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Sh3bp2 (NM_011893) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG208857
Sh3bp2 (tGFP-tagged) - Mouse SH3-domain binding protein 2 (Sh3bp2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $721.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sh3bp2
Synonyms 3BP2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG208857 representing NM_011893
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCTGAGGAGATGCACTGGCCTGTCCCCATGAAGGCCATTGGTGCCCAGAATTTGCTGACCATGC
CTGGTGGTGTGGCCAAGGCTGGTTACCTGCATAAGAAGGGTGGCACCCAACTGCAGCTGCTCAAATGGCC
CCTCCGCTTTGTGATCATCCACAAGCGCTGCATCTACTACTTCAAGAGCAGCACATCTGCCTCCCCACAG
GGTGCCTTCTCACTGAGTGGCTACAACAGAGTGATGCGAGCAGCTGAGGAGACCACGTCCAACAATGTCT
TCCCATTCAAGATCATTCACATCAGCAAGAAGCACCGAACATGGTTCTTCTCAGCGTCCTCTGAGGATGA
GCGCAAGAGCTGGATGGCCTTGCTACGCAGGGAAATTGGCCACTTCCATGAGAAGAAGGAACTACCTCTA
GACACCAGTGACTCTAGCTCAGACACGGACAGCTTCTATGGTGCAGTTGAGCGACCTATAGATATCAGCC
TGTCTTCATATCCCATGGACAATGAAGATTACGAACATGAAGATGAGGATGACTCGTACCTGGAGCCTGA
TTCCCCGGGGCCCATGAAGCTTGAAGATGCCCTGACCTATCCACCAGCCTACCCACCACCCCCTGTGCCT
GTACCTCGGAAGCCAGCCTTCTCTGACTTGCCCCGTGCCCACTCGTTCACCTCTAAAAGTCCAAGTCCCT
TACTGCCACCCCCACCTCCTAAGCGAGGCCTTCCAGACACTGGCCCTGCCCCTGAGGATGCTAAGGACGC
ATTGGGCCTGAGGCGAGTAGAGCCTGGCCTCAGGGTGCCTGCCACCCCTCGGAGGATGAGTGACCCCCCA
ATGAGCAATGTACCCACCGTGCCCAACCTCCGGAAACATCCTTGCTTCCGGGACAGTGTGAATCCCGGCC
TAGAACCCTGGACCCCTGGCCATGGGACTAGCTCTGTCTCCAGCTCTACCACCATGGCCGTTGCCACCTC
TAGGAACTGTGACAAACTCAAGTCATTCCACCTGTCTTCCCGAGGGCCACCCACATCTGAGCCCCCACCC
GTGCCTGCTAATAAGCCCAAGTTCCTGAAGATAGCTGAAGAACCTTCCCTGAGGGAGGCAGCCAAGTTTG
CACCTGTGCCCCCTGTGGCCCCCAGGCCTCCTGTGCAGAAGATGCCCATGCCTGAGGCTACTGTTCGGCC
TGCAGTGCTGCCTAGACCAGAGAACACACCTCTTCCTCACCTCCAGCGATCCCCCCCTGATGGACAGAGT
TTCAGGGGCTTCTCCTTTGAAAAGGCCCGCCAGCCCTCACAGGCTGACACTGGTGAGGAGGACTCAGATG
AAGACTATGAGAAGGTGCCGCTGCCTAACTCGGTGTTTGTCAACACGACAGAGTCCTGCGAGGTGGAAAG
GTTGTTCAAAGCTACGGACCCCCGTGGAGAGCCTCAGGATGGGCTGTATTGCATTCGGAACTCCTCTACC
AAGTCAGGGAAGGTTTTGGTCGTGTGGGATGAATCTTCTAACAAAGTGAGGAATTATCGCATCTTTGAAA
AGGATTCTAAGTTCTACCTGGAAGGTGAGGTTCTGTTTGCCAGTGTGGGCAGCATGGTGGAGCACTACCA
CACCCATGTGCTGCCAAGCCATCAGAGCCTGTTGCTGCGGCACCCATACGGCTACGCTGGGCCCAGG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG208857 representing NM_011893
Red=Cloning site Green=Tags(s)

MAAEEMHWPVPMKAIGAQNLLTMPGGVAKAGYLHKKGGTQLQLLKWPLRFVIIHKRCIYYFKSSTSASPQ
GAFSLSGYNRVMRAAEETTSNNVFPFKIIHISKKHRTWFFSASSEDERKSWMALLRREIGHFHEKKELPL
DTSDSSSDTDSFYGAVERPIDISLSSYPMDNEDYEHEDEDDSYLEPDSPGPMKLEDALTYPPAYPPPPVP
VPRKPAFSDLPRAHSFTSKSPSPLLPPPPPKRGLPDTGPAPEDAKDALGLRRVEPGLRVPATPRRMSDPP
MSNVPTVPNLRKHPCFRDSVNPGLEPWTPGHGTSSVSSSTTMAVATSRNCDKLKSFHLSSRGPPTSEPPP
VPANKPKFLKIAEEPSLREAAKFAPVPPVAPRPPVQKMPMPEATVRPAVLPRPENTPLPHLQRSPPDGQS
FRGFSFEKARQPSQADTGEEDSDEDYEKVPLPNSVFVNTTESCEVERLFKATDPRGEPQDGLYCIRNSST
KSGKVLVVWDESSNKVRNYRIFEKDSKFYLEGEVLFASVGSMVEHYHTHVLPSHQSLLLRHPYGYAGPR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011893
ORF Size 1677 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011893.2, NP_036023.2
RefSeq Size 2888 bp
RefSeq ORF 1680 bp
Locus ID 24055
Cytogenetics 5 B2
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Documents
Resources
"NM_011893" in other vectors (4)
SKU Description Size Price
MC200727 Sh3bp2 (untagged) - Mouse SH3-domain binding protein 2 (Sh3bp2), transcript variant 1, (10ug) 10 ug
$521.00
MR208857 Sh3bp2 (Myc-DDK-tagged) - Mouse SH3-domain binding protein 2 (Sh3bp2), transcript variant 1 10 ug
$289.00 $521.00
MR208857L3 Lenti ORF clone of Sh3bp2 (Myc-DDK-tagged) - Mouse SH3-domain binding protein 2 (Sh3bp2), transcript variant 1 10 ug
$821.00
MR208857L4 Lenti ORF clone of Sh3bp2 (mGFP-tagged) - Mouse SH3-domain binding protein 2 (Sh3bp2), transcript variant 1 10 ug
$821.00

Plasmid Map for MG208857

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG208857
Circular map for MG208857

file-alt Citations

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