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Ncaph2 (BC003900) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG208828
Ncaph2 (tGFP-tagged) - Mouse non-SMC condensin II complex, subunit H2 (cDNA clone MGC:6766 IMAGE:3601298)
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  $719.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ncaph2
Synonyms MGC6766
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG208828 representing BC003900
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACTTCATTGAGGCAGCACTGTTGATCCAGGGCTCAGCCTGTGTCTACAGTAAGAAGGTGGAGTACC
TCTACTCGCTGGTCTACCAGGCTCTCGATTTTATTTCTGGCAAGAGGCGGGCCAAACAGCTCTCCTTAGT
TCAGGAAGATGGGAGCAAGAAGACTGTCAACTCAGAGACTCCCTGTGAAACAGAGAATGAGTTCCTGTCA
CTGGACGACTTCCCTGACTCCCGGGCTAATGTGGATCTGAAAAATGATCAGGCATCCAGTGAGCTGCTTA
TCATACCCCTACTGCCCATGGCCCTGGTGGCCCCTGATGAAGTGGAAAAGAACAGCAGCCCCTTGTATAG
CTGTCAGGGTGACATCTTGGCCAGCCGGAAGGATTTCAGGATGAACACGTGTATGCCTAACCCCAGAGGC
TGCTTTATGTTAGATCCAGTGGGAATGTGTCCTGTGGAGCCTGTGGTGCCCGTGGAGCCATACCCCATGT
CAAGGAGCCAGAAAGATCCTGAGGACGCTGAGGAGCAGCCCATGGAAGTGTCTAGGAACGGGAGTCCTGT
TCCTGTACCCGACATCTCCCAAGAGCCAGATGGTCCAGCGCTCAGCGGTGGAGAGGAGGATGCAGAGGAT
GGAGCAGAGCCCCTGGAGGTTGCTCTAGAGCCTGCAGAGCCAAGGACCTCACAGCAGAGTGCCATCTTGC
CAAGGAGATACATGCTGCGGGAACGACAAGGGGCACCGGAGCCTGCCTCCCGGCTACAGGAGACCCCAGA
CCCCTGGCAGAGCCTGGACCCTTTTGACTCCTTGGAATCTAAGGTCTTCCAGAAAGGGAAACCCTATTCT
GTGCCACCCGGTGTGGAGGAGGCTCCAGGACAGAAGCGCAAGAGGAAGGGTGCCACCAAGTTGCAGGACT
TCCACAAGTGGTACCTGGATGCCTATGCTGAACACCCTGACGGCAGGAGGGCTCGGCGGAAGGGCCCAAC
CTTTGCAGACATGGAAGTCCTGTACTGGAAACATGTGAAAGAACAGCTCGAGACCCTTCAGAAGCTGCGG
AGACGTAAGATCAACGAGAGATGGCTACCTGGGGCCAAGCAGGATCTGTGGCCTACAGAGGAGGATCGCT
TGGAAGAGTCCCTCGAGGACCTCGGGGTAGCAGATGACTTTCTAGAGCCCGAGGAGTACGTGGAGGAGCC
TGCGGGGGTGATGCCCGAGGAAGCTGCTGACCTCGATGCAGAGGCCATGCCAGAGTCCCTGAGATACGAG
GAGCTGGTCCGGAGAAATGTGGAACTCTTCATTGCCACCTCCCAGAAGTTTATCCAGGAGACAGAGCTGA
GCCAACGCATCAGGGACTGGGAAGATACCATCCAGCCCCTGCTCCAGGAGCAGGAGCAGCATGTGCCCTT
TGATATCCATATCTACGGGGACCAGTTGGCTTCACGGTTCCCCCAGCTCAATGAATGGTGTCCCTTTTCA
GAGCTTGTAGCAGGGCAGCCTGCTTTTGAGGTGTGCCGCTCCATGCTGGCCTCCTTGCAACTGGCTAATG
ACTACACAGTGGAGATCACTCAGCAGCCAGGACTGGAGGCAGCTGTGGACACAATGTCTCTGAGACTGCT
CACACACCAGCGAGCCCACACCCGCTTCCAGACCTATGCTGCACCATCCATGGCCCAGCCT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG208828 representing BC003900
Red=Cloning site Green=Tags(s)

MNFIEAALLIQGSACVYSKKVEYLYSLVYQALDFISGKRRAKQLSLVQEDGSKKTVNSETPCETENEFLS
LDDFPDSRANVDLKNDQASSELLIIPLLPMALVAPDEVEKNSSPLYSCQGDILASRKDFRMNTCMPNPRG
CFMLDPVGMCPVEPVVPVEPYPMSRSQKDPEDAEEQPMEVSRNGSPVPVPDISQEPDGPALSGGEEDAED
GAEPLEVALEPAEPRTSQQSAILPRRYMLRERQGAPEPASRLQETPDPWQSLDPFDSLESKVFQKGKPYS
VPPGVEEAPGQKRKRKGATKLQDFHKWYLDAYAEHPDGRRARRKGPTFADMEVLYWKHVKEQLETLQKLR
RRKINERWLPGAKQDLWPTEEDRLEESLEDLGVADDFLEPEEYVEEPAGVMPEEAADLDAEAMPESLRYE
ELVRRNVELFIATSQKFIQETELSQRIRDWEDTIQPLLQEQEQHVPFDIHIYGDQLASRFPQLNEWCPFS
ELVAGQPAFEVCRSMLASLQLANDYTVEITQQPGLEAAVDTMSLRLLTHQRAHTRFQTYAAPSMAQP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC003900
ORF Size 1671 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC003900, AAH03900
RefSeq Size 2063 bp
RefSeq ORF 1673 bp
Locus ID 52683
Cytogenetics 15 44.84 cM
Summary This gene encodes a component of the condensin-2 complex. The encoded protein may regulate the structure of mitotic chromosomes. Loss of function of this gene disrupts T-cell development. There are two pseudogenes for this gene on chromosome 17. Alternative splicing results in multiple transcript variants. provided by RefSeq, Oct 2012
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Documents
Resources
"BC003900" in other vectors (1)
SKU Description Size Price
MC200537 Ncaph2 (untagged) - Mouse non-SMC condensin II complex, subunit H2 (cDNA clone MGC:6766 IMAGE:3601298), (10ug) 10 ug
$519.00

Plasmid Map for MG208828

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG208828
Circular map for MG208828

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