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Cast (BC010603) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG209946
Cast (tGFP-tagged) - Mouse calpastatin (cDNA clone MGC:12116 IMAGE:3710078)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
$789.00 $817.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cast
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG209946 representing BC010603
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTACCACAGAGACTAAGGCTGTGAAAACAGAGTCTAAGAAACCACAATCAAGTGAGCAACCTGTGG
TTCACGAGAAAAAGTCAAAAGGAGGGCCAAAGGAAGGGTCAGAGCCCAAAAATCTTCCGAAGCACACATC
AAGTACAGGAAGCAAGCATGCTCATAAAGAAAAAGCACTTTCCAGATCAAATGAACAGATGGTGTCAGAG
AAACCATCAGAATCAAAGACAAAATTTCAAGATGTTCCTTCAGCTGGTGGAGAGAGTGTGGCTGGAGGTG
GGACTGTGGCCACTGCCTTGGACAAAGTGGTTGGTAAGAAAAAAGAACAGAAACCATTCACACCAGCTTC
ACCTGTGCAGTCCACACCGAGCAAGCCATCTGACAAGTCAGGCATGGATGCTGCCTTGGATGACCTGATA
GACACCTTAGGTGGACATGAAGATACTAACAGAGATGATCCACCATATACTGGACCAGTAGTTTTGGACC
CAATGTATTCTACCTACCTTGAGGCACTGGGTATAAAAGAAGGGACTATTCCTCCAGAGTATAGGAAACT
TTTGGAAAAAAATGAAGGGATCACACAACCTCTTCCAGACTCTCCTAAACCTATGGGGACTGACCAGGCT
ATAGATGCCTTGTCATCTGATTTCACCTGTAGTTCTCCAACTGGAAAGCAAAGTGAGAAAGAGAAGTCTA
CTGGGGAAATTTTCAAAGCTCAGTCTGCAGGAGTGACCAGAAGTTCTGTTCCCCCCAAGGAGAAGAAAAG
GAAGGTGGAAGAGGAGGTCATAAATGATCAAGCACTTCAGGCTCTGTCAGATTCACTGGGCACCCGGCAG
CCAGATCCTCCGAGCCACGTTAGCCAAGCTGAACAAGTCAAAGAGGCAAAAGCAAAAGAAGAAAGGCAGG
AGAAGTGTGGTGAAGATGAGGACACAGTCCCAGCTGAGTACAGGTTAAAACCAGCAAAGGATAAAGATGG
AAAACCACTATTGCCAGAGCCTGAAGAAACATCTAAGAGCCTGAGTGAGTCGGAGCTGATTGGGGAGCTT
TCAGCAGATTTTGACCGATCTACATATCAAGACAAACCATCTACGCCAGCTGAAAAGAAATCCAATGACA
CATCCCAAACTCCTCCGGGGGAGACTGTGCCTCGGGCCTCCATGTGCAGTATACGGTCAGCGCCACCCAA
ACTAGCATCCTTGAAGGGCGTGGTACCAGAAGATGCTGTTGAAACCTTGGCTGGAAGCCTGGGGACAAGG
GAAGCAGATCCAGAACATGAAAAAACTGTGGAGGATAAAGTCAAGGAGAAAGCTAAAGAAGAAGAGCATG
AAAAACTTGGTGAAAAAGAAGAAACAGTGCCTCCTGATTATCGACTAGAAGAAGTCAAGGATAAGGATGG
AAAACCACTCCTGCCCAAAGAATCCCAGGAACAACTTGCACCCTTAAGCGATGACTTCCTTCTTGATGCC
TTGTCTCAGGACTTCTCCAGTCCGGCAAACATATCGTCTCTTGAATTTGAAGATGCCAAACTTTCTGCTG
CCATTTCTGAAGTAGTTTCTCAGACACCTGCTCCAAGCACCCATGCAGCAGCTCCACTGCCTGGCACTGA
GCAGAAAGACAAAGAACTTGATGATGCCTTGGATGAACTTTCTGACAGTCTTGGACAAAGGCCGCCTGAT
CCAGATGAGAACAAACCACTGGATGACAAAGTGAAGGAGAAAATCAAACCAGAGCATAGCGAGAAACTGG
GAGAAAGAGACGACACCATCCCCCCTGAATACAGGCATCTCTTGGATAATGATGGGAAGAAACCTGATCA
GGACCGGGACCCCATTGATGCCCTCTCAGAAGATTTGGATAGCTGCCCCTCAACTACAGAGACCTCAAAG
AATACAGCAAAGGGGAAGAGCAAGAAGACTTCAAGTTCCAAAGCATCCAAGGACGGAGAGAAAACAAAGG
ACTCTTCCAAGAAGACAGAGGAAGTGTCCAAGCCAAAGGCTAAAGAAGATGCAAGACACAGT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG209946 representing BC010603
Red=Cloning site Green=Tags(s)

MSTTETKAVKTESKKPQSSEQPVVHEKKSKGGPKEGSEPKNLPKHTSSTGSKHAHKEKALSRSNEQMVSE
KPSESKTKFQDVPSAGGESVAGGGTVATALDKVVGKKKEQKPFTPASPVQSTPSKPSDKSGMDAALDDLI
DTLGGHEDTNRDDPPYTGPVVLDPMYSTYLEALGIKEGTIPPEYRKLLEKNEGITQPLPDSPKPMGTDQA
IDALSSDFTCSSPTGKQSEKEKSTGEIFKAQSAGVTRSSVPPKEKKRKVEEEVINDQALQALSDSLGTRQ
PDPPSHVSQAEQVKEAKAKEERQEKCGEDEDTVPAEYRLKPAKDKDGKPLLPEPEETSKSLSESELIGEL
SADFDRSTYQDKPSTPAEKKSNDTSQTPPGETVPRASMCSIRSAPPKLASLKGVVPEDAVETLAGSLGTR
EADPEHEKTVEDKVKEKAKEEEHEKLGEKEETVPPDYRLEEVKDKDGKPLLPKESQEQLAPLSDDFLLDA
LSQDFSSPANISSLEFEDAKLSAAISEVVSQTPAPSTHAAAPLPGTEQKDKELDDALDELSDSLGQRPPD
PDENKPLDDKVKEKIKPEHSEKLGERDDTIPPEYRHLLDNDGKKPDQDRDPIDALSEDLDSCPSTTETSK
NTAKGKSKKTSSSKASKDGEKTKDSSKKTEEVSKPKAKEDARHS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC010603
ORF Size 2022 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC010603, AAH10603
RefSeq Size 2712 bp
RefSeq ORF 2024 bp
Locus ID 12380
Cytogenetics 13 C1
Summary This gene encodes an inhibitor of the calcium-dependent cysteine protease, calpain. This protein plays roles in multiple processes, including apoptosis, cell cycle regulation, and membrane fusion. Multiple protein isoforms exist which contain unique N-terminal domains, and multiple inhibitory domains that share homology with each other. Some isoforms may be tissue-specific. Two different pseudogenes of this gene are found on chromosome 19. provided by RefSeq, Jul 2014
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Documents
Resources
"BC010603" in other vectors (4)
SKU Description Size Price
MC200735 Cast (untagged) - Mouse calpastatin (cDNA clone MGC:12116 IMAGE:3710078), (10ug) 10 ug
$618.00
MR209946 Cast (Myc-DDK-tagged) - Mouse calpastatin (cDNA clone MGC:12116 IMAGE:3710078) 10 ug
$589.00 $617.00
MR209946L3 Lenti ORF clone of Cast (Myc-DDK-tagged) - Mouse calpastatin (cDNA clone MGC:12116 IMAGE:3710078) 10 ug
$917.00
MR209946L4 Lenti ORF clone of Cast (mGFP-tagged) - Mouse calpastatin (cDNA clone MGC:12116 IMAGE:3710078) 10 ug
$917.00

Plasmid Map for MG209946

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG209946
Circular map for MG209946

file-alt Citations

Calpains are downstream effectors of bax-dependent excitotoxic apoptosis
D'Orsi, Bonner, Tuffy et al
J. Neurosci. (2012) 32 (5), 1847-58 DOI: 10.1523/JNEUROSCI.2345-11.2012
Cited by 10
AI Summary: OriGene’s GFP–calpastatin expression vector (MG209946) was used to overexpress calpastatin in neurons, enabling the authors to assess the protective effect of calpain inhibition during excitotoxic apoptosis.
Snippet: For overexpression of calpastatin, cells were transfected with a vector expressing GFP–calpastatin (MG209946;OriGene). ... TMRM was excited at 543 nm, and the emission was collected with a 560 nm long-pass filter;Fluo-4 AM was excited at 488 nm, and the emission was
Characterisation of calpain activation in response to excitotoxic events in primary neurons
D'Orsi
Thesis (2012)
Snippet: The Green Fluorescent Protein (GFP)-calpastatin vector (MG209946, ORIGENE, Cambridge, UK) was used for calpastatin overexpression experiments
Showing 1-2 of 2 papers.
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