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Galnt4 (NM_015737) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG209072
Galnt4 (tGFP-tagged) - Mouse UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4 (Galnt4)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $739.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Galnt4
Synonyms AV011803
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG209072 representing NM_015737
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGTGAGGTGGACCTGGGCAGGCAAGAGCTGCCTGCTGCTGGCCCTTTTAACACTGGCTTATATTC
TGGTGGAGTTCTCAGTCTCCACTTTGTATGCCTCTCCAGGAGCCGGCGGTGCCAGGGAGCTGGGGCCAAG
GCGGCTCCCGGACCTTGATACAAGGGAAGAGGATTTGTCTCAGCCTCTTTATATAAAGCCCCCTGCAGAT
TCCCATGCTCTTGGGGAATGGGGGAGAGCAAGCAAACTCCAGCTCAACGAGGGGGAACTGAAGCAGCAAG
AAGAACTTATTGAGAGATATGCCATCAACATTTACCTCAGTGACAGGATCTCCCTGCACCGCCACATAGA
GGATAAAAGAATGTATGAGTGTAAAGCCAAGAAGTTCCACTACAGGTCGCTCCCCACCACCTCCGTTATC
ATCGCCTTCTATAACGAAGCCTGGTCCACTCTGCTTCGGACCATTCACAGTGTTTTAGAAACTTCTCCAG
CTGTGCTTTTAAAGGAGATCATCTTGGTGGATGACTTGAGTGATAGAATTTATTTGAAGGCGCAACTTGA
AACTTACATCAGCAACCTGGAGAGAGTTCGCTTGATTAGAACCAATAAGAGAGAGGGGCTGGTCCGGGCC
CGTCTAATTGGGGCCACTTTTGCCACCGGGGATGTCCTCACTTTCCTGGATTGTCACTGTGAGTGTAACA
CTGGTTGGTTGGAACCACTTTTGGAGAGGATCAGTAGAGACGAAACCGCAATCGTATGTCCTGTCATCGA
CACTATTGACTGGAACACTTTCGAATTCTACATGCAGACCGGGGAGCCCATGATTGGTGGGTTTGACTGG
CGTTTGACCTTCCAGTGGCATTCTGTCCCCAAACATGAAAGGGACAGGCGAACGTCAAGAATTGACCCCA
TCAGGTCCCCCACCATGGCAGGAGGACTGTTTGCTGTCAGCAAGAAGTATTTTCAGTACCTGGGCACCTA
TGACACTGGCATGGAAGTCTGGGGAGGCGAAAACCTTGAGTTGTCTTTCAGGGTTTGGCAGTGTGGCGGC
AAGCTGGAGATCCACCCATGCTCGCACGTGGGCCACGTGTTTCCCAAGCGCGCGCCCTATGCTCGCCCCA
ACTTCCTACAGAATACCGCGCGGGCGGCCGAAGTGTGGATGGATGAATACAAAGAGCATTTCTATAACCG
AAACCCCCCAGCTCGGAAAGAGGCCTATGGCGATCTTTCTGAAAGGAAGTTACTAAGGGAACGGCTGAAA
TGTAAGAGCTTCGACTGGTATCTGAAAAATGTGTTCTCTAACTTACACGTCCCCGAGGATAGACCTGGCT
GGCACGGAGCCATCCGCAGCATGGGCATCTCTTCCGAGTGCTTAGATTATAACGCTCCCGACAACAACCC
TACGGGGGCTAATCTTTCGTTGTTTGGATGCCACGGTCAAGGAGGCAATCAGTTCTTTGAATATACTTCA
AACAAAGAAATCAGATTCAATTCCGTGACTGAATTATGTGCAGAGGTCCCCCAACAAAAGGATTATGTAG
GGATGCAAAACTGCCCCAAAGACGGGCTCCCAGTTCCAGTGAACATCATTTGGCATTTTAAAGAAGACGG
AACGATTTTTCACCCTCACACAAGACTGTGTCTTAGTGCTTACCGGACAGCCGAGGGCCGACCTAGTGTC
CATATGAAAACGTGTGATGCCCTTGATAAAAACCAGCTCTGGAGGTTTGAGAAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG209072 representing NM_015737
Red=Cloning site Green=Tags(s)

MAVRWTWAGKSCLLLALLTLAYILVEFSVSTLYASPGAGGARELGPRRLPDLDTREEDLSQPLYIKPPAD
SHALGEWGRASKLQLNEGELKQQEELIERYAINIYLSDRISLHRHIEDKRMYECKAKKFHYRSLPTTSVI
IAFYNEAWSTLLRTIHSVLETSPAVLLKEIILVDDLSDRIYLKAQLETYISNLERVRLIRTNKREGLVRA
RLIGATFATGDVLTFLDCHCECNTGWLEPLLERISRDETAIVCPVIDTIDWNTFEFYMQTGEPMIGGFDW
RLTFQWHSVPKHERDRRTSRIDPIRSPTMAGGLFAVSKKYFQYLGTYDTGMEVWGGENLELSFRVWQCGG
KLEIHPCSHVGHVFPKRAPYARPNFLQNTARAAEVWMDEYKEHFYNRNPPARKEAYGDLSERKLLRERLK
CKSFDWYLKNVFSNLHVPEDRPGWHGAIRSMGISSECLDYNAPDNNPTGANLSLFGCHGQGGNQFFEYTS
NKEIRFNSVTELCAEVPQQKDYVGMQNCPKDGLPVPVNIIWHFKEDGTIFHPHTRLCLSAYRTAEGRPSV
HMKTCDALDKNQLWRFEK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015737
ORF Size 1734 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015737.4
RefSeq Size 5111 bp
RefSeq ORF 1737 bp
Locus ID 14426
UniProt ID O08832
Cytogenetics 10 C3
Summary Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has a highest activity toward EA2 peptide substrate and a much lower activity with EPO-T, Muc2, Muc1a, Muc1b.UniProtKB/Swiss-Prot Function
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Documents
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"NM_015737" in other vectors (4)
SKU Description Size Price
MC202379 Galnt4 (untagged) - Mouse UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4 (Galnt4), (10ug) 10 ug
$539.00
MR209072 Galnt4 (Myc-DDK-tagged) - Mouse UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4 (Galnt4) 10 ug
$289.00 $539.00
MR209072L3 Lenti ORF clone of Galnt4 (Myc-DDK-tagged) - Mouse UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4 (Galnt4) 10 ug
$839.00
MR209072L4 Lenti ORF clone of Galnt4 (mGFP-tagged) - Mouse UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4 (Galnt4) 10 ug
$839.00

Plasmid Map for MG209072

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG209072
Circular map for MG209072

file-alt Citations

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