Ercc3 (NM_133658) Mouse Tagged ORF Clone

SKU MG210632

Ercc3 (tGFP-tagged) - Mouse excision repair cross-complementing rodent repair deficiency, complementation group 3 (Ercc3)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Ercc3
• Synonyms: BTF2 p89; Ercc-3; XPB
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210632 representing NM_133658; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGGCAAAAGAGATCGAGTGGACCGCGACAAAAAGAAATCCAAGAAGAGGCAGTATGAAGAGGAAGAGG; AAGACGAAGACGACATTCCTGGGAACGAGTCTCAGGAGGCGGTGCCCTCCGCCGCTGGGAAACAGGTGGA; CGAGTCCAGCACCAAAGTGGATGAGTATGGAGCAAAGGACTACAGACAGCAGATGCCACTAAAGGGTGAC; CATACCTCCAGGCCCCTCTGGGTGGCTCCTGATGGCCACATTTTCTTGGAAGCCTTCTCTCCAGTTTACA; AATATGCCCAAGACTTCCTGGTGGCAATTGCAGAGCCGGTGTGCCGGCCTACTCATGTACATGAATACAA; GCTAACCGCCTACTCCCTCTATGCAGCGGTCAGTGTTGGGCTGCAAACCAGTGACATTACTGAGTACCTC; AGAAAGCTCAGTAAGACTGGAGTTCCTGATGGAATTATCCAGTTTATTAAGCTGTGCACTGTCAGCTATG; GGAAGGTCAAGCTGGTCCTCAAGCACAACAGGTACTTTGTTGAAAGTTCCCACCCTGATGTTATCCAGCA; TCTTCTTCAAGACCCAGTGATCCGGGAATGTCGCTTGAGGAACGCCGAGGGCGAGGCCACCGAACTCATC; ACAGAGACTTTTACAAGCAAATCTGCTATTTCTAAGACGGCGGCGGAAGGCAGTGGCGGGCCTTCTACTT; CGCAGGGGGTAGATGCACAGGCCACGTCTGACATACCCAAAGACCTGTTTGATTTTTATGAGCAAATGGA; CAAGGATGAGGAGGAGGAGGAAGAGACACAGACAGTGTCCTTTGAAGTTAAGCAGGAAATGATCGAGGAG; CTGCAGAAGCGCTGCATCTGCTTAGAGTACCCGCTGCTGGCAGAGTATGACTTCCGGAATGACACTCTCA; ATCCTGACATCAACATTGACCTGAAGCCCACAGCCGTACTCAGACCCTATCAGGAGAAGAGCCTGCGGAA; GATGTTTGGGAACGGACGAGCACGCTCAGGAGTCATTGTTCTTCCCTGTGGTGCTGGGAAGTCCCTGGTC; GGCGTGACTGCCGCATGCACTGTCAGAAAGCGCTGTCTCGTCCTGGGCAACTCGGCTGTGTCTGTGGAGC; AGTGGAAAGCCCAGTTTAAGATGTGGTCAACCATCGATGACAGCCAGATCTGCCGCTTCACCTCAGATGC; CAAGGACAAGCCCATCGGCTGCTCCGTTGCCATTAGCACTTACTCTATGCTGGGCCACACCACCAAAAGG; TCCTGGGAAGCTGAGAGAGTCATGGAGTGGCTCAAAACCCAGGAGTGGGGCCTCATGATCCTTGACGAGG; TGCACACCATTCCAGCCAGGATGTTCCGGCGGGTTCTGACTATTGTGCAGGCGCACTGTAAGCTTGGTTT; GACTGCAACCCTTGTCCGGGAAGATGACAAAATTGTTGACTTAAATTTCCTGATTGGGCCCAAGCTCTAT; GAAGCCAACTGGATGGAGCTGCAGAACAATGGGTACATCGCTAAAGTCCAGTGTGCTGAGGTTTGGTGCC; CGATGTCTCCCGAGTTCTACCGAGAGTATGTGGCAATCAAAACAAAGAAACGGATCCTGTTGTACACCAT; GAATCCCAACAAATTCAGAGCCTGCCAGTTTCTGATCAAGTTTCATGAAAGGAGGAATGACAAGATTATT; GTCTTTGCTGACAACGTATTTGCCTTGAAGGAATATGCCATTCGGCTGAACAAACCTTATATCTACGGGC; CCACGTCCCAGGGAGAACGTATGCAGATTCTCCAGAACTTCAAGCACAACCCCAAAATCAACACCATCTT; CATCTCCAAGGTTGGTGACACATCCTTTGATCTGCCAGAAGCCAATGTCCTCATTCAGATCTCTTCCCAT; GGTGGCTCCCGACGGCAGGAAGCCCAGAGACTGGGACGGGTACTCAGAGCCAAGAAAGGGATGGTCGCAG; AGGAATACAATGCCTTTTTCTACTCCCTGGTATCTCAGGACACACAGGAAATGGCTTATTCTACCAAGCG; ACAGAGATTCCTAGTGGATCAAGGTTACAGCTTTAAGGTAATCACAAAGCTAGCTGGCATGGAGGAAGAG; GAGCTGGCGTTCTCCACCAAAGAGGAGCAGCAGCAGCTCCTGCAGAAGGTGCTGGCAGCCACCGACTTGG; ATGCAGAGGAGGAAGTGGTGGCTGGAGAGTTTGGCTCTAGATCTGGCCAGGCATCCCGACGCTGTGGCAC; CATGAGCTCCCTGTCAGGCGCAGACGACACCGTGTACATGGAGTACCACTCCTCCCGAAGCAAGGCCTCC; AGCAAGCACGTGCACCCGCTTTTCAAACGCTTCAGGAAG; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210632 representing NM_133658; Red=Cloning site Green=Tags(s); ; MGKRDRVDRDKKKSKKRQYEEEEEDEDDIPGNESQEAVPSAAGKQVDESSTKVDEYGAKDYRQQMPLKGD; HTSRPLWVAPDGHIFLEAFSPVYKYAQDFLVAIAEPVCRPTHVHEYKLTAYSLYAAVSVGLQTSDITEYL; RKLSKTGVPDGIIQFIKLCTVSYGKVKLVLKHNRYFVESSHPDVIQHLLQDPVIRECRLRNAEGEATELI; TETFTSKSAISKTAAEGSGGPSTSQGVDAQATSDIPKDLFDFYEQMDKDEEEEEETQTVSFEVKQEMIEE; LQKRCICLEYPLLAEYDFRNDTLNPDINIDLKPTAVLRPYQEKSLRKMFGNGRARSGVIVLPCGAGKSLV; GVTAACTVRKRCLVLGNSAVSVEQWKAQFKMWSTIDDSQICRFTSDAKDKPIGCSVAISTYSMLGHTTKR; SWEAERVMEWLKTQEWGLMILDEVHTIPARMFRRVLTIVQAHCKLGLTATLVREDDKIVDLNFLIGPKLY; EANWMELQNNGYIAKVQCAEVWCPMSPEFYREYVAIKTKKRILLYTMNPNKFRACQFLIKFHERRNDKII; VFADNVFALKEYAIRLNKPYIYGPTSQGERMQILQNFKHNPKINTIFISKVGDTSFDLPEANVLIQISSH; GGSRRQEAQRLGRVLRAKKGMVAEEYNAFFYSLVSQDTQEMAYSTKRQRFLVDQGYSFKVITKLAGMEEE; ELAFSTKEEQQQLLQKVLAATDLDAEEEVVAGEFGSRSGQASRRCGTMSSLSGADDTVYMEYHSSRSKAS; SKHVHPLFKRFRK; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133658
• ORF Size: 2349 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_133658.2
• RefSeq Size: 2673 bp
• RefSeq ORF: 2352 bp
• Locus ID: 13872
• UniProt ID: P49135
• Cytogenetics: 18 B1
• Summary: ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/ERCC3, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB/ERCC3 is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.[UniProtKB/Swiss-Prot Function]