Pmel (NM_021882) Mouse Tagged ORF Clone

SKU
MG209549
Pmel (tGFP-tagged) - Mouse silver (Si)
  • TrueORF®
    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

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$489.00 MSRP $784.00 MSRP $784.00
In Stock*
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pmel
Synonyms D10H12S53E; D12S53Eh; gp87; gp100; Pmel17; Si; Silv
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG209549 representing NM_021882
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTGTCCAGAGAAGGAGCTTCCTTCCCGTGCTTGTGCTGAGTGCTCTGCTGGCTGTGGGGGCCCTAG
AAGGATCCAGGAATCAGGACTGGCTTGGTGTCCCAAGACAACTTGTAACTAAAACCTGGAACAGGCAGCT
GTACCCCGAGTGGACAGAGGTGCAGGGGTCTAACTGCTGGAGAGGTGGCCAGGTATCTCTGAGGGTCATT
AATGATGGGCCTACACTGGTTGGTGCAAATGCCTCCTTTTCCATTGCCCTGCACTTCCCTGGAAGTCAAA
AGGTACTACCGGATGGTCAGGTTATCTGGGCCAACAACACCATCATCAATGGGAGCCAGGTGTGGGGAGG
ACAGCCAGTGTATCCACAGGAGCCTGATGATGCCTGTGTCTTCCCTGACGGTGGACCCTGCCCATCTGGT
CCTAAACCTCCGAAGAGAAGCTTTGTTTATGTTTGGAAGACCTGGGGAAAATACTGGCAAGTTCTGGGGG
GTCCAGTGTCCAGGCTGAGCATTGCTACGGGCCACGCAAAGCTGGGCACACACACAATGGAAGTGACTGT
CTACCACCGACGGGGTTCCCAGAGCTACGTGCCCCTTGCTCACGCCAGTTCAACCTTCACCATTACTGAC
CAGGTACCTTTCTCCGTGAGTGTGTCCCAGCTACAGGCCTTGGACGGAGAGACCAAGCACTTCCTGAGAA
ATCATCCTCTCATCTTTGCCCTTCAGCTCCACGACCCCAGTGGTTATTTGGCCGAGGCCGACCTCTCCTA
CACATGGGACTTTGGAGATGGTACTGGGACCCTGATCTCTCGGGCACTTGATGTCACTCACACTTACCTG
GAGTCGGGCTCAGTCACTGCCCAGGTGGTACTGCAGGCTGCCATTCCTCTTGTTTCCTGTGGTTCCTCCC
CAGTCCCGGGTACCACAGATGGCTACATGCCAACTGCAGAAGCACCTGGAACCACATCTAGGCAAGGAAC
CACTACAAAAGTTGTGGGTACTACACCTGGCCAGATGCCAACTACACAGCCCTCTGGAACCACAGTTGTA
CAAATGCCAACCACAGAGGTCACAGCTACTACATCTGAGCAGATGCTGACCTCAGCGGTCATAGATACCA
CACTGGCAGAGGTGTCAACTACAGAGGGTACAGGTACCACACCCACAAGGCCTTCTGGAACCACCGTTGC
ACAAGCAACAACCACAGAGGGTCCAGATGCCAGCCCATTGCTGCCCACACAAAGTTCTACAGGGTCCATT
AGCCCTCTACTGGATGACACCGACACCATAATGCTTGTGAAGAGACAAGTTCCCCTGGACTGTGTTCTAT
ATCGATATGGTTCTTTCTCCCTCGCCCTGGACATTGTCCAGGGTATTGAAAGTGCTGAGATCCTGCAGGC
TGTGCCATTCAGTGAAGGGGATGCATTTGAGCTGACTGTGTCCTGCCAAGGCGGGCTACCCAAGGAAGCC
TGTATGGACATTTCATCACCAGGGTGCCAGCCCCCTGCCCAGAGGCTGTGCCAGTCTGTTCCACCGAGCC
CAGACTGCCAGCTGGTTCTACACCAAGTGCTGAAAGGTGGCTCAGGGACATATTGCCTCAATGTGTCTTT
GGCTGACGCCAACAGCCTGGCAGTGGCCAGCACCCAACTTGTTGTTCCTGGTCAAGACGGTGGCCTTGGG
CAGGCTCCCTTGCTTGTAGGTATCTTGCTGGTGTTGGTGGCTGTGGTCCTTGCATCTCTGATACATAGGC
ATAGACTTAAGAAGCAGGGCTCAGTTTCCCAAATGCCACATGGTAGCACTCACTGGCTGCGCCTGCCTCC
GGTCTTCCGCGCCCGCGGCCTTGGAGAAAACAGCCCGCTCCTCAGTGGACAGCAGGTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG209549 representing NM_021882
Red=Cloning site Green=Tags(s)

MGVQRRSFLPVLVLSALLAVGALEGSRNQDWLGVPRQLVTKTWNRQLYPEWTEVQGSNCWRGGQVSLRVI
NDGPTLVGANASFSIALHFPGSQKVLPDGQVIWANNTIINGSQVWGGQPVYPQEPDDACVFPDGGPCPSG
PKPPKRSFVYVWKTWGKYWQVLGGPVSRLSIATGHAKLGTHTMEVTVYHRRGSQSYVPLAHASSTFTITD
QVPFSVSVSQLQALDGETKHFLRNHPLIFALQLHDPSGYLAEADLSYTWDFGDGTGTLISRALDVTHTYL
ESGSVTAQVVLQAAIPLVSCGSSPVPGTTDGYMPTAEAPGTTSRQGTTTKVVGTTPGQMPTTQPSGTTVV
QMPTTEVTATTSEQMLTSAVIDTTLAEVSTTEGTGTTPTRPSGTTVAQATTTEGPDASPLLPTQSSTGSI
SPLLDDTDTIMLVKRQVPLDCVLYRYGSFSLALDIVQGIESAEILQAVPFSEGDAFELTVSCQGGLPKEA
CMDISSPGCQPPAQRLCQSVPPSPDCQLVLHQVLKGGSGTYCLNVSLADANSLAVASTQLVVPGQDGGLG
QAPLLVGILLVLVAVVLASLIHRHRLKKQGSVSQMPHGSTHWLRLPPVFRARGLGENSPLLSGQQV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_021882
ORF Size 1878 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021882.4
RefSeq Size 2132 bp
RefSeq ORF 1881 bp
Locus ID 20431
Cytogenetics 10 77.13 cM
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MR209549L2 Lenti ORF clone of Pmel (mGFP-tagged) - Mouse premelanosome protein (Pmel) 10 ug
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MR209549L3 Lenti ORF clone of Pmel (Myc-DDK-tagged) - Mouse premelanosome protein (Pmel) 10 ug
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Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.