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Cpsf3 (NM_018813) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG210024
Cpsf3 (tGFP-tagged) - Mouse cleavage and polyadenylation specificity factor 3 (Cpsf3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $827.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cpsf3
Synonyms MGC118660
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG210024 representing NM_018813
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGCGATTCCTGCAGAGGAGAGCGACCAGCTGCTGATCCGACCCCTTGGTGCTGGGCAAGAAGTGG
GAAGATCATGTATTATTCTGGAGTTCAAAGGAAGGAAAATCATGCTGGACTGTGGGATCCACCCTGGCCT
GGAAGGAATGGATGCCCTTCCTTACATTGACCTTATCGACCCAGCTGAGATCGACCTTCTGTTGATCAGT
CATTTCCATTTGGATCACTGTGGAGCCCTGCCCTGGTTCCTTCAGAAGACAAGTTTCAAAGGACGGACAT
TTATGACCCACGCCACAAAAGCTATTTATAGATGGCTTCTCTCAGACTATGTCAAAGTCAGCAACATATC
GGCAGATGACATGCTGTACACTGAGACAGACTTGGAAGAAAGCATGGACAAAATCGAAACCATCAATTTT
CATGAAGTTAAGGAAGTTGCAGGAATCAAGTTCTGGTGTTACCATGCCGGCCACGTCCTGGGAGCAGCCA
TGTTTATGATAGAGATCGCCGGTGTGAAGCTTTTGTATACAGGTGATTTCTCAAGACAAGAAGACAGACA
TCTAATGGCTGCTGAGATTCCTAATATTAAGCCAGACATCCTGATCATTGAGTCTACGTATGGGACCCAT
ATCCATGAGAAGCGTGAAGAGCGAGAAGCAAGATTCTGCAACACTGTGCACGACATTGTCAACAGAGGGG
GCAGAGGGCTCATTCCTGTCTTTGCTCTCGGAAGAGCTCAGGAGCTGCTCCTGATTCTAGATGAGTACTG
GCAGAACCACCCGGAGCTGCATGACATACCCATTTACTACGCATCGTCTCTGGCCAAGAAGTGCATGGCC
GTGTACCAGACCTACGTGAATGCCATGAACGACAAGATCCGGAAGCAGATCAACATCAACAACCCCTTTG
TTTTCAAGCACATCAGCAACCTCAAGAGCATGGATCACTTTGATGACATTGGCCCCAGTGTTGTGATGGC
CTCACCAGGCATGATTCAAAACGGCTTATCCAGAGAGCTGTTTGAAAGCTGGTGTACAGATAAGAGGAAT
GGCGTCATCATCGCGGGGTACTGTGTCGAAGGGACACTTGCCAAGCACATCATGTCTGAACCTGAAGAAA
TCACGACCATGTCTGGACAGAAGTTGCCACTGAAAATGTCGGTTGATTACATTTCTTTCTCTGCTCACAC
AGATTACCAGCAAACCAGTGAATTTATTCGTGCTCTGAAGCCACCTCACGTGATTTTAGTCCATGGGGAA
CAGAATGAAATGGCCAGGCTGAAAGCAGCGCTGATCCGAGAATATGAAGATAATGATGAAGTTCACATCG
AGGTTCACAATCCTCGGAACACAGAGGCCGTGACCTTAAACTTCAGGGGAGAGAAGCTCGCCAAGGTCAT
GGGCTTTTTGGCAGACAAGAAACCAGAACAGGGCCAGCGGGTCTCTGGAATTCTTGTTAAAAGAAACTTT
AATTACCATATACTCTCTCCCTGTGACCTCTCCAATTACACTGACCTGGCCATGAGCACTGTGAAACAGA
CCCAGGCCATCCCGTACACCGGGCCCTTTTACCTGCTCTACTACCAGCTCCAGAAATTGACGGGTGATGT
AGAAGAATTAGAAATTCAAGAAAAGCCTGCTCTGAAGGTGTTCAAAAGCATCACTGTGGTGCAGGAGCCG
GGCATGGTGGTTCTGGAGTGGCTGGCGAACCCCTCTAACGACATGTATGCAGACACAGTGACCACCGTGA
TATTGGAAGTTCAGTCAAATCCAAAGATAAGGAAAGGTGCAGTACAGAAGGTCTCTAAGAAGCTAGAGAT
GCACGTTTACAGCAAGAGGCTGGAGGTCATGCTCCAGGACATATTTGGAGAAGACTGTGTCAGTGTGAAA
GATGACTCCGTCCTCAGTGTCACAGTGGACGGGAAAACAGCAAATATTAATCTGGAGACTCGGGCTGTAG
AATGTGAAGAAGGAAGTGAAGATGATGAATCCCTCCGAGAAATGGTGGAACTGGCCGCCCAGAGACTGTA
CGAGGCTCTGACGCCGGTGCAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG210024 representing NM_018813
Red=Cloning site Green=Tags(s)

MSAIPAEESDQLLIRPLGAGQEVGRSCIILEFKGRKIMLDCGIHPGLEGMDALPYIDLIDPAEIDLLLIS
HFHLDHCGALPWFLQKTSFKGRTFMTHATKAIYRWLLSDYVKVSNISADDMLYTETDLEESMDKIETINF
HEVKEVAGIKFWCYHAGHVLGAAMFMIEIAGVKLLYTGDFSRQEDRHLMAAEIPNIKPDILIIESTYGTH
IHEKREEREARFCNTVHDIVNRGGRGLIPVFALGRAQELLLILDEYWQNHPELHDIPIYYASSLAKKCMA
VYQTYVNAMNDKIRKQININNPFVFKHISNLKSMDHFDDIGPSVVMASPGMIQNGLSRELFESWCTDKRN
GVIIAGYCVEGTLAKHIMSEPEEITTMSGQKLPLKMSVDYISFSAHTDYQQTSEFIRALKPPHVILVHGE
QNEMARLKAALIREYEDNDEVHIEVHNPRNTEAVTLNFRGEKLAKVMGFLADKKPEQGQRVSGILVKRNF
NYHILSPCDLSNYTDLAMSTVKQTQAIPYTGPFYLLYYQLQKLTGDVEELEIQEKPALKVFKSITVVQEP
GMVVLEWLANPSNDMYADTVTTVILEVQSNPKIRKGAVQKVSKKLEMHVYSKRLEVMLQDIFGEDCVSVK
DDSVLSVTVDGKTANINLETRAVECEEGSEDDESLREMVELAAQRLYEALTPVH

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018813
ORF Size 2052 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018813.2, NP_061283.2
RefSeq Size 2253 bp
RefSeq ORF 2055 bp
Locus ID 54451
UniProt ID Q9QXK7
Cytogenetics 12 A1.3
Summary Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP-dependent protein that induces both the 3' endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_018813" in other vectors (4)
SKU Description Size Price
MC202882 Cpsf3 (untagged) - Mouse cleavage and polyadenylation specificity factor 3 (Cpsf3), (10ug) 10 ug
$627.00
MR210024 Cpsf3 (Myc-DDK-tagged) - Mouse cleavage and polyadenylation specificity factor 3 (Cpsf3) 10 ug
$589.00 $627.00
MR210024L3 Lenti ORF clone of Cpsf3 (Myc-DDK-tagged) - Mouse cleavage and polyadenylation specificity factor 3 (Cpsf3) 10 ug
$927.00
MR210024L4 Lenti ORF clone of Cpsf3 (mGFP-tagged) - Mouse cleavage and polyadenylation specificity factor 3 (Cpsf3) 10 ug
$927.00

Plasmid Map for MG210024

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MG210024
Circular map for MG210024

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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