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Elmo3 (NM_172760) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG210272
Elmo3 (tGFP-tagged) - Mouse engulfment and cell motility 3, ced-12 homolog (C
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $859.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Elmo3
Synonyms 9930107J06; BC058752; CED-12; Ced12
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG210272 representing NM_172760
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCCCCCGCGGAATGTGGTGAAGATTGCTGTTCAGATGTCTGACGCCATCCCACAGCTTATCCAGC
TAGACCAGGCAAAGCCCCTGGCCACTGTACTGAAGGAGGTGTGTGACGCGTGGAGCCTGACTCATCCGGA
GCACTATGCCCTGCAGTTTGCTGATGGGCACAGGAAATACATCACAGAGAATAACCGCTTGGAGATCAAG
AATGGAAGCATCCTGTGTCTCAGCACTGCCCCAGATCTGAAGGCCCAGCAGCTACTGGGTAGACTGCAGA
ATACAAGTCGTGAAGGGTGCTGTGAAGTCCTGAGAAACCTGGTCCTGCTGGCCTCAGACATGACCTTTGC
CCAGGAGGTCATCAGTCGTGATGGGCTTCAGAAACTAAGCACCATCATTGAAAATGGGGATGACCTAGGG
GAGATGCTGGCCCTTGGTCTAAGGGCTTTCTTGGAGCTCATGGAACACGGTGTAGTGTCCTGGGAGACAC
TCAGCATCTCCTTTGTCAGGAAGGTGATATCTTATGTGAACATGAACCTGATGGATGCCTCTGTCCAGCC
CCTGGCTCTGAGACTGCTGGAGAGTGTGACCTTGAGCAGTCCTGCCCTGGGCCAGCTGGTGAAGAGTGAG
GTGCCACTGGATAGGCTGCTGGTGCACCTGCAAGTAATGAACCACCAGCTGCAAACCAAGGCCATGGCTC
TGTTGACGGCCCTGCTGCAGGGAGCCAGCCCCACTGAACGCAAGGAAATGCTTGACCACCTATGGAAGAA
GAATCTTCGCCAGTTCATCTACAAGAACATCATCCACAGTGCAACACCCATGGGTGACGAGATGGCTCAT
CACTTGTATGTGCTGCAGGCTCTTACCCTAGGGCTGCTGGAGCCACGGATGCGGACACCACTTGATCCCT
ACAGCCAGGAACAGCGGGACCAGCTGCAGGCTCTGCGCCAGGCAGCCTTTGAACCAGAGGGGGAGTCCTT
GGGCACAGGGCTCAGTGCAGACCGCCGCCGGTCTCTCTGTGTCCGAGAGTTCCGCAAGTTAGGCTTCTCT
AACAGCAACCCAGCCCAGGACCTGGAGCGTGTGCCCCCCGGCCTGCTGGCCCTGGACAATATGCTCTACT
TCTCCAGACATGCACCAAGTGCATACAGTCGGTTTGTGTTGGAGAACAGTAGCCGAGAGGACAAGCATGA
ATGCCCCTTTGCCCGGAGCAGCATCCAGCTGACAGCGTTGCTGTGTGAGCTGCTCCGTGTTGGGGAGCCT
TGCTCCGAGACAGCCCAGGACTTCTCGCCCATGTTCTTCAGCCAAGACCACAGTTTCCATGAGCTCTTCT
GTGTGGCTATCCAGCTACTGAATAAGACCTGGAAGGAGATGCGGGCAACACAGGAAGATTTTGACAAGGT
GATGCAAGTGGTTCGGGAGCAGCTGGCCCGTACCCTGGCTCTGAAGCCCACCTCCCTGGAGCTCTTTCGA
ACCAAAGTGAATGCCCTCACCTATGGGGAAGTGTTGAGGCTGCGGCAGACAGAGCGGCTGCACCAGGAGG
GCACGCTGGCCCCTCCTATACTGGAATTGCGAGAGAAACTGAAGCCAGAGCTCATGGGCTTAATCCGCCA
GCAGCGTTTGCTCCGACTCTGTGAAGGAATGCTCTTCCGCAAGATCAGCAGCCGGAGACGACAGGACAAG
CTATGGTTTTGCTGTTTATCCCCCAACCACAAAGTACTGCAGTATGGGGATGTGGAGGAGGGTGCCAAGC
CCCCTACCCTAGAAAGCCTACCTGAACAGCTCCCTGTGGCAGACATCAGGGCACTCCTAATGGGCAAGGA
CTGCCCCCATGTCCGGGAGAAGGGCTCTGGGAAGCAGAACAAGGACCTGTATGAGCTGGCTTTCTCCATC
AGCTATGACCACGGGGAGGAAGAAGCATACCTCAACTTCATTGCCCCCTCCAAACGGGATTTCTACCTGT
GGACAGACGGGCTAAGTGCCCTGCTGGGCAGTACCATGGGCAGTGAGCTGACTCGGCTAGACCTGGAGCA
GCTGCTCACTATGGAGACCAAGTTGCGGTTGTTGGAACTGGAGAACGTGCCCATTCCTGAGCAGCCTCCC
CCAGTACCCCCACCCCCTACCAACTTTAACTTCTGCTATGACTACAGCATCACTGAGCCG

AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG210272 representing NM_172760
Red=Cloning site Green=Tags(s)

MAPPRNVVKIAVQMSDAIPQLIQLDQAKPLATVLKEVCDAWSLTHPEHYALQFADGHRKYITENNRLEIK
NGSILCLSTAPDLKAQQLLGRLQNTSREGCCEVLRNLVLLASDMTFAQEVISRDGLQKLSTIIENGDDLG
EMLALGLRAFLELMEHGVVSWETLSISFVRKVISYVNMNLMDASVQPLALRLLESVTLSSPALGQLVKSE
VPLDRLLVHLQVMNHQLQTKAMALLTALLQGASPTERKEMLDHLWKKNLRQFIYKNIIHSATPMGDEMAH
HLYVLQALTLGLLEPRMRTPLDPYSQEQRDQLQALRQAAFEPEGESLGTGLSADRRRSLCVREFRKLGFS
NSNPAQDLERVPPGLLALDNMLYFSRHAPSAYSRFVLENSSREDKHECPFARSSIQLTALLCELLRVGEP
CSETAQDFSPMFFSQDHSFHELFCVAIQLLNKTWKEMRATQEDFDKVMQVVREQLARTLALKPTSLELFR
TKVNALTYGEVLRLRQTERLHQEGTLAPPILELREKLKPELMGLIRQQRLLRLCEGMLFRKISSRRRQDK
LWFCCLSPNHKVLQYGDVEEGAKPPTLESLPEQLPVADIRALLMGKDCPHVREKGSGKQNKDLYELAFSI
SYDHGEEEAYLNFIAPSKRDFYLWTDGLSALLGSTMGSELTRLDLEQLLTMETKLRLLELENVPIPEQPP
PVPPPPTNFNFCYDYSITEP

SGPTRTRRLE - GFP Tag - V
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_172760
ORF Size 2160 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172760.3, NP_766348.3
RefSeq Size 3060 bp
RefSeq ORF 2163 bp
Locus ID 234683
UniProt ID Q8BYZ7
Cytogenetics 8 D3
Summary Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1 (By similarity).UniProtKB/Swiss-Prot Function
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"NM_172760" in other vectors (4)
SKU Description Size Price
MC203311 Elmo3 (untagged) - Mouse engulfment and cell motility 3, ced-12 homolog (C. elegans) (Elmo3), (10ug) 10 ug
$660.00
MR210272 Elmo3 (Myc-DDK-tagged) - Mouse engulfment and cell motility 3, ced-12 homolog (C. elegans) (Elmo3) 10 ug
$589.00 $659.00
MR210272L3 Lenti ORF clone of Elmo3 (Myc-DDK-tagged) - Mouse engulfment and cell motility 3, ced-12 homolog (C. elegans) (Elmo3) 10 ug
$959.00
MR210272L4 Lenti ORF clone of Elmo3 (mGFP-tagged) - Mouse engulfment and cell motility 3, ced-12 homolog (C. elegans) (Elmo3) 10 ug
$959.00

Plasmid Map for MG210272

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG210272
Circular map for MG210272

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