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Atad3a (NM_179203) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG209210
Atad3a (tGFP-tagged) - Mouse ATPase family, AAA domain containing 3A (Atad3a)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $751.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Atad3a or NM_179203"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Atad3a
Synonyms 2400004H09Rik; Atad3; mKIAA1273; Tob3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG209210 representing NM_179203
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGTGGCTCTTCGGCATCAAGGGCCCCAAGGGCGAAGGCACAGGGCCTCCGCTGCCCTTGCCGCCCG
CTCAACCCGGGGCGGAGGGCGGCGGTGACCGCGGGGCGGGAGACCGGCCATCGCCCAAGGACAAATGGAG
CAACTTCGACCCGACGGGCCTGGAACGTGCGGCCAAAGCGGCTCGCGAGTTGGAGCACTCGCGCCATGCC
AAGGAGGCACTGAGTCTCGCACAGATGCAGGAGCAGACGCTGCAGCTGGAACAGCAGTCCAAGCTCAAGG
AGTACGAAGCTGCCGTAGAGCAGCTGAAGAGCGAACAGATCCGTGTGCAAGCCGAGGAAAGAAGGAAAAC
CCTGACTGAAGAGACACGACAGCACCAGGCTAGGGCCCAGTACCAGGATAAGCTCGCTCGACAGCGCTAT
GAGGACCAGCTGAAACAACAGCAACTTCTGAATGAAGAGAATTTAAGGAAACAAGAGGAGTCTGTGCAGA
AGCAGGAGGCCATACGGCGAGCCACTGTGGAGCGCGAGATGGAGCTGAGGCATAAAAACGAGATGTTGCG
GGTGGAAGCTGAAGCCCGAGCACGGGCCAAGGCTGATCGAGAGAATGCAGATATCATCCGGGAACAGATT
CGACTCAAGGCTGCTGAGCACCGCCAGACCATCTTGGAGTCTATCAGGACAGCTGGCACCTTGCTTGGTG
AAGGATTCCGTGCATTTGTGACAGACTGGGACAAAGTGACAGCTACGGTGGCTGGGTTGACACTATTAGC
TGTTGGAGTCTATTCTGCAAAGAATGCTACTTCTGTTGCTGGTCGGTATATTGAGGCCCGATTGGGAAAG
CCGTCCTTGGTGAGAGAGACCTCCCGAATCTCAGTGCTAGAGGCATTGAGGCATCCCATCCAGGTCAGCA
GGCGACTGGTCAGCAGACCCCAGGATGCATTGGAGGGCGTCATCCTCAGTCCTAGCCTGGAGGCACGGGT
CCGAGATATTGCCATCGCAACAAGAAATACCAAGAAGAACAAAAGCCTGTATAGGAACGTTCTGATGTAT
GGGCCCCCGGGGACTGGCAAGACACTATTTGCCAAGAAACTTGCACTGCATTCAGGCATGGACTACGCCA
TCATGACAGGCGGGGACGTGGCCCCAATGGGGCGGGAGGGTGTGACTGCCATGCACAAGGTCTTCGACTG
GGCAAGCACCAGCCGACGAGGCCTCCTGCTCTTTGTGGATGAAGCAGACGCCTTCCTCAGGAAACGAGCG
ACTGAAAAGATAAGTGAAGACCTCAGGGCTACTCTGAATGCCTTCCTACACAGGACAGGACAGCACAGTA
GTAAGTTCATGCTGGTCCTGGCCAGTAACCAGCCTGAGCAGTTTGATTGGGCTATCAATGACCGCATTGA
CGAGATGGTCTGCTTTGCCCTGCCACAGCGGGAGGAGCGAGAGCGCCTGGTGAGAATGTATTTTGACAAG
TATGTCCTTAAGCCGGCCACAGAAGGAAAGCAGCGCTTGAAGGTGGCCCAGTTTGACTATGGAAAGAAAT
GCTCAGAGGTTGCCCAGCTAACGGAGGGGATGTCAGGCCGGGAGATTGCTCAGCTTGCTGTGGCGTGGCA
GGCCATGGCATATTCATCTGAGGATGGAGTCCTCACGGAAGCTATGATGGATGCCCGTGTGCAGGATGCT
GTGCAGCAGCACCAGCAGAAGATGCAGTGGCTTAAAGTAGAGAGACCCGATTCCCAGACCAACAAGCCGC
CACATCCTTCACTCCTCAGCTGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG209210 representing NM_179203
Red=Cloning site Green=Tags(s)

MSWLFGIKGPKGEGTGPPLPLPPAQPGAEGGGDRGAGDRPSPKDKWSNFDPTGLERAAKAARELEHSRHA
KEALSLAQMQEQTLQLEQQSKLKEYEAAVEQLKSEQIRVQAEERRKTLTEETRQHQARAQYQDKLARQRY
EDQLKQQQLLNEENLRKQEESVQKQEAIRRATVEREMELRHKNEMLRVEAEARARAKADRENADIIREQI
RLKAAEHRQTILESIRTAGTLLGEGFRAFVTDWDKVTATVAGLTLLAVGVYSAKNATSVAGRYIEARLGK
PSLVRETSRISVLEALRHPIQVSRRLVSRPQDALEGVILSPSLEARVRDIAIATRNTKKNKSLYRNVLMY
GPPGTGKTLFAKKLALHSGMDYAIMTGGDVAPMGREGVTAMHKVFDWASTSRRGLLLFVDEADAFLRKRA
TEKISEDLRATLNAFLHRTGQHSSKFMLVLASNQPEQFDWAINDRIDEMVCFALPQREERERLVRMYFDK
YVLKPATEGKQRLKVAQFDYGKKCSEVAQLTEGMSGREIAQLAVAWQAMAYSSEDGVLTEAMMDARVQDA
VQQHQQKMQWLKVERPDSQTNKPPHPSLLSC

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_179203
ORF Size 1773 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_179203.3
RefSeq Size 2414 bp
RefSeq ORF 1776 bp
Locus ID 108888
UniProt ID Q925I1
Cytogenetics 4 E2
Summary Essential for mitochondrial network organization, mitochondrial metabolism and cell growth at organism and cellular level. May play an important role in mitochondrial protein synthesis. May also participate in mitochondrial DNA replication. May bind to mitochondrial DNA D-loops and contribute to nucleoid stability. Required for enhanced channeling of cholesterol for hormone-dependent steroidogenesis (By similarity).UniProtKB/Swiss-Prot Function
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Resources
"NM_179203" in other vectors (4)
SKU Description Size Price
MC205186 Atad3a (untagged) - Mouse ATPase family, AAA domain containing 3A (Atad3a), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$551.00
MR209210 Atad3a (Myc-DDK-tagged) - Mouse ATPase family, AAA domain containing 3A (Atad3a), nuclear gene encoding mitochondrial protein 10 ug
$289.00 $551.00
MR209210L3 Lenti ORF clone of Atad3a (Myc-DDK-tagged) - Mouse ATPase family, AAA domain containing 3A (Atad3a), nuclear gene encoding mitochondrial protein 10 ug
$851.00
MR209210L4 Lenti ORF clone of Atad3a (mGFP-tagged) - Mouse ATPase family, AAA domain containing 3A (Atad3a), nuclear gene encoding mitochondrial protein 10 ug
$851.00

Plasmid Map for MG209210

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG209210
Circular map for MG209210

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