Ash2l (NM_011791) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG209616
Ash2l (tGFP-tagged) - Mouse ash2 (absent, small, or homeotic)-like (Drosophila) (Ash2l), transcript variant 1
  $1,071.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ash2l
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG209616 representing NM_011791
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGGCTGGAGCGGGTCCTGGTCCGGGAGTCAGCGCCGGCCCTGGTCCCGGAGCCGCTGCAAGTG
CCACAACGGCGGAGGACCGAGAGACAGAGCCGGTGGCCGCGGGGGCTGGCGAGGGGCCGTCAGCCGCCCC
GGGAGCGGAGCCCAGCTCCGGAGAGGCCGAAAGTGGGGATGCAAACTTGGTTGATGTAAGTGGTCTGGAG
ACAGAATCTTCTAATGGAAAAGATACTTTGGAAGGTACCGGAGACACTTCAGAAGTGATGGATACCCAGG
CGGGCTCTGTGGATGAGGAGAATGGGCGGCAGTTGGGAGAAGTGGAGTTGCAGTGTGGGATATGTACAAA
ATGGTTCACCGCTGACACCTTTGGAATAGACACGTCGTCATGTCTGCCTTTTATGACCAACTACAGCTTC
CATTGCAATGTGTGCCATCACAGTGGGAATACCTACTTTCTCCGGAAGCAAGCAAATTTAAAAGAAATGT
GCCTTAGTGCCTTGGCGAATTTGACGTGGCAGTCTCGAACACAGGATGAACATCCAAAAACCATGTTCTC
CAAAGATAAGGATATTATACCATTTATTGATAAATACTGGGAATGTATGACAACCAGACAGAGACCTGGA
AAAATGACTTGGCCCAATAACATTGTCAAAACAATGAGTAAGGAAAGAGATGTGTTCTTGGTAAAGGAAC
ACCCTGACCCAGGAAGCAAAGACCCAGAAGAAGATTACCCCAAGTTTGGACTTTTGGATCAGGATCTTAG
TAATATTGGTCCTGCTTATGACAACCAGAAACAAAGCAGTGCTGTGTCTGCTAGTGGGAACCTAAATGGG
GGGATCGCAGCCGGAAGCAGTGGGAAAGGAAGAGGAGCCAAGCGTAAGCAGCAAGATGGAGGGACAACAG
GGACCACCAAGAAGGCCAGAAGTGATCCTTTATTTTCTGCTCAGCGTCTCCCTCCTCATGGCTATCCTTT
GGAACATCCATTTAACAAAGATGGCTATCGGTATATTCTTGCTGAGCCCGATCCTCATGCCCCCGACCCG
GAGAAGCTTGAACTTGACTGCTGGGCGGGAAAGCCTATTCCTGGAGACCTTTACAGAGCCTGCTTATATG
AACGAGTCTTGTTAGCCCTACATGATCGAGCTCCCCAGTTAAAGATCTCTGATGACCGGCTGACCGTGGT
TGGAGAGAAGGGCTACTCCATGGTCCGGGCCTCTCATGGGGTACGCAAAGGGGCCTGGTACTTTGAAATC
ACTGTGGATGAGATGCCCCCAGACACTGCTGCCAGGCTGGGCTGGTCCCAGCCCTTAGGTAACCTCCAAG
CTCCCTTAGGCTATGATAAGTTTAGCTATTCTTGGCGGAGCAAGAAAGGCACCAAGTTCCACCAGTCCAT
TGGCAAGCACTATTCGTCTGGCTACGGACAAGGGGACGTCCTAGGGTTCTATATCAACCTTCCCGAAGAC
ACAGAGACAGCCAAGTCACTGCCGGACACCTACAAAGATAAGGCTTTGATAAAGTTCAAGAGTTATTTGT
ATTTTGAAGAAAAAGACTTTGTGGACAAAGCAGAGAAGAGCCTAAAACAGACCCCCCATAGTGAGATAAT
ATTTTATAAAAATGGTGTCAATCAGGGTGTGGCTTACAGAGATATTTTTGAAGGAGTTTACTTCCCAGCC
ATCTCACTGTACAAGAGCTGCACGGTTTCCATTAACTTTGGACCATCCTTCAAATACCCTCCAAAGGATC
TCACGTACCACCCTATGAGTGACATGGGCTGGGGCGCTGTGGTAGAACACACACTGGCTGATGTTTTGTA
TCACGTGGAGACAGAAGTGGACGGAAGACGTAGTCCACCCTGGGAACCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG209616 representing NM_011791
Red=Cloning site Green=Tags(s)

MAAAGAGPGPGVSAGPGPGAAASATTAEDRETEPVAAGAGEGPSAAPGAEPSSGEAESGDANLVDVSGLE
TESSNGKDTLEGTGDTSEVMDTQAGSVDEENGRQLGEVELQCGICTKWFTADTFGIDTSSCLPFMTNYSF
HCNVCHHSGNTYFLRKQANLKEMCLSALANLTWQSRTQDEHPKTMFSKDKDIIPFIDKYWECMTTRQRPG
KMTWPNNIVKTMSKERDVFLVKEHPDPGSKDPEEDYPKFGLLDQDLSNIGPAYDNQKQSSAVSASGNLNG
GIAAGSSGKGRGAKRKQQDGGTTGTTKKARSDPLFSAQRLPPHGYPLEHPFNKDGYRYILAEPDPHAPDP
EKLELDCWAGKPIPGDLYRACLYERVLLALHDRAPQLKISDDRLTVVGEKGYSMVRASHGVRKGAWYFEI
TVDEMPPDTAARLGWSQPLGNLQAPLGYDKFSYSWRSKKGTKFHQSIGKHYSSGYGQGDVLGFYINLPED
TETAKSLPDTYKDKALIKFKSYLYFEEKDFVDKAEKSLKQTPHSEIIFYKNGVNQGVAYRDIFEGVYFPA
ISLYKSCTVSINFGPSFKYPPKDLTYHPMSDMGWGAVVEHTLADVLYHVETEVDGRRSPPWEP

TRTRPLE - GFP Tag - V
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011791
ORF Size 1869 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011791.3
RefSeq Size 2427 bp
RefSeq ORF 1872 bp
Locus ID 23808
UniProt ID Q91X20
Cytogenetics 8 A2
Summary Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_011791" in other vectors (4)
SKU Description Size Price
MC200805 Ash2l (untagged) - Mouse ash2 (absent, small, or homeotic)-like (Drosophila) (Ash2l), transcript variant 1, (10ug) 10 ug
$871.00
MR222173 Ash2l (Myc-DDK-tagged) - Mouse ash2 (absent, small, or homeotic)-like (Drosophila) (Ash2l), transcript variant 1 10 ug
$928.00
MR222173L3 Lenti ORF clone of Ash2l (Myc-DDK-tagged) - Mouse ash2 (absent, small, or homeotic)-like (Drosophila) (Ash2l), transcript variant 1 10 ug
$1,228.00
MR222173L4 Lenti ORF clone of Ash2l (mGFP-tagged) - Mouse ash2 (absent, small, or homeotic)-like (Drosophila) (Ash2l), transcript variant 1 10 ug
$1,228.00

Plasmid Map for MG209616

Download sequence for

Cloning scheme for MG209616

Circular map for MG209616

file-alt Citations

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