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Trpc4ap (BC033274) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG210709
Trpc4ap (tGFP-tagged) - Mouse transient receptor potential cation channel, subfamily C, member 4 associated protein (cDNA clone MGC:25542
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $930.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Trpc4ap
Synonyms truss, mFLJ00177
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG210709 representing BC033274
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCAGCGCCCGCAGCGGCTGGAGCTGGGGCCAGCCGGGGGAGGCGGTTGGCAGCTACCGCGGCGG
CGTGGGGCGGCTGGGGAGGTCGACCGCGGCCGGGTAACATCCTGCTGCAGCTGCGGCAGGGCCAGCTCAC
CGGCCGAGGCCTGGTCCGGGCGGTGCAGTTCACTGAGACCTTTTTGACCGAGAGGGACAAATTGTCCAAA
TGGAGTGGAATTCCTCAGTTGCTCCTTAAGCTGTATGCCACCAGCCATCTGCACAGTGACTTCGTTGAGT
GCCAAAGCATTCTCAAGGAGATTTCTCCTCTTCTCTCTATGGAGGCTATGGCGTTTGTAACTGAAGACAG
GAAATTTACTCAAGAAGCTACATACCCAAATACATATATTTTTGACTTATTTGGAGGTGTTGATCTTCTT
GTAGAAATTCTTATGAGGCCTACCATCTCTATCCGGGGACAGAAACTAAAAATAAGTGACGAGATGTCGA
AGGACTGCTTGAGCATCCTGTACAATACCTGTGTCTGCACGGAAGGAGTAACGAAGCGTTTGGCAGAAAA
GAATGATTTTGTGATTTTTCTGTTCACACTGATGACAAGTAAGAAGACATTCTTACAAACAGCGACTCTC
ATTGAAGACATTTTGGGTGTTAAGAAGGAAATGATCCGGCTAGATGAAGTTCCCAATCTGAGTTCCTTAG
TATCCAATTTCGATCAGCAGCAGCTTGCTAACTTCTGCAGGATCCTGGCTGTCACCATTTCAGAGATGGA
CACGGGGAATGACGACAAACACACGCTTCTTGCCAAAAATGCTCAGCAGAAGAAGAGTTTGAGTTTGGGG
CCTTCTGCAGCTGAAATCAACCAAGCTGCTCTTCTCAGCATCCCTGGCTTTGTGGAACGGCTTTGCAAAC
TGGCAACTAGGAAAGTGTCAGAGTCAACGGGCACAGCCAGCTTCCTTCAAGAACTGGAGGAGTGGTACAC
ATGGCTCGACAATGCGTTGGTGCTAGATGCCCTGATGCGAGTGGCCAATGAGGAGTCTGAACACAATCAA
GCCCCCACTGTGTTCCCTTCTCTAGGCACTTCGGAGGAGGGTGGGCTGCCACACACCTCGGCCAGGGCGC
AGCTGCCCCAGTCCATGAAGATTATGCATGAGATTATGTACAAACTGGAAGTGCTTTATGTCCTCTGCGT
GCTGCTGATGGGGCGTCAGCGAAACCAGGTCCACAGGATGATTGCTGAGTTCAAGTTGATCCCAGGACTC
AACAATTTGTTCGACAAACTGATTTGGAGGAAGCATTCAGCATCTGCCCTTGTCCTCCATGGCCACAACC
AGAACTGTGACTGTAGCCCGGACATCACCTTGAAGATCCAGTTTTTGAGACTTCTCCAGAGTTTCAGTGA
CCACCATGAGAACAAGTACTTGCTGCTCAACAACCAGGAGCTAAATGAGCTCAGTGCCATCTCGCTGAAA
GCCAACATCCCTGAGGTGGAGGCTGTCCTCAACACTGACAGGAGTCTGGTGTGTGACGGCAAGAGGGGCC
TACTAACTCGGCTGCTGCAGGTCATGAAGAAGGAGCCAGCGGAGTCTTCATTCAGGTTTTGGCAAGCCCG
GGCTGTGGAAAGTTTCCTCCGAGGGACCACCTCCTATGCAGACCAGATGTTCCTGCTGAAGCGAGGCCTT
CTGGAGCACATCTTATACTGCATTGTGGACAGTGAGTGCAAGTCTAGGGATGTACTCCAGAGCTACTTTG
ATCTCCTGGGAGAGCTGATGAAGTTCAACGTCGATGCGTTCAAGAGATTCAATAAATATATCAACACTGA
TGCAAAGTTCCAGGTGTTCCTGAAACAGATCAACAGCTCGCTAGTGGACTCCAACATGCTGGTGCGCTGT
GTCACTCTGTCACTGGACCGATTTGAAAACCAGGTGGACATGAAAGTGGCCGAGGTCCTGTCCGAGTGCC
GCCTGCTTGCCTACATATCCCAGGTGCCCACGCAAATGTCCTTCCTCTTCCGCCTCATCAATATCATCCA
TGTGCAGACGCTAACCCAGGAGAACGTCAGCTGCCTCAACACCAGCCTGGTAATCCTGATGCTGGCCCGA
CGGAAAGAGCGGCTGCCCCTGTACCTGCGACTGCTACAGCGAATGGAGCACAGCAAGAAATACCCCGGCT
TCCTGCTCAACAACTTCCACAACCTGCTGCGCTTTTGGCAGCAGCACTACCTGCACAAGGATAAGGACAG
CACCTGCCTGGAGAATAGCTCCTGTATCAGCTTCTCGTACTGGAAGGAGACAGTGTCCATCTTGTTGAAC
CCAGACCGCCAGTCGCCCTCTGCCCTCGTCAGCTACATTGAGGAGCCCTACATGGACATAGACAGAGACT
TCACTGAGGAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG210709 representing BC033274
Red=Cloning site Green=Tags(s)

MAAAPAAAGAGASRGRRLAATAAAWGGWGGRPRPGNILLQLRQGQLTGRGLVRAVQFTETFLTERDKLSK
WSGIPQLLLKLYATSHLHSDFVECQSILKEISPLLSMEAMAFVTEDRKFTQEATYPNTYIFDLFGGVDLL
VEILMRPTISIRGQKLKISDEMSKDCLSILYNTCVCTEGVTKRLAEKNDFVIFLFTLMTSKKTFLQTATL
IEDILGVKKEMIRLDEVPNLSSLVSNFDQQQLANFCRILAVTISEMDTGNDDKHTLLAKNAQQKKSLSLG
PSAAEINQAALLSIPGFVERLCKLATRKVSESTGTASFLQELEEWYTWLDNALVLDALMRVANEESEHNQ
APTVFPSLGTSEEGGLPHTSARAQLPQSMKIMHEIMYKLEVLYVLCVLLMGRQRNQVHRMIAEFKLIPGL
NNLFDKLIWRKHSASALVLHGHNQNCDCSPDITLKIQFLRLLQSFSDHHENKYLLLNNQELNELSAISLK
ANIPEVEAVLNTDRSLVCDGKRGLLTRLLQVMKKEPAESSFRFWQARAVESFLRGTTSYADQMFLLKRGL
LEHILYCIVDSECKSRDVLQSYFDLLGELMKFNVDAFKRFNKYINTDAKFQVFLKQINSSLVDSNMLVRC
VTLSLDRFENQVDMKVAEVLSECRLLAYISQVPTQMSFLFRLINIIHVQTLTQENVSCLNTSLVILMLAR
RKERLPLYLRLLQRMEHSKKYPGFLLNNFHNLLRFWQQHYLHKDKDSTCLENSSCISFSYWKETVSILLN
PDRQSPSALVSYIEEPYMDIDRDFTEE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC033274
ORF Size 2391 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC033274.1
RefSeq Size 3133 bp
RefSeq ORF 2393 bp
Locus ID 56407
Cytogenetics 2 77.26 cM
Summary Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. The DCX(TRUSS) complex specifically mediates the polyubiquitination and subsequent degradation of MYC (By similarity). Also participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. Involved in JNK activation via its interaction with TRAF2. Also involved in elevation of endoplasmic reticulum Ca(2+) storage reduction in response to CHRM1.UniProtKB/Swiss-Prot Function
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Resources
"BC033274" in other vectors (4)
SKU Description Size Price
MC220798 Trpc4ap (untagged) - Mouse transient receptor potential cation channel, subfamily C, member 4 associated protein (cDNA clone MGC:25542, (10ug) 10 ug
$804.00
MR210709 Trpc4ap (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily C, member 4 associated protein (cDNA clone MGC:25542 10 ug
$589.00 $730.00
MR210709L3 Lenti ORF clone of Trpc4ap (Myc-DDK-tagged) - Mouse transient receptor potential cation channel, subfamily C, member 4 associated protein (cDNA clone MGC:25542 10 ug
$1,030.00
MR210709L4 Lenti ORF clone of Trpc4ap (mGFP-tagged) - Mouse transient receptor potential cation channel, subfamily C, member 4 associated protein (cDNA clone MGC:25542 10 ug
$1,030.00

Plasmid Map for MG210709

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG210709
Circular map for MG210709

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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