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Wdr20a (BC026761) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG208616
Wdr20a (tGFP-tagged) - Mouse WD repeat domain 20a (cDNA clone MGC:30262 IMAGE:4221717)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $702.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Wdr20a
Synonyms WDR20
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG208616 representing BC026761
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGACGGAGGGAGGAGGGAAGGAGATGAACGAGATTAAGACCCAATTCACCACCCGGGAAGGTCTGT
ACAAGCTGCTGCAGCACTCGGAGTACAGCCGGCCCAACCGGGTGCCCTTCAACTCGCAGGGATCCAACCC
TGTCCGCGTCTCCTTCGTAAACCTCAACGACCAGTCTGGCAACGGCGACCGCCTCTGCTTCAATGTGGGC
CGGGAGCTCTACTTCTATATCTACAAGGGGGTCCGCAAGGCTGCCGACCTGAGTAAACCCATAGATAAGA
GGATATACAAAGGGACGCAGCCCACTTGCCATGACTTCAACCTCCTCACAGCTACAGCAGAGAGCGTCTC
TCTCCTAGTGGGCTTTTCCGCAGGCCAAGTCCAGCTTATAGACCCAATCAAGAAAGAGACCAGCAAGCTG
TTTAATGAGGAAAGACTAATAGACAAGTCCCGCGTAACCTGTGTCAAATGGGTTCCTGGTTCGGAAAGCC
TTTTCCTAGTAGCCCATTCAAGTGGGAACATGTACTTATATAATGTGGAGCACACTTGTGGCACCACAGC
CCCCCACTACCAGCTCCTGAAGCAGGGAGAAAGCTTTGCCGTGCACACTTGCAAGAGCAAATCCACGAGG
AACCCTCTCCTTAAGTGGACGGTGGGCGAGGGGGCCCTCAACGAGTTTGCTTTCTCCCCAGATGGCAAGT
TCTTAGCGTGTGTGAGCCAGGACGGGTTTCTGCGTGTGTTCAACTTTGACTCAGTGGAGCTGCACGGTAC
AATGAAAAGCTACTTTGGGGGCTTGCTTTGTGTGTGCTGGAGCCCAGATGGCAAGTACATTGTGACAGGT
GGAGAGGACGACTTGGTGACAGTCTGGTCCTTTCTAGACTGCCGAGTAATAGCTAGAGGCCATGGGCACA
AATCCTGGGTCAGTGTTGTAGCATTTGATCCCTATACTACTAGTGTAGAAGAAAGTGACCCTATGGAGTT
TAGTGGCAGTGACGAGGACTTCCAGGACCTTCTTCATTTTGGCAGAGATCGAGCGAACAGTACACAGTCT
AGGCTATCCAAACGGAACTCTACAGACAGTCGCCCTCCCCTCTCAAGAGCAAGGACACATACAAACGTTA
TGAATGCCACAAGTCCGCCTGCCGGAAGCAATGGGAACAGTGTCACTACACCTGGGAACTCTGTGCCCCC
TCCATTGCCACGGTCCAATAGCCTTCCACATTCAGCAGTCTCCAATGCTGCTAGCAAAGGCAGCGTCATG
GACGGGGCCATTGCCTCCGGGGTCAGCAAGTTTGCAACTCTGTCGCTACATGACCGGAAGGAGAGACACC
ACGAGAAAGACCATAAACGAAACCATAGTATGGGACACATTTCCAGCAAGAGCAGTGATAAACTGAACCT
AGTTACTAAAGCCAAAACGGACCCAGCTAAAACTCTGGGGACATCCCTGTGTCCTCGGATGGAAGATGTT
CCCTTGTTAGAGCCACTGATCTGTAAAAAGATAGCTCATGAAAGACTGACGGTATTGGTTTTTCTTGAAG
ACTGTATAGTCACTGCTTGTCAGGAGGGATTTATTTGCACATGGGCAAGGCCCGGTAAAGTGGTAAGTTT
TAATCCT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG208616 representing BC026761
Red=Cloning site Green=Tags(s)

MATEGGGKEMNEIKTQFTTREGLYKLLQHSEYSRPNRVPFNSQGSNPVRVSFVNLNDQSGNGDRLCFNVG
RELYFYIYKGVRKAADLSKPIDKRIYKGTQPTCHDFNLLTATAESVSLLVGFSAGQVQLIDPIKKETSKL
FNEERLIDKSRVTCVKWVPGSESLFLVAHSSGNMYLYNVEHTCGTTAPHYQLLKQGESFAVHTCKSKSTR
NPLLKWTVGEGALNEFAFSPDGKFLACVSQDGFLRVFNFDSVELHGTMKSYFGGLLCVCWSPDGKYIVTG
GEDDLVTVWSFLDCRVIARGHGHKSWVSVVAFDPYTTSVEESDPMEFSGSDEDFQDLLHFGRDRANSTQS
RLSKRNSTDSRPPLSRARTHTNVMNATSPPAGSNGNSVTTPGNSVPPPLPRSNSLPHSAVSNAASKGSVM
DGAIASGVSKFATLSLHDRKERHHEKDHKRNHSMGHISSKSSDKLNLVTKAKTDPAKTLGTSLCPRMEDV
PLLEPLICKKIAHERLTVLVFLEDCIVTACQEGFICTWARPGKVVSFNP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC026761
ORF Size 1617 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC026761, AAH26761
RefSeq Size 2290 bp
RefSeq ORF 1619 bp
Locus ID 69641
Cytogenetics 12 F1
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"BC026761" in other vectors (4)
SKU Description Size Price
MC217866 Wdr20a (untagged) - Mouse WD repeat domain 20a (cDNA clone MGC:30262 IMAGE:4221717), (10ug) 10 ug
$553.00
MR208616 Wdr20a (Myc-DDK-tagged) - Mouse WD repeat domain 20a (cDNA clone MGC:30262 IMAGE:4221717) 10 ug
$289.00 $502.00
MR208616L3 Lenti ORF clone of Wdr20a (Myc-DDK-tagged) - Mouse WD repeat domain 20a (cDNA clone MGC:30262 IMAGE:4221717) 10 ug
$802.00
MR208616L4 Lenti ORF clone of Wdr20a (mGFP-tagged) - Mouse WD repeat domain 20a (cDNA clone MGC:30262 IMAGE:4221717) 10 ug
$802.00

Plasmid Map for MG208616

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG208616
Circular map for MG208616

file-alt Citations

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