Crot (NM_023733) Mouse Tagged ORF Clone

SKU
MG209410
Crot (tGFP-tagged) - Mouse carnitine O-octanoyltransferase (Crot)
  • TrueORF®
    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

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$770.00
3 Weeks*
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Crot
Synonyms 1200003H03Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG209410 representing NM_023733
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAAATCAATTGACTAAATCAGTTGAAGAACGGACATTTCAGTACCAGGACTCTCTTCCGTCATTGC
CTGTTCCTGCACTTGAAGAATCATTAAAAAAATACCTTGAATCAGTGAAACCATTCGCAAATGAAGACGA
GTATAAGAAAACTGAAGAAATAGTTCAAAAGTTTCAAGAAGGAGCTGGCAAAAGATTGCATCAGAAGTTA
CTTGAAAGGGCTAGAGGAAAACGAAACTGGCTGGAAGAATGGTGGCTCAATGTTGCCTACCTGGACGTAC
GTATTCCATCACAACTGAATGTGAACTTTGTGGGTCCGTGTCCTCATTTTGAGCACTACTGGCCTGCAAG
GGAAGGCACTCAGTTAGAAAGAGGAAGCATGATGCTGTGGCACAACTTGAACTACTGGCAGCTGCTAAGA
AGAGAAAAATTGCCTGTACATAAATCTGGAAATACTCCTCTAGACATGAACCAATTCCGAATGTTGTTTT
CTACCTGCAAGGTTCCAGGAATTACTAGAGATTCGATTATGAATTATTTTAAGACTGAGAGTGAAGGGCA
TTGTCCAACCCACATTGCCGTGCTGTGTCGAGGCAGAGCGTTTGTCTTTGATGTACTACATGAGGGATGT
TTGATCACCCCACCAGAACTTCTCAGACAATTGACATATATCCACAAGAAGTGCTCGAACGAACCTGTTG
GACCCAGTATAGCGGCATTAACTAGCGAGGAGCGAACTCGGTGGGCAAAGGCAAGAGAATATCTGATTAG
TCTTGATCCAGAGAACTTGACTTTATTAGAAAAAATTCAAACCAGTTTATTTGTGTATTCCATAGAAGAT
AGCAGTCCACATGCAACCCCAGAAGAATATTCTCAGGTCTTTGAAATGCTTCTTGGTGGAGATCCATCAG
TACGCTGGGGTGACAAGTCCTATAATTTGATTTCCTTTGCTAATGGAATATTTGGCTGTTGCTGTGATCA
TGCTCCTTACGATGCAATGGTCATGGTGAACATTGCACATTATGTTGATGAGAGAGTCCTAGAGACAGAA
GGAAGATGGAAGGGTTCAGAAAAAGTCCGGGACATACCATTGCCAGAGGAGCTGGTCTTCACTGTGGATG
AGAAAATACTGAATGATGTATCCCAAGCCAAAGCCCAACATCTCAAAGCTGCGTCAGATCTGCAGATAGC
AGCATCTACCTTCACATCTTTTGGCAAAAAGCTCACCAAGGAGGAAGCCCTTCACCCTGATACCTTTATT
CAGCTCGCTCTTCAGCTTGCCTACTACAGACTTCATGGACGCCCTGGTTGCTGCTATGAGACAGCTATGA
CAAGATACTTTTACCACGGCCGAACAGAGACTGTGCGATCTTGTACAGTGGAGGCCGTCAGGTGGTGCCA
GTCCATGCAGGATCCCTCTGCTAGTCTCCTTGAACGTCAGCAAAAGATGCTAGAAGCTTTTGCCAAGCAT
AATAAGATGATGAAAGATTGTTCACATGGAAAAGGATTTGACCGTCACCTTTTAGGTCTTTTGCTCATAG
CAAAAGAGGAAGGTCTCCCTGTTCCAGAACTATTTGAGGACCCACTTTTCTCCAGAAGTGGAGGAGGTGG
AAATTTTGTGCTCTCAACAAGTTTGGTTGGTTACTTACGAGTTCAGGGTGTTGTGGTTCCCATGGTACAC
AATGGATATGGATTTTTCTACCACATCAGAGATGACAGGTTTGTGGTGGCATGTTCATCCTGGAGGTCAT
GTCCGGAGACTGATGCTGAAAAGCTTGTTCAGATGATTTTTCATGCTTTCCACGATATGATACAGCTGAT
GAACACCGCTCATCTT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG209410 representing NM_023733
Red=Cloning site Green=Tags(s)

MENQLTKSVEERTFQYQDSLPSLPVPALEESLKKYLESVKPFANEDEYKKTEEIVQKFQEGAGKRLHQKL
LERARGKRNWLEEWWLNVAYLDVRIPSQLNVNFVGPCPHFEHYWPAREGTQLERGSMMLWHNLNYWQLLR
REKLPVHKSGNTPLDMNQFRMLFSTCKVPGITRDSIMNYFKTESEGHCPTHIAVLCRGRAFVFDVLHEGC
LITPPELLRQLTYIHKKCSNEPVGPSIAALTSEERTRWAKAREYLISLDPENLTLLEKIQTSLFVYSIED
SSPHATPEEYSQVFEMLLGGDPSVRWGDKSYNLISFANGIFGCCCDHAPYDAMVMVNIAHYVDERVLETE
GRWKGSEKVRDIPLPEELVFTVDEKILNDVSQAKAQHLKAASDLQIAASTFTSFGKKLTKEEALHPDTFI
QLALQLAYYRLHGRPGCCYETAMTRYFYHGRTETVRSCTVEAVRWCQSMQDPSASLLERQQKMLEAFAKH
NKMMKDCSHGKGFDRHLLGLLLIAKEEGLPVPELFEDPLFSRSGGGGNFVLSTSLVGYLRVQGVVVPMVH
NGYGFFYHIRDDRFVVACSSWRSCPETDAEKLVQMIFHAFHDMIQLMNTAHL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_023733
ORF Size 1836 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_023733.3, NP_076222.1
RefSeq Size 2803 bp
RefSeq ORF 1839 bp
Locus ID 74114
UniProt ID Q9DC50
Cytogenetics 5 A1
Summary Beta-oxidation of fatty acids. The highest activity concerns the C6 to C10 chain length substrate.[UniProtKB/Swiss-Prot Function]
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Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.