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Afap1 (NM_027373) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG210338
Afap1 (tGFP-tagged) - Mouse actin filament associated protein 1 (Afap1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $869.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Afap1
Synonyms 2600003E23Rik; 9630044L16Rik; Afap; AI848729; mKIAA3018
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG210338 representing NM_027373
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGAGTTAATAGTTGAACTCCGCCTCTTTCTGGAGCTCCTGGACCACGAGTACCTCACATCAACTG
TCAGAGAGAAAAAGGCTGTGCTCACCAACATCCTGCTGCGCCTGCAGTCATCCAAGGGCTTTGAAGTGAA
GGACCATGCTCAGAAGGCAGAGGCCAACAACCTGCCGGCACCACCCCAGATGCCTCTGCCGGAGATCCCT
CAGCCATGGCTGCCTCCTGACAGCGGGCCTCCGCCTTTACCAACGTCCTCTCTCCCAGAGGGTTACTATG
AGGAAGCCGTGCCGCTGAGCCCTGGGAAAGCTCCAGAGTACATCACCTCAAATTATGACTCTGATGCCAT
GAGCAGTTCCTATGAGTCATATGATGAAGAGGAAGAGGACGGGAAGGGGAAGAAAACTCGGCATCAGTGG
CCGTCAGAGGAGGCCTCTATGGACCTGGTGAAAGACGCCAAGATCTGTGCCTTCCTGCTGCGCAAGAAAC
GCTTTGGCCAGTGGACCAAGCTGCTCTGCGTCATCAAAGACACCAAGCTGCTGTGCTATAAAAGTTCCAA
GGACCAGCAGCCTCAGATGGAGCTGCCGCTGCAGGGCTGCAGTATCACATACATCCCGAGAGACAGCAAG
AAGAAGAAACACGAGCTGAAAATCACCCAGCAAGGCACAGACCCTCTGGTTCTAGCTGTTCAGAGCAAGG
AACAGGCTGAGCAGTGGTTGAAGGTAATCAAAGAAGCCTACAGTGGCTGTAGCGGGCCCGTGGATCCGGA
ATGTTCTCCACCACCCAGCACCAGCGCCCCCGTGAACAAGGCAGAACTGGAGAAGAAATTGTCTTCAGAG
AGGCCCAGCTCTGATGGAGAAGGTGGTGTGGAAAACGGAGTCACCACGTGTAATGGAAAAGAACAAGCAA
AGAGGAAGAAACCTTCCAAGTCAGAGGCCAAGGGGACTGTGTCCAAGGTCACTGGGAAGAAAATTACCAA
GATCATCGGTCTGGGAAAGAAGAAGCCATCCACAGATGAGCAGACATCATCAGCAGAGGAGGATGTGCCC
ACTTGTGGCTACCTGAATGTGCTCTCTAACAGCCGCTGGCGGGAGCGCTGGTGCAGAGTGAAGGACAGCA
AACTCATTCTCCACAAGGACAGGGCTGACCTGAAGACCCACCTCGTCTCCATCCCCCTCCGTGGCTGCGA
GGTGATCCCGGGCCTGGATTCTAAGCACCCGTTGACCTTCCGGCTGCTTCGAAACGGACAGGAGGTGGCT
GTTCTAGAGGCATCATCGTCTGAAGACATGGGCAGGTGGATTGGCATCTTGCTGGCTGAGACGGGGTCCT
CCACGGACCCAGGAGCGCTGCACTACGACTACATCGATGTGGAGATGTCAGCAAATGTCATCCAGACGGC
GAAACAGACCTTCTGCTTTATGAACAGGCGGGCAGTGTCCACTAGCCCATACCTGGGGAGCCTCTCCAAC
GGCTACGCCCATCCCAGTGGGACAGCACTACACTATGATGACGTCCCCTGTGTCAACGGCTCGTTGAAGA
ACAAAAAACCGCCAGCGTCATCTAATGGGGTTCCTGTAAAGGGGAAGGCTCCCAGCAGTCAGCAAAAAAA
GGTGGAAACTGCAGGTGGTGTGAAACGGACAGCATCAAATGCTGAGCAGTACAAATATGGGAAGAACCGG
GTAGAAGCAGATGCCAAGCGGTTACAGTCCAAAGAGGAAGAGCTGCTGAAGAGGAAAGAGGCCCTACGGA
ACAGGCTGGCACAGCTGCGGAAAGAGAGGAAGGACCTGAGGGCCGCCATCGAAGTGAATGCAGGCAGGAA
GACCCAGGCAGCTCTGGAGGACAAGTTGAAGAGGCTGGAGGAAGAGTGCAAGCAGAGGGAGGCGGAGCGT
GTCAGCCTGGAGCTGGAGCTGACCGAGGTCAAGGAGAGCCTGAAGAAGGCCTTGGCGGGTGGCGTCACCC
TAGGACTGGCCATCGAGCCCAGGTCAGGGACATCCAGTCCACAGTCTCCTGTGTTTCGGCATCGGACTCT
GGAAAATTCTCCCATCTCCAGCTGTGATACAAGCGATGCTGAGGGGCCTTTGCCTGTGAACAGTGCAGCC
GTCCTGAAGAAGAGCCAGCCGTCCTCAGGCAGCTCCCCTTGCCGTGGCCACGTGCTGCAGAAGGCCAAGG
AGTGGGAGCTGAAGAATGGGACA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG210338 representing NM_027373
Red=Cloning site Green=Tags(s)

MEELIVELRLFLELLDHEYLTSTVREKKAVLTNILLRLQSSKGFEVKDHAQKAEANNLPAPPQMPLPEIP
QPWLPPDSGPPPLPTSSLPEGYYEEAVPLSPGKAPEYITSNYDSDAMSSSYESYDEEEEDGKGKKTRHQW
PSEEASMDLVKDAKICAFLLRKKRFGQWTKLLCVIKDTKLLCYKSSKDQQPQMELPLQGCSITYIPRDSK
KKKHELKITQQGTDPLVLAVQSKEQAEQWLKVIKEAYSGCSGPVDPECSPPPSTSAPVNKAELEKKLSSE
RPSSDGEGGVENGVTTCNGKEQAKRKKPSKSEAKGTVSKVTGKKITKIIGLGKKKPSTDEQTSSAEEDVP
TCGYLNVLSNSRWRERWCRVKDSKLILHKDRADLKTHLVSIPLRGCEVIPGLDSKHPLTFRLLRNGQEVA
VLEASSSEDMGRWIGILLAETGSSTDPGALHYDYIDVEMSANVIQTAKQTFCFMNRRAVSTSPYLGSLSN
GYAHPSGTALHYDDVPCVNGSLKNKKPPASSNGVPVKGKAPSSQQKKVETAGGVKRTASNAEQYKYGKNR
VEADAKRLQSKEEELLKRKEALRNRLAQLRKERKDLRAAIEVNAGRKTQAALEDKLKRLEEECKQREAER
VSLELELTEVKESLKKALAGGVTLGLAIEPRSGTSSPQSPVFRHRTLENSPISSCDTSDAEGPLPVNSAA
VLKKSQPSSGSSPCRGHVLQKAKEWELKNGT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027373
ORF Size 2193 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027373.2, NP_081649.1
RefSeq Size 6617 bp
RefSeq ORF 2196 bp
Locus ID 70292
UniProt ID Q80YS6
Cytogenetics 5 B3
Summary Can cross-link actin filaments into both network and bundle structures. May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton (By similarity).UniProtKB/Swiss-Prot Function
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"NM_027373" in other vectors (4)
SKU Description Size Price
MC204907 Afap1 (untagged) - Mouse actin filament associated protein 1 (Afap1), (10ug) 10 ug
$670.00
MR210338 Afap1 (Myc-DDK-tagged) - Mouse actin filament associated protein 1 (Afap1) 10 ug
$589.00 $669.00
MR210338L3 Lenti ORF clone of Afap1 (Myc-DDK-tagged) - Mouse actin filament associated protein 1 (Afap1) 10 ug
$969.00
MR210338L4 Lenti ORF clone of Afap1 (mGFP-tagged) - Mouse actin filament associated protein 1 (Afap1) 10 ug
$969.00

Plasmid Map for MG210338

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG210338
Circular map for MG210338

file-alt Citations

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