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Pias2 (BC005596) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG209412
Pias2 (tGFP-tagged) - Mouse protein inhibitor of activated STAT 2 (cDNA clone MGC:11445 IMAGE:3969546)
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  $771.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pias2
Synonyms Dib, ARIP3, PIASxbeta, SIZ2, PIASxb, PIASxalpha
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG209412 representing BC005596
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTTTCTAGTTTTAGGGTTTCTGAGTTACAAGTGTTACTGGGCTTTGCTGGACGGAATAAAAGTGGGC
GCAAGCATGACCTCCTGATGAGGGCGTTGCATTTATTGAAGAGTGGCTGCAGCCCTGCGGTTCAGATTAA
AATTCGAGAATTATATAGACGCCGATACCCACGGACACTTGAAGGACTTTGTGATCTTTCCACAATCAAA
TCATCGGTTTTCAGCTTGGATGGTAGCTCATCACCCGTAGAACCTGACTTGCCTGTGGCTGGGATCCACT
CGTTGCCTTCGACTTCAATTACACCTCATTCACCGTCATCTCCTGTTGGTTCTGTACTGCTGCAAGACAC
TAAGCCCACCTTTGAGATGCAGCAGCCGTCTCCGCCCATTCCTCCTGTCCATCCTGATGTGCAGTTAAAA
AACCTGCCTTTCTATGATGTCCTTGATGTTCTCATCAAGCCCACGAGTTTAGTTCAAAGCAGTATTCAGC
GGTTTCAAGAGAAGTTTTTTATTTTTGCTTTGACACCTCAGCAAGTTAGAGAAATATGTATTTCAAGGGA
CTTTTTGCCGGGTGGCAGGAGAGACTACACAGTCCAAGTCCAGCTGCGACTTTGCTTGGCAGAGACCAGT
TGCCCTCAAGAAGATAACTATCCCAATAGTTTGTGTATAAAAGTCAATGGGAAACTCTTTCCTTTGCCTG
GCTATGCGCCACCACCTAAAAATGGGATTGAACAGAAGCGCCCTGGACGCCCCCTGAACATTACGTCTTT
AGTTAGGTTGTCTTCAGCTGTGCCAAATCAGATTTCTATTTCTTGGGCATCTGAAATTGGAAAGAATTAC
TCCATGTCTGTGTATCTTGTACGACAGCTTACATCAGCCATGTTATTACAGAGATTAAAAATGAAAGGTA
TTAGAAATCCTGATCATTCCAGAGCACTAATTAAAGAAAAACTTACTGCAGACCCTGATAGTGAAATTGC
TACAACTAGTCTTCGAGTGTCCTTGATGTGCCCTTTAGGAAAAATGAGGCTGACAATCCCGTGCCGTGCA
GTGACTTGTACACATCTGCAGTGCTTTGATGCTGCCCTCTATCTTCAGATGAATGAGAAAAAGCCCACCT
GGATCTGTCCTGTTTGTGACAAAAAGGCTGCCTATGAGAGTCTGATACTAGATGGGCTTTTTATGGAAAT
TCTCAATGACTGTTCTGATGTGGATGAGATCAAATTCCAGGAAGATGGTTCTTGGTGTCCCATGAGACCT
AAAAAAGAAGCTATGAAAGTAACCAGCCAGCCCTGTACAAAAGTAGAAAGTTCAAGTGTCTTTAGTAAAC
CTTGTTCAGTGACTGTAGCCAGTGATGCAAGCAAGAAGAAGATAGATGTTATTGATCTAACAATAGAGAG
CTCTTCTGATGAAGAGGAAGACCCTCCCGCCAAAAGGAAATGCATCTTTATGTCAGAAACACAAAGCAGT
CCAACCAAAGGGGTTCTCATGTATCAGCCATCTTCTGTAAGGGTGCCCAGTGTGACTTCAGTTGATCCTG
CTGCTATTCCACCTTCATTAACAGACTACTCAGTACCATTCCACCACACGCCAGTGTCAAGCATGTCATC
AGATTTGCCAGGTTTGGATTTTCTTTCCCTTATTCCAGTTGATCCCCAGTACTGTCCTCCTATGTTTTTG
GATAGTCTCACCTCACCCTTAACAGCAAGCAGTACGTCTGTCACCACCACCAGCCCCCATGAAAGCAGTA
CTCACGTTAGTTCATCCAGCAGCAGGAGTGAGACAGGGGTCATAACCAGCAGTGGGAGGAACATTCCTGA
CATCATCTCTTTGGAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG209412 representing BC005596
Red=Cloning site Green=Tags(s)

MVSSFRVSELQVLLGFAGRNKSGRKHDLLMRALHLLKSGCSPAVQIKIRELYRRRYPRTLEGLCDLSTIK
SSVFSLDGSSSPVEPDLPVAGIHSLPSTSITPHSPSSPVGSVLLQDTKPTFEMQQPSPPIPPVHPDVQLK
NLPFYDVLDVLIKPTSLVQSSIQRFQEKFFIFALTPQQVREICISRDFLPGGRRDYTVQVQLRLCLAETS
CPQEDNYPNSLCIKVNGKLFPLPGYAPPPKNGIEQKRPGRPLNITSLVRLSSAVPNQISISWASEIGKNY
SMSVYLVRQLTSAMLLQRLKMKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLMCPLGKMRLTIPCRA
VTCTHLQCFDAALYLQMNEKKPTWICPVCDKKAAYESLILDGLFMEILNDCSDVDEIKFQEDGSWCPMRP
KKEAMKVTSQPCTKVESSSVFSKPCSVTVASDASKKKIDVIDLTIESSSDEEEDPPAKRKCIFMSETQSS
PTKGVLMYQPSSVRVPSVTSVDPAAIPPSLTDYSVPFHHTPVSSMSSDLPGLDFLSLIPVDPQYCPPMFL
DSLTSPLTASSTSVTTTSPHESSTHVSSSSSRSETGVITSSGRNIPDIISLD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC005596
ORF Size 1836 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC005596, AAH05596
RefSeq Size 2269 bp
RefSeq ORF 1838 bp
Locus ID 17344
Cytogenetics 18 E3
Summary Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIASx-beta, but not isoform PIASx-alpha, promotes MDM2 sumoylation. Isoform PIASx-alpha promotes PARK7 sumoylation. Isoform PIASx-beta promotes NCOA2 sumoylation more efficiently than isoform PIASx-alpha (By similarity). Sumoylates PML at'Lys-65' and 'Lys-160' (By similarity).UniProtKB/Swiss-Prot Function
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Documents
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"BC005596" in other vectors (1)
SKU Description Size Price
MC200758 Pias2 (untagged) - Mouse protein inhibitor of activated STAT 2 (cDNA clone MGC:11445 IMAGE:3969546), (10ug) 10 ug
$571.00

Plasmid Map for MG209412

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file-download TXT (FASTA format)

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Cloning scheme for MG209412
Circular map for MG209412

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