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Pgghg (NM_145387) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG210060
Athl1 (tGFP-tagged) - Mouse ATH1, acid trehalase-like 1 (yeast) (Athl1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $833.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pgghg
Synonyms 5730511L01Rik; Athl1; BC023151
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG210060 representing NM_145387
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGGCTCTGAAGATGACCCGACCATATTCAGTGCCCGTTGTCTGCCCAGTGACCCCAGGCTCTGGG
CCACTGTAACGAACTCATACCTAGGTACACGTGTGTATCATGACACCATCCATATAAACGGTGTGTACAA
TGGAGCTGTCGGGGACACTCACCGGGCCAGTCTGCCCAGCCCCCTCAATGTCCAGCTGGAGGCCCCTGCA
GGGACAGAACAGCTGACAGAGACCTTTACCCTGGACACCAACACAGGTTCCTTCCTCCACACCCTGGAAG
GCCCCAGCTTCCGGGCTTCCCAGCGGATCTATGCTCACCGTGTGTTACCCCATGTCCTGGTCTTCAGTGT
GTCCATTGCACGCTTGACCACAGGGAACAAACCCATCACAGTGCCGTTACGGGCAGACTTCTCCCCCGAA
AGCCCAGACCTGGACCTGCGTGTGGGGCCTGACTTCCAGGGACTCCGGTACCTGCATGGCCACGTACTTA
ACCCTGAGCAGCCTGGAGAGCCACAGCAGGAAGTGCATATGCTATGGATGCCTGTCCCCCCAGCCTTGAC
CCTTGGGGAAGAGGAGAAGGACAGGACCTGGGAGTTTCTGACTGTTGTGGGTAGCAGCCAGGCTGAAGCC
CAGGACTGCTTTGCTGAGGCCCTACAGCTGCAGACCAGGGGTGTTCTGTATACAATCCATGCAGACTCCT
GGGGCCGGCTCTGGGCAGGCTGTGGCCTGGATGTGGCTGGGCCCCTGGCCCTGCGCCAGGCCCTTCGTGG
CTCTCTCTACTACCTGTTCAGTGAGCTTCCCCAGCCTGGCACTCAAGGATTCATCAGCCATGGGCTTAGC
CCCGGAGGACTCTCCAATGGGAGTAAGGAGGAATGCTACTGGGGCCATATCTTCTGGGATCAGGACATCT
GGATGTTCCCAAATATTTTGATGTTCCACCCAGAAGCCGCCAGGGCCATCCTAGAATACCGAGTCCGTAC
GCTGGGAGGAGCCCTGAAGAATGGCCAGAACCTGGGCTACCAGGGAGCCAAGTTTGCTTGGGAGAGTGCA
AGCACTGGCCTGGAGGTCTGCCCTGAGGACATTTATGGGACCCAGGAGATCCATATCAATGGGGCTGTGG
CCTTGGCCTTTCAGCTGTATTACTATTACACTCAGGACTCGAAGCTCTTCCAAGAGGATGGTGGCTGGGA
TGTGGTCAGTTCTGTGGCTGAGTTTTGGTGTAGCCGTGTAGAATGGAGCTCCCAGGACAAGATGTACCAC
CTGAAAGGAGTCATGCCCCCCGATGAGTACCATTCAGGAGTCAACAACTCTGTCTACACCAATGTCCTGG
TCCAGAACAGCCTGCACTTCGCTGCTGCTTTGGCCAAGGACTTGGGTTTGCCTATCCGAAAACAGTGGCT
GGAGGTGGCTGATAGGATCAAGATACCATTTGACTCTGAGCAGAACTTCCACCCTGAGTTTGATGGCTAT
GAGCGTGGAGAAGAGGTGAAGCAGGCAGATGTTGTGCTCCTGGGGTACCCAGTCCCCTTTCCCCTGACAC
CTGACATCCGAAGGAAAAACTTGGAGACTTATGAGGCCGTGACATCCCCCCAGGGCCCTGCTATGACCTG
GAGCATGTTTGCTGTGGGCTGGATGGAACTGAGGGACCCCTCAAGGGCTCAGGTCCACCTGAGCAGGAGT
TTCGCCAATGTCACTGAGCCCTTCAAGGTGTGGACGGAGAATGCAGACGGGTCTGGTGCAGTGAACTTCT
TGACTGGCATGGGGGGCTTCCTGCAGGCAGCACTCTTCGGGTGCACAGGATTCAGGATCACTGAGGCAGG
TGTGACCTTTGACCCCCTGTGTCCAGACCTGGTCTCCAGAGTATCTGTCTCTGGTATCTCCTACCTGGGG
AACAAGATCAACTTCGCCTTTTCCAAGGACTCGGTGACACTTGAGGTCACAGCCCGTGCAGAGCCCTGGG
CACCCCTACTAGAAGCTGAGCTGTGGCCATCGCTGGCTCATCTTCCCCTGACACCAGGACAGAAGGTCTC
CTTTCCCCACTCAGCTGGCCGGATACAAAGGTCATCCCCA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG210060 representing NM_145387
Red=Cloning site Green=Tags(s)

MDGSEDDPTIFSARCLPSDPRLWATVTNSYLGTRVYHDTIHINGVYNGAVGDTHRASLPSPLNVQLEAPA
GTEQLTETFTLDTNTGSFLHTLEGPSFRASQRIYAHRVLPHVLVFSVSIARLTTGNKPITVPLRADFSPE
SPDLDLRVGPDFQGLRYLHGHVLNPEQPGEPQQEVHMLWMPVPPALTLGEEEKDRTWEFLTVVGSSQAEA
QDCFAEALQLQTRGVLYTIHADSWGRLWAGCGLDVAGPLALRQALRGSLYYLFSELPQPGTQGFISHGLS
PGGLSNGSKEECYWGHIFWDQDIWMFPNILMFHPEAARAILEYRVRTLGGALKNGQNLGYQGAKFAWESA
STGLEVCPEDIYGTQEIHINGAVALAFQLYYYYTQDSKLFQEDGGWDVVSSVAEFWCSRVEWSSQDKMYH
LKGVMPPDEYHSGVNNSVYTNVLVQNSLHFAAALAKDLGLPIRKQWLEVADRIKIPFDSEQNFHPEFDGY
ERGEEVKQADVVLLGYPVPFPLTPDIRRKNLETYEAVTSPQGPAMTWSMFAVGWMELRDPSRAQVHLSRS
FANVTEPFKVWTENADGSGAVNFLTGMGGFLQAALFGCTGFRITEAGVTFDPLCPDLVSRVSVSGISYLG
NKINFAFSKDSVTLEVTARAEPWAPLLEAELWPSLAHLPLTPGQKVSFPHSAGRIQRSSP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145387
ORF Size 2070 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145387.5
RefSeq Size 2976 bp
RefSeq ORF 2073 bp
Locus ID 212974
UniProt ID Q8BP56
Cytogenetics 7 F5
Summary Catalyzes the hydrolysis of glucose from the disaccharide unit linked to hydroxylysine residues of collagen and collagen-like proteins.UniProtKB/Swiss-Prot Function
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"NM_145387" in other vectors (4)
SKU Description Size Price
MC205081 Athl1 (untagged) - Mouse ATH1, acid trehalase-like 1 (yeast) (Athl1), (10ug) 10 ug
$633.00
MR210060 Athl1 (Myc-DDK-tagged) - Mouse ATH1, acid trehalase-like 1 (yeast) (Athl1) 10 ug
$589.00 $633.00
MR210060L3 Lenti ORF clone of Athl1 (Myc-DDK-tagged) - Mouse ATH1, acid trehalase-like 1 (yeast) (Athl1) 10 ug
$933.00
MR210060L4 Lenti ORF clone of Athl1 (mGFP-tagged) - Mouse ATH1, acid trehalase-like 1 (yeast) (Athl1) 10 ug
$933.00

Plasmid Map for MG210060

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG210060
Circular map for MG210060

file-alt Citations

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