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Atg16l1 (BC049122) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG209513
Atg16l1 (tGFP-tagged) - Mouse autophagy-related 16-like 1 (yeast) (cDNA clone MGC:61241 IMAGE:6813377)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
$489.00 $780.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Atg16l1
Synonyms WDR30, Atg16l
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG209513 representing BC049122
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGTCGGGCCTGCGCGCCGCAGACTTTCCCCGCTGGAAGCGTCACATCGCGGAGGAACTGAGGCGCC
GGGACCGACTGCAGAGGCAGGCGTTCGAGGAGATCATTCTGCAGTATACCAAGTTGCTGGAAAAGTCAGA
TCTTCATTCAGTATTGACCCAGAAACTACAAGCAGAAAAGCATGACATGCCAAATAGGCATGAAATAAGT
CCTGGACATGATGGTGCGTGGAATGATAGTCAACTACAAGAAATGGCCCAGTTGAGGATCAAACACCAGG
AAGAGCTGACCGAACTGCACAAGAAGCGTGGGGAGTTAGCTCAGTTGGTGATTGACCTGAACAACCAAAT
GCAGCAGAAGGACAAGGAGATACAGATGAATGAAGCAAAGATTTCGGAGTATTTACAGACCATCTCTGAC
CTGGAGACAAACTGCCTGGACCTGCGCACCAAACTGCAGGACCTCGAGGTAGCCAACCAGACCCTGAAGG
ATGAGTATGACGCCCTGCAGATTACTTTTACTGCCCTAGAAGAGAAACTGAGGAAAACTACTGAGGAGAA
CCAGGAACTGGTCACCAGATGGATGGCTGAGAAGGCCCAAGAAGCCAATCGCCTCAATGCAGAGAATGAG
AAGGACTCCAGGAGGCGTCAAGCACGGCTGCAGAAGGAGCTTGCAGAAGCAGCAAAGGAACCTCTACCTG
TTGAACAGGATGATGACATTGAAGTCATTGTGGATGAGACCTCAGACCACACAGAAGAGACCTCTCCCGT
CCGAGCTGTCAGCAGAGCAGCTACTAAGCGACTCTCGCAGCCTGCTGGAGGCCTTCTGGATTCTATCACT
AATATCTTTGGTCTGTCCGAATCTCCCCTTTTGGGACATCATTCTTCTGATGCTGCCAGGAGACGCTCTG
TCTCTTCCATCCCAGTCCCCCAGGATATCATGGACACTCATCCTGCTTCTGGTAAAGATGTGAGAGTCCC
AACTACTGCCTCGTATGTCTTCGATGCGCATGACGGAGAGGTCAACGCAGTGCAGTTCAGTCCAGGCTCC
CGGTTGCTGGCCACTGGAGGCATGGACCGCAGGGTGAAACTTTGGGAAGCATTCGGAGATAAATGTGAAT
TCAAGGGCTCCCTGTCTGGCAGTAATGCTGGAATTACAAGCATTGAATTTGATAGTGCTGGAGCTTACCT
ATTAGCAGCTTCAAATGATTTTGCAAGCCGAATCTGGACTGTGGATGATTATCGATTACGGCACACACTC
ACAGGCCACAGCGGGAAAGTCCTCTCTGCCAAGTTCCTGCTGGACAATGCACGGATTGTCTCAGGAAGTC
ACGACCGGACCCTCAAACTCTGGGATCTCCGCAGCAAAGTCTGCATAAAAACAGTGTTTGCAGGATCCAG
CTGCAATGACATTGTTTGCACTGAACAATGTGTAATGAGTGGACATTTTGACAAGAAAATTCGTTTCTGG
GATATCCGGTCAGAGAGTGTGGTCCGAGAGATGGAACTGTTAGGGAAGATCACTGCTCTGGACCTAAACC
CTGAGAGAACTGAGCTCCTGAGCTGCTCCCGTGATGACCTGCTAAAAGTCATCGACCTCCGGACAAATGC
AGTCAAACAGACATTCAGTGCGCCTGGATTCAAATGCGGCTCTGACTGGACCCGGGTTGTCTTCAGCCCT
GATGGCAGTTACGTGGCAGCAGGCTCAGCCGAGGGTTCTCTTTATGTCTGGAGTGTGCTGACAGGGAAAG
TGGAGAAGGTTCTTTCAAAACAGCACAGCTCTTCTATCAATGCGGTGGCGTGGGCCCCCTCGGGCTTACA
TGTTGTCAGTGTGGACAAAGGAAGCAGAGCTGTGCTGTGGGCACAGCCT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG209513 representing BC049122
Red=Cloning site Green=Tags(s)

MSSGLRAADFPRWKRHIAEELRRRDRLQRQAFEEIILQYTKLLEKSDLHSVLTQKLQAEKHDMPNRHEIS
PGHDGAWNDSQLQEMAQLRIKHQEELTELHKKRGELAQLVIDLNNQMQQKDKEIQMNEAKISEYLQTISD
LETNCLDLRTKLQDLEVANQTLKDEYDALQITFTALEEKLRKTTEENQELVTRWMAEKAQEANRLNAENE
KDSRRRQARLQKELAEAAKEPLPVEQDDDIEVIVDETSDHTEETSPVRAVSRAATKRLSQPAGGLLDSIT
NIFGLSESPLLGHHSSDAARRRSVSSIPVPQDIMDTHPASGKDVRVPTTASYVFDAHDGEVNAVQFSPGS
RLLATGGMDRRVKLWEAFGDKCEFKGSLSGSNAGITSIEFDSAGAYLLAASNDFASRIWTVDDYRLRHTL
TGHSGKVLSAKFLLDNARIVSGSHDRTLKLWDLRSKVCIKTVFAGSSCNDIVCTEQCVMSGHFDKKIRFW
DIRSESVVREMELLGKITALDLNPERTELLSCSRDDLLKVIDLRTNAVKQTFSAPGFKCGSDWTRVVFSP
DGSYVAAGSAEGSLYVWSVLTGKVEKVLSKQHSSSINAVAWAPSGLHVVSVDKGSRAVLWAQP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC049122
ORF Size 1869 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC049122, AAH49122
RefSeq Size 3132 bp
RefSeq ORF 1871 bp
Locus ID 77040
Cytogenetics 1 D
Summary Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane (PubMed:18849966, PubMed:12665549, PubMed:24954904, PubMed:24553140, PubMed:23392225). Regulates mitochondrial antiviral signaling (MAVS)-dependent type I interferon (IFN-I) production (By similarity). Negatively regulates NOD1- and NOD2-driven inflammatory cytokine response (PubMed:24238340). Instead, promotes with NOD2 an autophagy-dependent antibacterial pathway. Plays a role in regulating morphology and function of Paneth cell.UniProtKB/Swiss-Prot Function
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"BC049122" in other vectors (4)
SKU Description Size Price
MC218115 Atg16l1 (untagged) - Mouse autophagy-related 16-like 1 (yeast) (cDNA clone MGC:61241 IMAGE:6813377), (10ug) 10 ug
$639.00
MR209513 Atg16l1 (Myc-DDK-tagged) - Mouse autophagy-related 16-like 1 (yeast) (cDNA clone MGC:61241 IMAGE:6813377) 10 ug
$289.00 $580.00
MR209513L3 Lenti ORF clone of Atg16l1 (Myc-DDK-tagged) - Mouse autophagy-related 16-like 1 (yeast) (cDNA clone MGC:61241 IMAGE:6813377) 10 ug
$880.00
MR209513L4 Lenti ORF clone of Atg16l1 (mGFP-tagged) - Mouse autophagy-related 16-like 1 (yeast) (cDNA clone MGC:61241 IMAGE:6813377) 10 ug
$880.00

Plasmid Map for MG209513

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG209513
Circular map for MG209513

file-alt Citations

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