OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Gne (NM_015828) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MG210281
Gne (tGFP-tagged) - Mouse glucosamine (Gne)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $862.00
3 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (4)

TA150041 Mouse monoclonal turboGFP antibody, clone OTI2H8 100 ul
$447.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100010 pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
10 ug
$780.00

Other products for "Gne or NM_015828"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gne
Synonyms 2310066H07Rik; DMRV; GLCNE; IBM2; NM; Uae1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG210281 representing NM_015828
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAAGAACGGGAACAACCGAAAGCTCCGGGTTTGCGTTGCCACCTGCAACCGAGCTGACTACTCCA
AACTGGCCCCGATCATGTTCGGCATCAAGACAGAGCCCGCGTTCTTTGAGTTGGACGTGGTGGTGCTCGG
CTCCCACCTGATTGACGACTATGGAAACACATACCGCATGATTGAGCAAGATGACTTTGACATTAACACC
AGGCTCCACACGATTGTTAGAGGGGAAGATGAAGCGGCCATGGTAGAGTCGGTAGGCCTAGCGCTCGTGA
AGCTACCGGACGTCCTCAATCGCCTGAAGCCCGACATCATGATTGTTCACGGAGACCGATTTGACGCCCT
TGCTCTGGCTACGTCTGCTGCCTTGATGAACATCCGCATCCTTCACATTGAAGGAGGCGAGGTCAGCGGG
ACCATTGATGACTCTATCAGACACGCCATAACAAAACTGGCTCACTACCATGTGTGCTGCACTAGAAGTG
CAGAGCAGCACCTGATCTCTATGTGCGAGGACCACGACCGCATCCTGTTGGCAGGCTGCCCTTCCTATGA
CAAACTGCTCTCCGCCAAGAACAAAGACTATATGAGCATCATTCGGATGTGGCTAGGCGATGATGTAAAA
TGTAAGGATTACATCGTTGCCCTGCAGCATCCCGTGACCACTGACATTAAGCATTCCATAAAGATGTTTG
AGCTAACACTGGATGCCCTGATCTCGTTTAACAAGAGGACCCTAGTTCTGTTTCCAAATATCGATGCAGG
CAGCAAGGAGATGGTTCGAGTGATGCGGAAGAAGGGCATCGAGCATCACCCCAATTTCCGTGCAGTCAAG
CACGTCCCGTTTGACCAGTTCATACAGCTGGTCGCCCACGCTGGCTGCATGATTGGGAATAGCAGCTGCG
GCGTGCGAGAGGTTGGCGCTTTCGGAACACCCGTGATCAACCTGGGCACAAGGCAGATAGGAAGAGAAAC
CGGGGAGAATGTTCTTCATGTCAGGGATGCTGACACCCAAGATAAAATATTGCAAGCACTACACCTCCAG
TTCGGCAAACAGTACCCTTGCTCAAAGATATATGGGGATGGGAATGCTGTTCCAAGGATTTTAAAGTTTC
TCAAATCCATTGACCTTCAAGAGCCACTACAGAAGAAATTCTGCTTCCCCCCTGTAAAGGAGAACATCTC
TCAAGACATTGACCACATCCTGGAAACTCTGAGTGCCTTGGCTGTTGATCTTGGCGGGACAAACCTGAGG
GTGGCAATAGTTAGCATGAAGGGTGAAATCGTTAAGAAGTACACTCAGTTCAACCCTAAAACCTATGAAG
AAAGGATTAGTTTAATCCTGCAGATGTGTGTGGAAGCTGCCGCGGAAGCTGTGAAACTCAATTGCAGAAT
TCTGGGAGTAGGCATCTCCACAGGTGGCCGCGTGAATCCCCAGGAAGGAGTTGTGCTGCATTCAACCAAG
CTGATCCAGGAATGGAACTCCGTGGACCTCAGGACACCCCTCTCCGACACCCTGCATCTCCCCGTGTGGG
TGGACAATGACGGCAACTGTGCCGCCATGGCAGAGAGGAAGTTCGGCCAAGGAAAAGGACAGGAGAACTT
CGTGACGCTCATCACGGGGACAGGGATCGGTGGGGGGATCATCCACCAGCACGAACTGATCCACGGCAGC
TCCTTCTGCGCGGCGGAGCTCGGCCATCTCGTGGTGTCCCTGGACGGTCCTGACTGCTCCTGTGGAAGCC
ATGGGTGCATCGAAGCGTACGCCTCTGGAATGGCCTTGCAGAGGGAAGCAAAGAAACTCCATGATGAGGA
CCTGCTCTTGGTGGAAGGGATGTCAGTACCAAAAGACGAAGCTGTGGGTGCCCTCCATCTCATCCAGGCT
GCCAAGCTGGGCAACGTGAAGGCCCAGAGCATCTTACGAACAGCTGGAACTGCTTTGGGACTTGGGGTTG
TGAACATCCTCCACACTATGAATCCTTCCCTGGTGATCCTGTCTGGAGTCCTGGCCAGTCACTACATCCA
CATCGTGAAGGACGTCATCCGCCAGCAAGCCTTGTCCTCCGTGCAGGATGTGGACGTGGTGGTCTCAGAC
TTGGTGGACCCGGCCCTGCTTGGCGCAGCCAGCATGGTTCTGGACTACACAACGCGCAGGATCCAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG210281 representing NM_015828
Red=Cloning site Green=Tags(s)

MEKNGNNRKLRVCVATCNRADYSKLAPIMFGIKTEPAFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINT
RLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSG
TIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVK
CKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVK
HVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQ
FGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLR
VAIVSMKGEIVKKYTQFNPKTYEERISLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPQEGVVLHSTK
LIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAAMAERKFGQGKGQENFVTLITGTGIGGGIIHQHELIHGS
SFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVGALHLIQA
AKLGNVKAQSILRTAGTALGLGVVNILHTMNPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSD
LVDPALLGAASMVLDYTTRRIH

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015828
ORF Size 2166 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015828.2, NP_056643.2
RefSeq Size 2654 bp
RefSeq ORF 2262 bp
Locus ID 50798
UniProt ID Q91WG8
Cytogenetics 4 B1
Summary Regulates and initiates biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. Required for normal sialylation in hematopoietic cells (By similarity). Sialylation is implicated in cell adhesion, signal transduction, tumorigenicity and metastatic behavior of malignant cells. Plays an essential role in early development.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_015828" in other vectors (4)
SKU Description Size Price
MC205887 Gne (untagged) - Mouse glucosamine (Gne), transcript variant 2, (10ug) 10 ug
$662.00
MR210281 Gne (Myc-DDK-tagged) - Mouse glucosamine (Gne), transcript variant 2 10 ug
$589.00 $662.00
MR210281L3 Lenti ORF clone of Gne (Myc-DDK-tagged) - Mouse glucosamine (Gne), transcript variant 2 10 ug
$962.00
MR210281L4 Lenti ORF clone of Gne (mGFP-tagged) - Mouse glucosamine (Gne), transcript variant 2 10 ug
$962.00

Plasmid Map for MG210281

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG210281
Circular map for MG210281

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products