OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Mettl25 (NM_207522) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MG209277
Mettl25 (tGFP-tagged) - Mouse cDNA sequence BC067068 (BC067068)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $757.00
3 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (4)

TA150041 Mouse monoclonal turboGFP antibody, clone OTI2H8 100 ul
$447.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100010 pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
10 ug
$780.00

Other products for "Mettl25 or NM_207522"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mettl25
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG209277 representing NM_207522
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGGCCTGCCCTCTGCCCAGGACCCCGGACCTGCCCACGCTGCACGACAAGTTACAGGGCTTGC
TGAGCTTCCTCAGGGGCGCCCTGGCCATTTCCAGCGCGCACACGGTGGACTTCTACACCAAGTCGGTCTG
GCAGGAGCTGGTCGACCTGCCCCCTGAGAGCGTGCTGGCGGCGCTGAGGGAGTCGGCGGTGGAGGCCGAG
CCGCGCGAGGCAGAGACGGGATCAGGTTTCACTGAGCTCCCCAAAATATTTTGTGAAACTTCTCAGAAGC
TGTTGAGTGTGGAAGCTTTTGCCCGAACTGCAAAGCACTATTCTGTGCAAAACTTGGGACTGTGCACTCC
TTCTGAGCAGTTGCTCACAGCCCTCCAAGGAAATAAAAGGCAGAGAGTTGATGAAAATGTGAAGGCAATT
GAGTTTATGAATACAAAAAAATCTCATGAGGTTCAGGCAATGTCAGAGCTAATCTGCAGTATTGCTGATT
ACTGTGGCTTAAAGCAGATAATTGACGTGGGTTCTGGTAAAGGCTATCTAAGCTCCTTTTTGTCCTTAAA
ATATGGTTTGAACGTTTATGGCATCGATTCCTCAAACACTAACACTCACGGCGCGAAGGAGAGAAACAGG
AAGTTGAAGAAGCATTGGAGCCTGTATCACCCTCACTCAAGAGCAGACGCCAACGGCTGGGCATCAGAAA
GGCCCAGAGAGTTGAAAGTGCCAAAGGGAGTGGAGTGTAAGGGGGACGCTGAGAGCGTGCAGAGGAGCAG
GCTTGGGAATCCAGACCTGTCTGCCACCGATGGTTTGCCTGATTTCTCAGGCTCTGCAATTTCAGTCATC
AGAAAACAGCAAAAAAACGTGCTTGCTCAGCCAGCTGAGGAAGAAAATTTGTATTTTGAAGATGCCTTTT
CTCTCATAGACTTTTTACCTGTTGATGCCATCGAACCTACTTCATCACAGGTGCAGAACACAGAAAAGTC
TGGACTGAGAAAAGAGAGAAGAAATACGGCATCCAAGGCAAGGGACTCAAGCATATATTCACCACTAACC
TCTTTCATCACTGCTGATTCACAATTGCATGACATTATTGAAGATCTGGAGGACTGTTTAATGGTGGGAC
TGCATACCTGTGGTGACCTTGCACCCAGTACTCTGCGGATATTTACATCCAAGGCTGAAGTCAAGGCAGT
TTGCAGTGTGGGTTGTTGCTACCATCTCCTATCTGAGGAGTTTGAAAACCAGCATAAAGATAGATGTGCT
AACGAAAACTGGGGGTTTCCAATGTGCCACTATCTGAAAGAGGAGAGATGGTGCTGCGGCCGTAATGCAA
GAATGTCAGCATGTTTGGCATTGCAGCGGGTTGCAGTTGGCCAAGGGCTGCCTACTGAATCACTCTTCTA
TCGTGCTGTTCTTCAGAATATTATTAAGGATTATTATGGCATCAGCAAATGTGAACAGCACGTTGGTAAA
ATTTATTCCAAATGTTCCTCTTTTCTGGAGTATGTCAGAATGTCTCTAAAGAAGCTTGGATTAGATGAGT
CCAAGGTCTCTGAAGAAATTATAATGGACTACTATGAGAATTATAAGCCTAGAATGAATGAGCTAGAGGC
ATTTAATATGTTGAAAGTTGTTCTGGCTCCTTGCATAGAAACACTGATTCTTCTTGATCGACTTTGTTAC
CTGAAGGAACAGGACGGTGTAGCCTGGTCTGCCCTCGTGAAGTTGTTTGACCCTGTGCAGTCGCCCAGAT
GCTACGCTGTCATTGCTCTGAAGAAGCAGTGTGATCTTGGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG209277 representing NM_207522
Red=Cloning site Green=Tags(s)

MAAACPLPRTPDLPTLHDKLQGLLSFLRGALAISSAHTVDFYTKSVWQELVDLPPESVLAALRESAVEAE
PREAETGSGFTELPKIFCETSQKLLSVEAFARTAKHYSVQNLGLCTPSEQLLTALQGNKRQRVDENVKAI
EFMNTKKSHEVQAMSELICSIADYCGLKQIIDVGSGKGYLSSFLSLKYGLNVYGIDSSNTNTHGAKERNR
KLKKHWSLYHPHSRADANGWASERPRELKVPKGVECKGDAESVQRSRLGNPDLSATDGLPDFSGSAISVI
RKQQKNVLAQPAEEENLYFEDAFSLIDFLPVDAIEPTSSQVQNTEKSGLRKERRNTASKARDSSIYSPLT
SFITADSQLHDIIEDLEDCLMVGLHTCGDLAPSTLRIFTSKAEVKAVCSVGCCYHLLSEEFENQHKDRCA
NENWGFPMCHYLKEERWCCGRNARMSACLALQRVAVGQGLPTESLFYRAVLQNIIKDYYGISKCEQHVGK
IYSKCSSFLEYVRMSLKKLGLDESKVSEEIIMDYYENYKPRMNELEAFNMLKVVLAPCIETLILLDRLCY
LKEQDGVAWSALVKLFDPVQSPRCYAVIALKKQCDLG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_207522
ORF Size 1791 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_207522.1, NP_997405.1
RefSeq Size 1969 bp
RefSeq ORF 1803 bp
Locus ID 216292
UniProt ID Q6NXH8
Cytogenetics 10 D1
Summary Putative methyltransferase.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_207522" in other vectors (4)
SKU Description Size Price
MC205252 Mettl25 (untagged) - Mouse cDNA sequence BC067068 (BC067068), (10ug) 10 ug
$557.00
MR209277 Mettl25 (Myc-DDK-tagged) - Mouse cDNA sequence BC067068 (BC067068) 10 ug
$289.00 $557.00
MR209277L3 Lenti ORF clone of Mettl25 (Myc-DDK-tagged) - Mouse cDNA sequence BC067068 (BC067068) 10 ug
$857.00
MR209277L4 Lenti ORF clone of Mettl25 (mGFP-tagged) - Mouse cDNA sequence BC067068 (BC067068) 10 ug
$857.00

Plasmid Map for MG209277

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG209277
Circular map for MG209277

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products