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Elp3 (NM_028811) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG208702
Elp3 (tGFP-tagged) - Mouse elongation protein 3 homolog (S. cerevisiae) (Elp3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $709.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Elp3
Synonyms 2610507P14Rik; KAT9
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG208702 representing NM_028811
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGCAAAAGAGGAAAGGGGACCTCAGCCCGGCTGAGCTGATGATGCTGACTATAGGAGACGTTATCA
AGCAGCTGGTTGAAGCTCACGAGCAGGGGAAAGACGTCGATCTAAATAAGATGAAAACCAAGACAGCTGC
CAAGTATGGCCTTGCATCCCAGCCCCGCCTGGTGGATATCATCGCTGCAGTCCCTCCTCACTATCGAAAG
ATCTTGATTCCAAAGCTAAAGGCGAAGCCCGTCAGGACTGCCAGTGGGATCGCTGTTGTGGCTGTGATGT
GCAAACCCCACAGATGTCCACACATCAGTTTCACAGGGAATATATGTATATACTGCCCCGGAGGACCTGA
CTCCGATTTTGAGTATTCAACCCAGTCTTACACGGGCTATGAGCCAACTTCCATGAGAGCTATCCGTGCT
AGATATGACCCTTTTCTGCAGACAAGACACCGGATAGAGCAGCTAAAGCAGCTTGGTCACAGTGTGGATA
AGGTGGAGTTCATCGTGATGGGTGGAACGTTCATGGCCCTTCCAGAAGAGTACAGAGATTATTTCATCCG
AAGTTTACACGATGCTTTATCAGGCCATACTTCAAACAATATTCACGAGGCAATCAAGTATTCTGAGAGA
AGCTTCACAAAGTGTGTTGGAATTACAATTGAGACCAGACCTGATTATTGCATGAAGCGGCACCTAAGTG
ACATGTTGACTTATGGCTGCACAAGGCTGGAGATCGGAGTGCAGAGTGTCTATGAAGATGTGGCCAGAGA
TACCAACAGGGGCCATACTGTGAAGGCAGCGTGTGAATCTTTCCACCTGGCCAAAGATTCTGGTTTCAAA
GTGGTGACGCATATGATGCCTGACCTGCCGAACGTGGGACTGGAGAGAGACATTGAGCAGTTTATAGAAT
TTTTCGAGAACCCTGCTTTTCGTCCTGATGGTTTGAAGCTCTACCCAACCCTGGTTATCCGTGGAACTGG
TCTGTATGAGCTGTGGAAATCAGGAAGATACAGGAGCTATTCTCCCAGTGACCTGATTGAGCTGGTGGCT
CGGATCCTAGCCCTTGTGCCCCCGTGGACTCGTGTGTACCGGGTGCAGAGAGACATCCCAATGCCCCTTG
TCAGCTCAGGAGTGGAGCATGGCAACCTGCGAGAGTTGGCATTTGCAAGAATGAAGGATCTTGGAATACA
GTGCCGTGATGTGAGAACCAGGGAGGTTGGAATCCAAGAAATTCATCACAGAGTGCGGCCATACCAGGTC
GAGTTGGTACGGAGAGATTATGTTGCCAATGGTGGCTGGGAAACTTTCCTGTCCTACGAAGATCCTGATC
AGGACATTCTAATTGGCCTCCTAAGGCTGCGGAAGTGCTCTGAGGAAACCTTCCGGTTTGAACTGGGTGG
AGGTGTTTCCATAGTCCGAGAGCTACACGTGTATGGAAGTGTGGTCCCTGTGAGCAGCCGGGATCCCACT
AAATTTCAGCATCAGGGGTTCGGCATGCTGCTGATGGAAGAGGCAGAGAGAATCGCTCGGGAAGAGCATG
GATCTGGGAAAATGGCAGTTATATCAGGGGTCGGCACAAGAAATTACTACCGAAAGATTGGCTACAGATT
ACAAGGCCCCTATATGGTAAAGATGCTAAAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG208702 representing NM_028811
Red=Cloning site Green=Tags(s)

MRQKRKGDLSPAELMMLTIGDVIKQLVEAHEQGKDVDLNKMKTKTAAKYGLASQPRLVDIIAAVPPHYRK
ILIPKLKAKPVRTASGIAVVAVMCKPHRCPHISFTGNICIYCPGGPDSDFEYSTQSYTGYEPTSMRAIRA
RYDPFLQTRHRIEQLKQLGHSVDKVEFIVMGGTFMALPEEYRDYFIRSLHDALSGHTSNNIHEAIKYSER
SFTKCVGITIETRPDYCMKRHLSDMLTYGCTRLEIGVQSVYEDVARDTNRGHTVKAACESFHLAKDSGFK
VVTHMMPDLPNVGLERDIEQFIEFFENPAFRPDGLKLYPTLVIRGTGLYELWKSGRYRSYSPSDLIELVA
RILALVPPWTRVYRVQRDIPMPLVSSGVEHGNLRELAFARMKDLGIQCRDVRTREVGIQEIHHRVRPYQV
ELVRRDYVANGGWETFLSYEDPDQDILIGLLRLRKCSEETFRFELGGGVSIVRELHVYGSVVPVSSRDPT
KFQHQGFGMLLMEEAERIAREEHGSGKMAVISGVGTRNYYRKIGYRLQGPYMVKMLK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028811
ORF Size 1641 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028811.1, NP_083087.1
RefSeq Size 2724 bp
RefSeq ORF 1644 bp
Locus ID 74195
UniProt ID Q9CZX0
Cytogenetics 14 D1
Summary Catalytic histone acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4. Involved in acetylation of alpha-tubulin (By similarity). May also have a methyltransferase activity. Involved in cell migration. Involved in neurogenesis. Regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation (PubMed:19185337).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_028811" in other vectors (4)
SKU Description Size Price
MC205564 Elp3 (untagged) - Mouse elongation protein 3 homolog (S. cerevisiae) (Elp3), (10ug) 10 ug
$510.00
MR208702 Elp3 (Myc-DDK-tagged) - Mouse elongation protein 3 homolog (S. cerevisiae) (Elp3) 10 ug
$289.00 $509.00
MR208702L3 Lenti ORF clone of Elp3 (Myc-DDK-tagged) - Mouse elongation protein 3 homolog (S. cerevisiae) (Elp3) 10 ug
$809.00
MR208702L4 Lenti ORF clone of Elp3 (mGFP-tagged) - Mouse elongation protein 3 homolog (S. cerevisiae) (Elp3) 10 ug
$809.00

Plasmid Map for MG208702

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG208702
Circular map for MG208702

file-alt Citations

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