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Tcf3 (NM_011548) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG209745
Tcf3 (tGFP-tagged) - Mouse transcription factor E2a (Tcfe2a)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
$489.00 $803.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tcf3
Synonyms A1; AA408400; ALF2; AW209082; bHLHb21; E2A; E12; E12/E47; E47; KA1; ME2; Pan1; Pan2; TCF-3; Tcfe2a
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011548
ORF Size 1944 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011548.3, NP_035678.2
RefSeq Size 3305 bp
RefSeq ORF 1947 bp
Locus ID 21423
UniProt ID P15806
Cytogenetics 10 39.72 cM
Summary Transcriptional regulator. Involved in the initiation of neuronal differentiation. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region.UniProtKB/Swiss-Prot Function
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"NM_011548" in other vectors (4)
SKU Description Size Price
MC220059 Tcf3 (untagged) - Mouse transcription factor 3 (Tcf3), transcript variant 8, (10ug) 10 ug
$664.00
MR209745 Tcf3 (Myc-DDK-tagged) - Mouse transcription factor 3 (Tcf3), transcript variant 8 10 ug
$289.00 $603.00
MR209745L3 Lenti ORF clone of Tcf3 (Myc-DDK-tagged) - Mouse transcription factor 3 (Tcf3), transcript variant 8 10 ug
$903.00
MR209745L4 Lenti ORF clone of Tcf3 (mGFP-tagged) - Mouse transcription factor 3 (Tcf3), transcript variant 8 10 ug
$903.00

Plasmid Map for MG209745

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Cloning scheme for MG209745
Circular map for MG209745

file-alt Citations

Inflammation-induced Id2 promotes plasticity in regulatory T cells
Hwang, Sharma, Verma et al
Nat Commun (2018) 9 (1), 4736 DOI: 10.1038/s41467-018-07254-2
AI Summary: OriGene products—STAT3 (MR227265), IRF4 (MR226642), BATF (MR222114), E2A (MG209745), and Id2 (MR200792) expression plasmids—were used in co-transfection experiments to investigate the regulatory mechanisms controlling Id2 and Foxp3 promoter activity in T cell plasticity.
Snippet: (Lonza) according to the manufacturer’s protocol and plated in 12 well plate. 0.5 μg of the _Id2_ promoter-luciferase constructs or pGL4.17 empty vector was co-transfected with STAT3 (MR227265;Origene), IRF4 (MR226642;Origene) and BATF (MR222114;Origene) expression plasmids (HEK-293 T) and 1 μg of the _Foxp3_ promoter-luciferase construct was co-transfected with E2A (MG209745;Origene);HEK-293 T cells or Jurkat cells were transfected using FuGENE HD (Promega) or NucleofectorTM (Lonza) according to the manufacturer’s protocol and plated in 12 well plate. 0.5 μg of the Id2 promoter-luciferase constructs or pGL4.17 empty vector was co-transfected with STAT3 (MR227265;Origene) expression plasmids (HEK-293 T) and 1 μg of the Foxp3 promoter-luciferase construct was co-transfected with E2A (MG209745;Origene) and Id2 (MR200792;Origene) expression plasmids (Jurkat). …
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Showing 1-1 of 1 papers.
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