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Gcc1 (NM_028900) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG210602
Gcc1 (tGFP-tagged) - Mouse golgi coiled coil 1 (Gcc1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $913.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gcc1
Synonyms 4932417P04Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG210602 representing NM_028900
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAAGTTTGGGATGAATTTCGGGGGCGGCCCAAGTAAGAAGGATCTCTTGGAGACCATCGAGACAC
AGAAAAAGCAGCTTCTCCAGTACCAGGCAAGACTCAAGGATGTGGTGCGTGCCTATAAAAGCCTGCTGAA
GGAGAAAGAAGCCCTGGAGGCCAGCATCAAGGTACTGTCGGTATCCCACGAGGCGGATGTAGGCCTCTCA
GGTGTGCAGCCTCCAGGTCTCACGTTTCCTGATTCTGTGGATGACCGGTGCTCCACTCACAGTGAGGACA
GCACCGGAACTGCCACTAGCTTGGATACCGCAGCCAGTCTTACAAGTGTCAAGGGTGAATTTGGGGTTGA
AGATGACAGAGCGGCCCGTGGACCACTTCCCCCAAAGTCTGAAGAGGCCAGTGGGTCAGAGAGTGGCGTT
AGTAGTAGCAGTGGGGATGGGCCATCTGCCGGTAGTGAGATGGACAAAAGAGTGCATCAGCTGAAGACTC
AGTTGGCCACTTTGACTAGCTCCTTGGCGACAGTCACTCAGGAAAAGTCCCGTATGGAGGCGTCTTACCT
GGCGGATAAGAAGAAGATGAAGCAGGACTTAGAGGATGCCAATAAAAAGGCAGAGGAAGAGCGGGGTCGC
CTGGAGGGAGACTTGAAGGTGCTGCAAGAGCAGATAGCAGAAACCAAAGCCCGCCTCATTACACAGCAGC
ACGATCGTGCCCAGGAGCAGAGTGACCATGCCTTGATGCTGCGCGAGCTTCAGAAGCTCTTGCAGGAGGA
GAGGACCCAGCGCCAGGACCTGGAACTTCGCTTAGAAGAGACCAGAGAAGCCTTGGCAGGGCGGGCATAT
GCCGCTGATCAGGTCGAAGGGTTTGAACTGCAGACCAAGCAGCTGACCCGAGAGGTAGAGGAGCTGAAAG
GTGAGCTGCAAACCATTCGAGATGAGAAGAACCGACCAGACCCCCGGCTGCAGGAGCTTCAGCAAGAGGC
TGCCCGTCTTAAAAGCCATTTCCAGGCCCAATTGCAGCAAGAAATGAGGAAGACAGCCCTTGCAGAGGAC
CAGCTCCGCCAGCAGTCTCAGGTAGAAGAGCAGAGAGTAGCAGCCCTGGAGAGCCAGATCTCTGAGGTAT
CTGAACTGCTGGGCACCTACGAGAAAGCCAAGCAGAAAGACCAGCTGGCCATCCAGAAGCTGAAGGAGCG
ACTTCTGCAGCTGGACCTGGAGAACAAGACACTGGCCCTCGCAGCCTCTAGCCGGTCCTCCCTTGACATC
CATGGCGACGAATCCAGTCTGGATATCAATGTCTTGAAGGACAAGATGGAGAAGCTCAAGAAGCTACTAC
AGGTTGCAGCTAGGAAAAGCCAGGTGACCCTGGATGTGGAGAAGCTCTGCGACCCTGAGATAATGGCCAA
CTCCGAGGCTGCTGACGGGGAAAAGGCTACCGCGCTCTACTACCAACAGGAGCTGAAGCAGCTGAAGGAA
GAGTTTGAGCGGTATAAGATGCGAGCCCAAGTTGTGCTCAAAAGCAAGAACACCAAAGATGGGAGCCTGG
GGAAGGAGCTGGAGGCAGCACAGGAGCAGCTCGCAGAGCTGAAGGACAAGTATATCTCCCTGCGGCTGTC
CTGTGAGGAGCTGGAAAGCCAGCACCAGCAGGAGGCTGAGGACTGGAAGCAGGAACTGGCCCGGCTGCAG
CAGCTCCATCGGCAGGAACTGGAGCGCAGCCAGCTGGACTTCAGGGACCGCACACTGAAACTGGAGGAGG
AGCTACACAAGCAGCGGGACCGGGCCCTGGCTGTGCTCGCGGAGAAGGACTTGGAGCTGGAGCAACTACG
TTCTGTGGCCTTGTCCTCTGGGTTGCCAGGACGCAGAAGCCCCGTGGGAGGAGTGGGTGGTGGAGGTCTT
GGGGACCCAGCCGACACAGCCTCCTCAGATAGCTTGACCCAGGCCCTGCAGCTTGCTGCAGCCAACGAGC
CCACCTTCTTTCTTTATGCTGAGCAGTTGGCCCGCAAGGAGGTGGAGATCACATCTCTGAGGAAGCAGAA
ACACAGGCTGGAGGTAGAAGCCCATCAGCTGCAGGAAAGGCTGCTGGAGGAGGGCGAGCGGCATCGAGAG
GAGGTTGGAGCCCTCCAGAGCCACATCGAAAAGAATATGAGGGACCAGAGCAGGGAGGGAGCCAACCTGG
AATATCTCAAAAACATCATCTACCGCTTCCTGACCCTGCCCGACAGCCTGGGCCGCCAGCAGACGCTCAC
CGCCATCCTCACGATCTTGCACTTCAGTCCTGAAGAGAAACAAGTGCTAATGCGGCTCCCAAGCGGTGGG
AGCTGGTGGCCTTCCGGCAAGAGA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG210602 representing NM_028900
Red=Cloning site Green=Tags(s)

MEKFGMNFGGGPSKKDLLETIETQKKQLLQYQARLKDVVRAYKSLLKEKEALEASIKVLSVSHEADVGLS
GVQPPGLTFPDSVDDRCSTHSEDSTGTATSLDTAASLTSVKGEFGVEDDRAARGPLPPKSEEASGSESGV
SSSSGDGPSAGSEMDKRVHQLKTQLATLTSSLATVTQEKSRMEASYLADKKKMKQDLEDANKKAEEERGR
LEGDLKVLQEQIAETKARLITQQHDRAQEQSDHALMLRELQKLLQEERTQRQDLELRLEETREALAGRAY
AADQVEGFELQTKQLTREVEELKGELQTIRDEKNRPDPRLQELQQEAARLKSHFQAQLQQEMRKTALAED
QLRQQSQVEEQRVAALESQISEVSELLGTYEKAKQKDQLAIQKLKERLLQLDLENKTLALAASSRSSLDI
HGDESSLDINVLKDKMEKLKKLLQVAARKSQVTLDVEKLCDPEIMANSEAADGEKATALYYQQELKQLKE
EFERYKMRAQVVLKSKNTKDGSLGKELEAAQEQLAELKDKYISLRLSCEELESQHQQEAEDWKQELARLQ
QLHRQELERSQLDFRDRTLKLEEELHKQRDRALAVLAEKDLELEQLRSVALSSGLPGRRSPVGGVGGGGL
GDPADTASSDSLTQALQLAAANEPTFFLYAEQLARKEVEITSLRKQKHRLEVEAHQLQERLLEEGERHRE
EVGALQSHIEKNMRDQSREGANLEYLKNIIYRFLTLPDSLGRQQTLTAILTILHFSPEEKQVLMRLPSGG
SWWPSGKR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028900
ORF Size 2334 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028900.4, NP_083176.3
RefSeq Size 4138 bp
RefSeq ORF 2337 bp
Locus ID 74375
UniProt ID Q9D4H2
Cytogenetics 6 A3.3
Summary Probably involved in maintaining Golgi structure.UniProtKB/Swiss-Prot Function
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"NM_028900" in other vectors (4)
SKU Description Size Price
MC221509 Gcc1 (untagged) - Mouse golgi coiled coil 1 (Gcc1), (10ug) 10 ug
$785.00
MR210602 Gcc1 (Myc-DDK-tagged) - Mouse golgi coiled coil 1 (Gcc1) 10 ug
$589.00 $713.00
MR210602L3 Lenti ORF clone of Gcc1 (Myc-DDK-tagged) - Mouse golgi coiled coil 1 (Gcc1) 10 ug
$1,013.00
MR210602L4 Lenti ORF clone of Gcc1 (mGFP-tagged) - Mouse golgi coiled coil 1 (Gcc1) 10 ug
$1,013.00

Plasmid Map for MG210602

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG210602
Circular map for MG210602

file-alt Citations

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