Stam (NM_011484) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG208719
Stam (tGFP-tagged) - Mouse signal transducing adaptor molecule (SH3 domain and ITAM motif) 1 (Stam)
  $711.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Stam
Synonyms STAM1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG208719 representing NM_011484
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTCTCTTTGCCACCAACCCCTTCGACCAGGATGTTGAGAAAGCAACTAGTGAGTTGAATACTGCTG
AGGACTGGGGCCTCATCTTGGATATTTGTGATAAGGTTGGCCAATCTCGCACAGGACCTAAAGATTGTCT
TCGTTCCATTATGAGAAGAGTGAACCATAAAGACCCCCATGTTGCTATGCAAGCCTTAACTCTTCTGGGA
GCATGTGTATCAAACTGTGGCAAAATTTTTCACTTAGAAGTATGTTCAAGAGACTTTGCTAGTGAAGTAA
GCAATGTATTAAATAAGGGTCATCCTAAAGTATGTGAAAAATTAAAGGCTCTTATGGTTGAATGGACAGA
TGAATTTAAGAATGATCCACAGCTTAGTCTGATATCGGCAATGATCAAGAACCTTAAGGAACAAGGTGTA
ACATTCCCAGCTATTGGCTCTCAGGCTGCAGAACAAGCAAAAGCAAGCCCAGCTCTCGTAGCCAAGGACC
CTGGGACTGTGGCTACCAAAAAGGAAGAAGAAGATTTAGCCAAAGCTATTGAGTTGTCCCTGAAGGAGCA
AAGGCAGCAGTCAGCCCCCGTGTCCACGCTGTACCCCAGCACATCTAACCTCCTCACTAACCACCAGCAC
GAAGGCAGGAAAGTCCGCGCCGTGTACGATTTTGAAGCCGCTGAGGATAATGAGCTTACCTTTAAAGCTG
GAGAAATCATTACAGTCCTTGATGACAGTGATCCCAACTGGTGGAAAGGTGAAACCCATCAAGGCGTGGG
GCTTTTTCCTTCTAATTTTGTAACTGCAGATCTTACTGCTGAACCAGAAATGATTAAAACAGAGAAGAAG
ACGGTACAGTTCAATGATGATGTTCAGATAGAGACAATAGAACCAGAACCAGAACCAGCCTTTATTGATG
AGGATAAAATGGACCAGTTGTTACAGATGTTGCAAAGTACAGATCCTAGTGATAATCAGCCAGACTTGCC
AGAGCTGCTTCATCTTGAAGCAATGTGTCACCAGATGGGACCTCTCATTGATGAAAAGCTGGAAGATATT
GATAGAAAACATTCAGAACTGTCAGAACTAAACGTGAAGGTGATGGAAGCTCTCTCACTGTATACGAAGT
TAATGAATGAAGACCCAATGTACTCTATGTATGCAAAATTACAGAGTCAGCAGTACTATCTGCAGTCGTC
GGCTGTCTCTGCTTCCCAGGTATATCCAGGGCCTGCGCAGAGCGGTACCTACCTGGTTGCAGGGAGTGCA
CAGATGACCCATCTGCAGAGCTACAGCCTTCCCCCAGAGCAGCTGTCTTCTATCAGCCAAGGAGCAGTTC
CATCCTCTGCAAATCAAGCTCTTCCTAGTCAGCAGACCCAGGCTTCTTACCCGAATGCAATGGTTAGTTC
TGTTCAAGGAAATTCGTATCCTAGCCAGGCTTCAATATATAGTCCTCCTGCTGCTGCTGCTGCTGCCGCT
GCTGCTGCTGTAGTACCTGTTCCTGTACCTGCTGATGTCACCATATACCAGAATGCAGGACCTACTATGT
CCCAGGTGCCAAACTATACTTTAACATCATCAACCCTGCCTCAAACAGGAGGCAGCCAACAGCCACCTCA
GCCACAGCAAGCATATTCTCAGAAGGCTCTGCTA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG208719 representing NM_011484
Red=Cloning site Green=Tags(s)

MPLFATNPFDQDVEKATSELNTAEDWGLILDICDKVGQSRTGPKDCLRSIMRRVNHKDPHVAMQALTLLG
ACVSNCGKIFHLEVCSRDFASEVSNVLNKGHPKVCEKLKALMVEWTDEFKNDPQLSLISAMIKNLKEQGV
TFPAIGSQAAEQAKASPALVAKDPGTVATKKEEEDLAKAIELSLKEQRQQSAPVSTLYPSTSNLLTNHQH
EGRKVRAVYDFEAAEDNELTFKAGEIITVLDDSDPNWWKGETHQGVGLFPSNFVTADLTAEPEMIKTEKK
TVQFNDDVQIETIEPEPEPAFIDEDKMDQLLQMLQSTDPSDNQPDLPELLHLEAMCHQMGPLIDEKLEDI
DRKHSELSELNVKVMEALSLYTKLMNEDPMYSMYAKLQSQQYYLQSSAVSASQVYPGPAQSGTYLVAGSA
QMTHLQSYSLPPEQLSSISQGAVPSSANQALPSQQTQASYPNAMVSSVQGNSYPSQASIYSPPAAAAAAA
AAAVVPVPVPADVTIYQNAGPTMSQVPNYTLTSSTLPQTGGSQQPPQPQQAYSQKALL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011484
ORF Size 1644 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011484.3
RefSeq Size 3720 bp
RefSeq ORF 1647 bp
Locus ID 20844
UniProt ID P70297
Cytogenetics 2 A1-A2
Summary Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_011484" in other vectors (4)
SKU Description Size Price
MC218776 Stam (untagged) - Mouse signal transducing adaptor molecule (SH3 domain and ITAM motif) 1 (Stam), (10ug) 10 ug
$562.00
MR208719 Stam (Myc-DDK-tagged) - Mouse signal transducing adaptor molecule (SH3 domain and ITAM motif) 1 (Stam) 10 ug
$289.00 $511.00
MR208719L3 Lenti ORF clone of Stam (Myc-DDK-tagged) - Mouse signal transducing adaptor molecule (SH3 domain and ITAM motif) 1 (Stam) 10 ug
$811.00
MR208719L4 Lenti ORF clone of Stam (mGFP-tagged) - Mouse signal transducing adaptor molecule (SH3 domain and ITAM motif) 1 (Stam) 10 ug
$811.00

Plasmid Map for MG208719

Download sequence for

Cloning scheme for MG208719

Circular map for MG208719

file-alt Citations

Proteomic analyses of signalling complexes associated with receptor tyrosine kinase identify novel members of fibroblast growth factor receptor 3 interactome
Balek, Nemec, Konik et al
Cell. Signal. (2017)
AI Summary: In this study, OriGene products—specifically vectors expressing STAM1, SHOX2, and EEF1A1—were used to facilitate the identification and validation of novel protein interactions within the FGFR3 signalling complex.
Snippet: …Vectors expressing STAM1, SHOX2 and EEF1A1 were obtained from Origene (San Diego, CA). …Vectors expressing STAM1, SHOX2 and EEF1A1 were obtained from Origene (San Diego, CA). …
The Dual Role of an ESCRT-0 Component HGS in HBV Transcription and Naked Capsid Secretion
Chou, Tsai, Huang
PLOS pathogens (2015) 11 (10)
AI Summary: OriGene contributed Myc-Flag-tagged HGS, STAM1, and STAM2 expression vectors to the study, enabling the investigation of these ESCRT-0 components in HBV transcription and naked capsid secretion.
Snippet: …The Myc-Flag-tagged HGS, STAM1, and STAM2 expression vectors used in the study were all purchased from OriGene. Plasmid HGS dUIM was derived from the wild type HGS backbone by deleting a putative ubiquitin-interaction motif at amino acid 257–277 by PCR amplification. …
Showing 1-2 of 2 papers.
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