Kcnd2 (NM_019697) Mouse Tagged ORF Clone
- TrueORF®
SKU
MG209597
Kcnd2 (tGFP-tagged) - Mouse potassium voltage-gated channel, Shal-related family, member 2 (Kcnd2)
$1,081.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Kcnd2 |
| Synonyms | AI839615; AW555701; Kv4.2; mKIAA1044; R75121 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_019697 |
| ORF Size | 1890 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_019697.4 |
| RefSeq Size | 4562 bp |
| RefSeq ORF | 1893 bp |
| Locus ID | 16508 |
| UniProt ID | Q9Z0V2 |
| Cytogenetics | 6 8.49 cM |
| Summary | Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes, primarily in the brain, but also in rodent heart. Mediates the major part of the dendritic A-type current I(SA) in brain neurons (PubMed:10818150, PubMed:17122039, PubMed:18045912, PubMed:18187474, PubMed:20371829, PubMed:22815518). This current is activated at membrane potentials that are below the threshold for action potentials. It regulates neuronal excitability, prolongs the latency before the first spike in a series of action potentials, regulates the frequency of repetitive action potential firing, shortens the duration of action potentials and regulates the back-propagation of action potentials from the neuronal cell body to the dendrites (PubMed:10818150, PubMed:17122039, PubMed:22815518). Contributes to the regulation of the circadian rhythm of action potential firing in suprachiasmatic nucleus neurons, which regulates the circadian rhythm of locomotor activity (PubMed:22815518). Functions downstream of the metabotropic glutamate receptor GRM5 and plays a role in neuronal excitability and in nociception mediated by activation of GRM5 (PubMed:18045912). Mediates the transient outward current I(to) in rodent heart left ventricle apex cells, but not in human heart, where this current is mediated by another family member (PubMed:9734479, PubMed:10601491, PubMed:11909823, PubMed:23713033). Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane (PubMed:9734479, PubMed:22311982). Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCND2 and KCND3; channel properties depend on the type of pore-forming alpha subunits that are part of the channel (PubMed:11909823). In vivo, membranes probably contain a mixture of heteromeric potassium channel complexes (PubMed:11909823). Interaction with specific isoforms of the regulatory subunits KCNIP1, KCNIP2, KCNIP3 or KCNIP4 strongly increases expression at the cell surface and thereby increases channel activity; it modulates the kinetics of channel activation and inactivation, shifts the threshold for channel activation to more negative voltage values, shifts the threshold for inactivation to less negative voltages and accelerates recovery after inactivation (By similarity). Likewise, interaction with DPP6 or DPP10 promotes expression at the cell membrane and regulates both channel characteristics and activity (PubMed:22311982).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_019697" in other vectors (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MR209597 | Kcnd2 (Myc-DDK-tagged) - Mouse potassium voltage-gated channel, Shal-related family, member 2 (Kcnd2) | 10 ug |
$881.00
|
|
| MR209597L3 | Lenti ORF clone of Kcnd2 (Myc-DDK-tagged) - Mouse potassium voltage-gated channel, Shal-related family, member 2 (Kcnd2) | 10 ug |
$1,181.00
|
|
| MR209597L4 | Lenti ORF clone of Kcnd2 (mGFP-tagged) - Mouse potassium voltage-gated channel, Shal-related family, member 2 (Kcnd2) | 10 ug |
$1,181.00
|
|
Citations
Kv4.1, a Key Ion Channel For Low Frequency Firing of Dentate Granule Cells, Is Crucial for Pattern Separation
Kim, Lee, Yoon et al
J Neurosci (2020) 40 (11), 2200-2214 DOI: 10.1523/JNEUROSCI.1541-19.2020
Kim, Lee, Yoon et al
J Neurosci (2020) 40 (11), 2200-2214 DOI: 10.1523/JNEUROSCI.1541-19.2020
AI Summary: In this study, OriGene products—including the anti-Kv4.1 antibody (Cat# TA326467) and Kv4.1 shRNA plasmid (Cat# TL513627)—were used to selectively inhibit Kv4.1 channel function and knock down Kv4.1 expression, enabling the authors to demonstrate Kv4.1’s critical role in regulating firing frequency of dentate granule cells and its importance for pattern separation in the hippocampus.
Kv4. 1, a key ion channel for low frequency firing of dentate granule cells, is crucial for pattern separation
Kim, Lee, Kim et al
bioRxiv (2019)
Kim, Lee, Kim et al
bioRxiv (2019)
Showing 1-3 of 3 papers.
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