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Afap1 (NM_027373) Mouse Untagged Clone

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SKU
MC204907
Afap1 (untagged) - Mouse actin filament associated protein 1 (Afap1), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $670.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Afap1
Synonyms 2600003E23Rik; 9630044L16Rik; Afap; AI848729; mKIAA3018
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC050814
GCTGTTCAGGGGGCGGGGCTATGGAGGCAGGCGGGGCCTGTCGCGCCTGCGCAGAATCCTCCGGCCACAG CTCGCACCGGCGGTGTCTGGGCTCCAGGTCCCGACTGAGAGATCGCTGAGCTGCGCAGGGCCCCGCCCCG GGCTCCATTGTTGAGGCAGCGGGCGCTCCGCGGGCTGCGCTCCTCTCGGCCGTCCAGTGCTCACGAGCCA CTGAATCCTGAGCAATGGAAGAGTTAATAGTTGAACTCCGCCTCTTTCTGGAGCTCCTGGACCACGAGTA CCTCACATCAACTGTCAGAGAGAAAAAGGCTGTGCTCACCAACATCCTGCTGCGCCTGCAGTCATCCAAG GGCTTTGAAGTGAAGGACCATGCTCAGAAGGCAGAGGCCAACAACCTGCCGGCACCACCCCAGATGCCTC TGCCGGAGATCCCTCAGCCATGGCTGCCTCCTGACAGCGGGCCTCCGCCTTTACCAACGTCCTCTCTCCC AGAGGGTTACTATGAGGAAGCCGTGCCGCTGAGCCCTGGGAAAGCTCCAGAGTACATCACCTCAAATTAT GACTCTGATGCCATGAGCAGTTCCTATGAGTCATATGATGAAGAGGAAGAGGACGGGAAGGGGAAGAAAA CTCGGCATCAGTGGCCGTCAGAGGAGGCCTCTATGGACCTGGTGAAAGACGCCAAGATCTGTGCCTTCCT GCTGCGCAAGAAACGCTTTGGCCAGTGGACCAAGCTGCTCTGCGTCATCAAAGACACCAAGCTGCTGTGC TATAAAAGTTCCAAGGACCAGCAGCCTCAGATGGAGCTGCCGCTGCAGGGCTGCAGTATCACATACATCC CGAGAGACAGCAAGAAGAAGAAACACGAGCTGAAAATCACCCAGCAAGGCACAGACCCTCTGGTTCTAGC TGTTCAGAGCAAGGAACAGGCTGAGCAGTGGTTGAAGGTAATCAAAGAAGCCTACAGTGGCTGTAGCGGG CCCGTGGATCCGGAATGTTCTCCACCACCCAGCACCAGCGCCCCCGTGAACAAGGCAGAACTGGAGAAGA AATTGTCTTCAGAGAGGCCCAGCTCTGATGGAGAAGGTGGTGTGGAAAACGGAGTCACCACGTGTAATGG AAAAGAACAAGCAAAGAGGAAGAAACCTTCCAAGTCAGAGGCCAAGGGGACTGTGTCCAAGGTCACTGGG AAGAAAATTACCAAGATCATCGGTCTGGGAAAGAAGAAGCCATCCACAGATGAGCAGACATCATCAGCAG AGGAGGATGTGCCCACTTGTGGCTACCTGAATGTGCTCTCTAACAGCCGCTGGCGGGAGCGCTGGTGCAG AGTGAAGGACAGCAAACTCATTCTCCACAAGGACAGGGCTGACCTGAAGACCCACCTCGTCTCCATCCCC CTCCGTGGCTGCGAGGTGATCCCGGGCCTGGATTCTAAGCACCCGTTGACCTTCCGGCTGCTTCGAAACG GACAGGAGGTGGCTGTTCTAGAGGCATCATCGTCTGAAGACATGGGCAGGTGGATTGGCATCTTGCTGGC TGAGACGGGGTCCTCCACGGACCCAGGAGCGCTGCACTACGACTACATCGATGTGGAGATGTCAGCAAAT GTCATCCAGACGGCGAAACAGACCTTCTGCTTTATGAACAGGCGGGCAGTGTCCACTAGCCCATACCTGG GGAGCCTCTCCAACGGCTACGCCCATCCCAGTGGGACAGCACTACACTATGATGACGTCCCCTGTGTCAA CGGCTCGTTGAAGAACAAAAAACCGCCAGCGTCATCTAATGGGGTTCCTGTAAAGGGGAAGGCTCCCAGC AGTCAGCAAAAAAAGGTGGAAACTGCAGGTGGTGTGAAACGGACAGCATCAAATGCTGAGCAGTACAAAT ATGGGAAGAACCGGGTAGAAGCAGATGCCAAGCGGTTACAGTCCAAAGAGGAAGAGCTGCTGAAGAGGAA AGAGGCCCTACGGAACAGGCTGGCACAGCTGCGGAAAGAGAGGAAGGACCTGAGGGCCGCCATCGAAGTG AATGCAGGCAGGAAGACCCAGGCAGCTCTGGAGGACAAGTTGAAGAGGCTGGAGGAAGAGTGCAAGCAGA GGGAGGCGGAGCGTGTCAGCCTGGAGCTGGAGCTGACCGAGGTCAAGGAGAGCCTGAAGAAGGCCTTGGC GGGTGGCGTCACCCTAGGACTGGCCATCGAGCCCAGGTCAGGGACATCCAGTCCACAGTCTCCTGTGTTT CGGCATCGGACTCTGGAAAATTCTCCCATCTCCAGCTGTGATACAAGCGATGCTGAGGGGCCTTTGCCTG TGAACAGTGCAGCCGTCCTGAAGAAGAGCCAGCCGTCCTCAGGCAGCTCCCCTTGCCGTGGCCACGTGCT GCAGAAGGCCAAGGAGTGGGAGCTGAAGAATGGGACATAGAGGGAGCCCAGCCACGCCCAGAGACTGTGT ACTGCCACCAGCCTCGCGTGTGACACGTGTGCCTCCCAGAGTGGCCTGAACATGACCACAGAAGTTTGGA CGAGTCTGACGCTGACCCTTGGCTGGCTTGCGTGCACGCTTTCTACTGTATGACGGTGCCCTTTAGGAAA GTTTACTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites SfiI-SfiI |
ACCN NM_027373
Insert Size 2196 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC050814, AAH50814
RefSeq Size 2629 bp
RefSeq ORF 2196 bp
Locus ID 70292
UniProt ID Q80YS6
Cytogenetics 5 B3
Summary Can cross-link actin filaments into both network and bundle structures. May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton (By similarity).UniProtKB/Swiss-Prot Function
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Documents
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"NM_027373" in other vectors (4)
SKU Description Size Price
MG210338 Afap1 (tGFP-tagged) - Mouse actin filament associated protein 1 (Afap1) 10 ug
$869.00
MR210338 Afap1 (Myc-DDK-tagged) - Mouse actin filament associated protein 1 (Afap1) 10 ug
$589.00 $669.00
MR210338L3 Lenti ORF clone of Afap1 (Myc-DDK-tagged) - Mouse actin filament associated protein 1 (Afap1) 10 ug
$969.00
MR210338L4 Lenti ORF clone of Afap1 (mGFP-tagged) - Mouse actin filament associated protein 1 (Afap1) 10 ug
$969.00

file-alt Citations

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