCACAGACGCAGTGGTTGTTGGTAAAGGTTGTCTTATTCAGGCAAGGTTATGTCGATTAAAAAAGAAAGC
ACAGGCAGAAGCCAATGCCACAGCTATCAGTAATCTCTTGCCGTTTATGGAATATGAAGTACACACTCAA
CTGATGAACAAGCTCAAACTCAAAGGAATGAATGCTTTGTTTGGACTGAGAATTCAGATCACAGTGGGCG
AGACCATGCTGATGGGCTTAGCGTCGGCCACAGGAGTCTACTTAGCAGCCTTACCAACACCCGGTGGAAT
CCAGATTGCTGGGAAGACCCCTAATGATGGCTCCTATGAACAGCATATCTCCCACATGCAGAAGAGAATA
AACGACACCATTGCAAAAAACAAAGAACTGTATGAGATCAGCCCCCCGGAGGTGTCTGAGGAGATGATAG
GATCCCCCATCCCAGAACCCAGACAGCGCTCCAGACTTCTAAGATCACAGTCGGAAAGTTCTGATGAAGT
TACAGAGTTAGATCTTTCACACGGGAAAAAGGATGCTTTTGTTTTAGAGATCGATGACACCGATGCCATG
GAAGACGTGCACTCCCTCCTTACTGACGCCCCACCCCCTTCAGGTTTTTATAGTTGTAATACAGAAATTA
TGCCTGGCATAAATAACTGGACATCAGAAATACAGATGTTCACGTCAGTAAGAGTGGTCAGACTAAGTAG
CCTCAACTTGACTAACCAAGCTCTCAATAAGAACTTCAACGGCCTATGTGAGAACTTGCTCAAGAGCCTG
TATTTCAAACTTCGGTCCATGACCCCCTGCTGCCTCTGCCATGTGAACTTTACAGTGTCTCTGCCTGAAG
ATGAGTTAATCCAGGTCACAGTCACAGCAGTTGCAATAACTTTTGATAAAAACCAGGCCTTACAGACCAC
AAAACCCCACGTTGAAAAGTCACTACAAAGAGCCTCCACAGATAACGAAGAACTGCTGCAGTTCCCACTG
GAGCTCTGCTCAGACTCACTGCCTCCTCATCCTTTCCCAGCAGCTAAAGAACACCTGGAGAGTGCGAACT
CTAACTCAGGTATACCAGCTGCACAGAGAGCTGTGACTGTTGAAAAAGCAAGTGCCATGGGTGATGGAAA
TTTCCGGAATCGCTCTGCTCCACCTTGCGCAAGTCCCACAGTTGGGGTTGTTAAGATGACGCCGCTTTCT
TTCATCCCTGGAGCAAAGATAACCAAGTACCTCGGCATCATTAACATGTTTTTCATTCGGGAAACCACTT
CTCTCCGCGAGGAAGGCGGAGTCAGTGGTTTTCTCCACGCTTTCATTGCTGAAGTGTTTGCCATGGTCCG
AGCTCACGTGGCAGCGCTGGGCGGGAATGCTGTTGTGTCCTACATCATGAAGCAGTGTGTCTTCATGGAG
AACCCCAGTAAGAACCAGGCACAGTGTCTCATAAATGTCAGTGGGGACGCGGTGGTGTTTGTTCGTGACT
CCGACTTAGAGGTCATGTCATCTCAGCAGCCTGCTGCCAACTGCCAGCCCTCATGTACCGGAGAAGTTAC
AACT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG210801 representing NM_029081
Red=Cloning site Green=Tags(s)

MSGELQRKIGLKVLEMRGNAVVGYLQCFDLEGESGLVVRAIGTACTLDKLSSPAAFLPACSSPSRELKEI
PFNEDPNPNTHSSGPSTPLKNQTYSFSPSKSYSRQSSSSDTDLSLTPKTGMGSGSAGKEGGPFKALLRQQ
TQSALEQREFPFLTLTAFPPGLLVHVGGVVSARSVKLLDRIHNPDEPETRDAWWAEIRQEIKSHAKALGC
HAVVGYSESTSICEEVCILSASGTAAVLNPRFLQEGTVEGCLEQRIEENLPVGCGFCHIPYDELNMPFPA
HLTYCYNCRKQKVPDVLFTTIDLPTDAVVVGKGCLIQARLCRLKKKAQAEANATAISNLLPFMEYEVHTQ
LMNKLKLKGMNALFGLRIQITVGETMLMGLASATGVYLAALPTPGGIQIAGKTPNDGSYEQHISHMQKRI
NDTIAKNKELYEISPPEVSEEMIGSPIPEPRQRSRLLRSQSESSDEVTELDLSHGKKDAFVLEIDDTDAM
EDVHSLLTDAPPPSGFYSCNTEIMPGINNWTSEIQMFTSVRVVRLSSLNLTNQALNKNFNGLCENLLKSL
YFKLRSMTPCCLCHVNFTVSLPEDELIQVTVTAVAITFDKNQALQTTKPHVEKSLQRASTDNEELLQFPL
ELCSDSLPPHPFPAAKEHLESANSNSGIPAAQRAVTVEKASAMGDGNFRNRSAPPCASPTVGVVKMTPLS
FIPGAKITKYLGIINMFFIRETTSLREEGGVSGFLHAFIAEVFAMVRAHVAALGGNAVVSYIMKQCVFME
NPSKNQAQCLINVSGDAVVFVRDSDLEVMSSQQPAANCQPSCTGEVTT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_029081
ORF Size 2454 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_029081.1, NP_083357.1
RefSeq Size 4202 bp
RefSeq ORF 3051 bp
Locus ID 74741
UniProt ID Q7TPS5
Cytogenetics 6 G3
Summary Required for insulin-stimulated glucose transport and glucose transporter SLC2A4/GLUT4 translocation from intracellular glucose storage vesicle (GSV) to the plasma membrane (PM) in adipocytes. Binds phospholipid membranes in a calcium-dependent manner and is necessary for the optimal membrane fusion between SLC2A4/GLUT4 GSV and the PM.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_029081" in other vectors (4)
SKU Description Size Price
MC204482 C2cd5 (untagged) - Mouse RIKEN cDNA 5730419I09 gene (5730419I09Rik), transcript variant 1, (10ug) 10 ug
$750.00
MR210801 C2cd5 (Myc-DDK-tagged) - Mouse RIKEN cDNA 5730419I09 gene (5730419I09Rik), transcript variant 1 10 ug
$589.00 $750.00
MR210801L3 Lenti ORF clone of C2cd5 (Myc-DDK-tagged) - Mouse RIKEN cDNA 5730419I09 gene (5730419I09Rik), transcript variant 1 10 ug
$1,050.00
MR210801L4 Lenti ORF clone of C2cd5 (mGFP-tagged) - Mouse RIKEN cDNA 5730419I09 gene (5730419I09Rik), transcript variant 1 10 ug
$1,050.00

Plasmid Map for MG210801

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG210801
Circular map for MG210801

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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