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Qars (NM_133794) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG210578
Qars (tGFP-tagged) - Mouse glutaminyl-tRNA synthetase (Qars)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $911.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Qars
Synonyms 1110018N24Rik; 1200016L19Rik; C80286; GLNRS
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG210578 representing NM_133794
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTACTCCAGATTCGCTAGCGCTGTTCACCGGCCTTGGCCTTAGCGAGAACAAGGCCCGCGAGACGC
TCAAGAACGAGGCTCTGAGCACTCAGCTGCGCGAGGCGGCGACCCAGGCACACCAGATTCTGGGTTCTAC
CATCGACAAGGCTACTGGGGTCCTCCTATATGACTTGGTCTCCCGACTCAGGGATACTCGGCGTCGTTCT
TTCCTTGTGAGCTATATAGCCAATAAGAAGATTCACACGGGACTCCAGCTGAGCGCTGCTCTTGAATATG
TTCGGAGTCATCCCCAGGATCCCATTGATACCAAGGACTTCGAGCAGGAGTGTGGCGTCGGTGTGGTGGT
GACACCGGAGCAGATTGAGGAAGCTGTGGAGTCCACCATAAATAAGCATCAGTTGCAGCTCCTAGCGGAA
CGGTACCGTTTCAACATGGGGCTGCTAATGGGAGAGGCTCGGGCTGCGCTCAGATGGGCAGACGGCAAAA
TGATCAAGAACGAGGTGGATATGCAGGTGCTTCACCTTTTGGGGCCCAAGATGGAAGCTGATCTGGTGAA
GAAGCCCAAGGTGGCAAAGGCACGGCTGGAAGAAACAGACCGGAAGACAGCAAAAGATGTGGTGGAGAAA
GGTGAAGTGGCTGGCCAGACCCTGTCTCTGATGGAGCAGCTCCGGGGGGAGGCACTTAAGTTTCATAAGC
CAGGTGAGAACTACAAGACGCCAGGCTATGTGATCACTCCATATACCATGGATCTGCTGAAGCAGCACCT
GGAGATCACTGGGGGACAGGTACGTACGCGGTTCCCTCCAGAGCCCAATGGAATCCTGCATATTGGACAC
GCCAAAGCCATCAATTTCAACTTTGGTTATGCCAAGGCCAACAATGGTATCTGTTTTCTGCGCTTTGATG
ACACCAACCCTGAGAAAGAAGAAGCAAAATTCTTCACTGCTATTTATGACATGGTGACCTGGCTGGGTTA
TACACCTTACAAAGTGACATATGCTTCTGACTATTTTGACCAGCTGTATGCCTGGGCCGTGGAACTCATC
CATGGGGGTCTTGCTTATGTGTGTCACCAGAGAGTGGAAGAGCTCAAAGGCCATAACCCTTTACCTTCTC
CATGGAGGGACAGGCCTAAGGAAGAATCATTGCTGCTCTTTGAGGCAATGCGCAAGGGCAAATTTGCAGA
AGGAGAGGCCACACTTCGAATGAAGTTGGTGATGGAAGATGGCAAGATGGACCCCGTGGCCTATCGAGTC
AAGTACACGCCACACCATCGCACAGGGGACAAATGGTGCATCTACCCCACCTATGACTACACACATTGTC
TGTGTGACTCCATTGAGCACATTACCCACTCGTTGTGTACCAAGGAGTTCCAGGCTCGACGGTCTTCCTA
CTTTTGGTTATGTAACGCTCTGAAAGTCTATTGTCCTGTTCAGTGGGAATATGGTCGCCTCAATTTGCAC
TATGCTGTTGTCTCAAAGCGCAAGATTCTCCAGCTTGTAGCAGCTGGTGCTGTTCGGGACTGGGACGATC
CACGGCTCTTCACACTCACTGCCCTACGACGACGGGGTTTTCCACCAGAGGCTATCAACAACTTCTGTGC
TCGGGTTGGGGTCACAGTGGCACAGACCACAATGGAACCTCATCTTCTGGAAGCCTGTGTGCGTGATGTG
CTGAATGATGCAGCCCCACGTGCCATGGCTGTTCTAGAGCCACTACAAGTTGTCATCACCAACTTTCCTG
CTCCCAAGCCCTTGGACATCCGAGTGCCAAATTTCCCAGCTGATGAGACCAAGGGTTTCCACCAGGTTCC
TTTTGCTTCCACTGTCTTCATTGAGAGAAGCGACTTTAAGGAGGAGTCAGAACCAGGCTATAAGCGCCTA
GCTTCGGGCCAGCCTGTGGGCCTGAGACACACTGGTTATGTCATTGAACTGCAGAATATTGTCAGGGGCT
CCAGTGGCTGTGTGGAACGCTTGGAGGTGACCTGTAGACGAGCTGATGCTGGAGAGAAGCCCAAGGCCTT
TATTCACTGGGTATCACAGCCTTTGGTGTGTGAGATTCGCCTCTATGAGTGCCTATTCCAGCACAAGAAC
CCCGAAGACCCTGTTGAAGTGCCTGGTGGATTCCTAAGTGACCTGAACCCGGCGTCACTACAAGTGGTAG
AAGGAGCATTAGTGGACTGCTCTGTGGCTTTGGCAAAGCCCTTTGACAAGTTCCAGTTTGAGCGCCTTGG
GTACTTCTCTGTGGATCCAGACAGCCATCAAGGACAGATTGTCTTCAACCGAACTGTCACACTAAAAGAA
GACCCAGGCAAAATA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG210578 representing NM_133794
Red=Cloning site Green=Tags(s)

MATPDSLALFTGLGLSENKARETLKNEALSTQLREAATQAHQILGSTIDKATGVLLYDLVSRLRDTRRRS
FLVSYIANKKIHTGLQLSAALEYVRSHPQDPIDTKDFEQECGVGVVVTPEQIEEAVESTINKHQLQLLAE
RYRFNMGLLMGEARAALRWADGKMIKNEVDMQVLHLLGPKMEADLVKKPKVAKARLEETDRKTAKDVVEK
GEVAGQTLSLMEQLRGEALKFHKPGENYKTPGYVITPYTMDLLKQHLEITGGQVRTRFPPEPNGILHIGH
AKAINFNFGYAKANNGICFLRFDDTNPEKEEAKFFTAIYDMVTWLGYTPYKVTYASDYFDQLYAWAVELI
HGGLAYVCHQRVEELKGHNPLPSPWRDRPKEESLLLFEAMRKGKFAEGEATLRMKLVMEDGKMDPVAYRV
KYTPHHRTGDKWCIYPTYDYTHCLCDSIEHITHSLCTKEFQARRSSYFWLCNALKVYCPVQWEYGRLNLH
YAVVSKRKILQLVAAGAVRDWDDPRLFTLTALRRRGFPPEAINNFCARVGVTVAQTTMEPHLLEACVRDV
LNDAAPRAMAVLEPLQVVITNFPAPKPLDIRVPNFPADETKGFHQVPFASTVFIERSDFKEESEPGYKRL
ASGQPVGLRHTGYVIELQNIVRGSSGCVERLEVTCRRADAGEKPKAFIHWVSQPLVCEIRLYECLFQHKN
PEDPVEVPGGFLSDLNPASLQVVEGALVDCSVALAKPFDKFQFERLGYFSVDPDSHQGQIVFNRTVTLKE
DPGKI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_133794
ORF Size 2325 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_133794.2
RefSeq Size 2404 bp
RefSeq ORF 2328 bp
Locus ID 97541
UniProt ID Q8BML9
Cytogenetics 9 F2
Summary Glutamine--tRNA ligase. Plays a critical role in brain development.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_133794" in other vectors (3)
SKU Description Size Price
MR210578 Qars (Myc-DDK-tagged) - Mouse glutaminyl-tRNA synthetase (Qars), transcript variant 1 10 ug
$589.00 $711.00
MR210578L3 Lenti ORF clone of Qars (Myc-DDK-tagged) - Mouse glutaminyl-tRNA synthetase (Qars), transcript variant 1 10 ug
$1,011.00
MR210578L4 Lenti ORF clone of Qars (mGFP-tagged) - Mouse glutaminyl-tRNA synthetase (Qars), transcript variant 1 10 ug
$1,011.00

Plasmid Map for MG210578

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG210578
Circular map for MG210578

file-alt Citations

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