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Dalrd3 (BC013483) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG208602
Dalrd3 (tGFP-tagged) - Mouse DALR anticodon binding domain containing 3 (cDNA clone MGC:18914 IMAGE:4242135)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $701.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dalrd3
Synonyms 6330580J24Rik; C77829; mir-425
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG208602 representing BC013483
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGACCGGGCGCCTTGGGGTTGGCGAGACGCTGGAGGCCCTTAATGCAGCTGTAGGGCCTGGTAGCC
CAGTGTGGTTTAAGGAAACCCATGCCCGGCACCTTCGTGTTCGAGACTTCTTGGCACCGCGAAGCGCGCT
ACAGGCGCGCTTCAGGGACGGCCAGGTGCCAGAGTGCGTGTTCCGTGCGGTCTCCTGCCTGCAGGGTCCC
GGAGTGGCCCCTGTACTGCGCTGCGCGCCGACACCCGCGGGTTTATCGCTCCAACTACAGCGGCCCGCTG
TCTTCGAACATGTACTCGGTGCCCTGGCCTCCTATGCTACTCCCGCCAAACCTGCCTCACCAGGCCCGCG
AGTTGTCCTACACTGCCCAGCACTGCGCTGTAATTCGGGGACGCTCCGATTGAGCCAGCTGCGTGCCGTG
CTCGTAGCCGATCATCTAGTACGGGTACTGCGTGCTCATGGGGTACGTGTGTGCTCAGTGCCCCCGGTGC
GGGACCCTCACATGTCAACTTTCCTCCAGAAACTTCGGGTGGAATGGCCCACTGCCTCGAAGAGCACCTC
GACCGAAACACTGAGAACCTGTGTTCTTGCAAACCTTAATGGTTCTAAGGAAGCGACGCTGCCCCCTGGC
GTCCTAGGCCGACTGTGTCTGAAGGAACTGGTAGAACAGAGAGGCACTGCTGGCTATGACCCCAGCATAG
ACCACTGTTTGGTGACTGAAGATGTGCTCTCTGTGCTCTCGGAGCTGCAGGAGGCTGTGCGCCACTGGCC
AGAGGGCGGCCACCCAGGCCCGGCTGGGCGTCCAGATGCTGGTGTAGATGACTGCGTGGTTATACACGTG
GTTAGCTGTGAAGAGGCATTCCAGCAACAAAAGCTGGATTTGCTCTGGCAGAAATTGGATGACCGGGCTC
CTCACAAGCAGAAACACCTGGTGTGTGGTCCCGTGAAAATGGCTGGTGTACCCGGCACCCAGCTGACTGC
CCCTCAGTACTACAGGCTCAGACATGCTCAGGTGTGCGAGGCCTCAGCTCTGAAGCACGGAGGAGACCTG
GCACAAGACCCCGCCTGGACTGAGACCTTCGACATCCTCTCCGTGGCTACCATCAAATTTGAGATGCTAA
GCACAGCCCCACAGAGCCAGCTCCTCCTGGCCCACAGCACCATCTCAACAAAGGGCACAAAGAGCGGGAC
TTTTGTCATGTATAACTGTGCCCGCCTTGCCACACTCTTCGAGGGCTACAAACATGGAACGGAGCAAGGT
CTGTATCCCACTTTTCCGCTTGTGAGCAGTCTGGACTTCTCACTACTCCATGATGAGGGTGAGTGGCTGC
TGCTTTTCAACAGTGTCCTTCCCTTTCTGGACCTGCTGAGCCAGACTGTGAGTCTGGCCGGCACCCCAGG
CCTCCACATCCCTGTCCGCACGGAGATGGTATGCAAGTTCCTGGTGCAGCTCAGCATGGATTTCAGCTCC
TACTATAACCGAGTACATATTCTAGGGGAACCTCGGCCACACCTCTTTGGTCAGATGTTTGCTCGCCTAC
AGCTTTTGAGGGCAGTGCGTGAGGTGTTCCACACAGGCTTGGCTATGCTGGGCCTCCCTCCGCTGAGCCA
CATC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG208602 representing BC013483
Red=Cloning site Green=Tags(s)

MATGRLGVGETLEALNAAVGPGSPVWFKETHARHLRVRDFLAPRSALQARFRDGQVPECVFRAVSCLQGP
GVAPVLRCAPTPAGLSLQLQRPAVFEHVLGALASYATPAKPASPGPRVVLHCPALRCNSGTLRLSQLRAV
LVADHLVRVLRAHGVRVCSVPPVRDPHMSTFLQKLRVEWPTASKSTSTETLRTCVLANLNGSKEATLPPG
VLGRLCLKELVEQRGTAGYDPSIDHCLVTEDVLSVLSELQEAVRHWPEGGHPGPAGRPDAGVDDCVVIHV
VSCEEAFQQQKLDLLWQKLDDRAPHKQKHLVCGPVKMAGVPGTQLTAPQYYRLRHAQVCEASALKHGGDL
AQDPAWTETFDILSVATIKFEMLSTAPQSQLLLAHSTISTKGTKSGTFVMYNCARLATLFEGYKHGTEQG
LYPTFPLVSSLDFSLLHDEGEWLLLFNSVLPFLDLLSQTVSLAGTPGLHIPVRTEMVCKFLVQLSMDFSS
YYNRVHILGEPRPHLFGQMFARLQLLRAVREVFHTGLAMLGLPPLSHI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC013483
ORF Size 1614 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC013483.1
RefSeq Size 1736 bp
RefSeq ORF 1616 bp
Locus ID 67789
Cytogenetics 9 F2
Summary Involved in tRNA methylation. Facilitates the recognition and targeting of tRNA(Arg)(CCU) and tRNA(Arg)(UCU) substrates for N(3)-methylcytidine modification by METTL2.UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC013483" in other vectors (3)
SKU Description Size Price
MR208602 Dalrd3 (Myc-DDK-tagged) - Mouse DALR anticodon binding domain containing 3 (cDNA clone MGC:18914 IMAGE:4242135) 10 ug
$289.00 $501.00
MR208602L3 Lenti ORF clone of Dalrd3 (Myc-DDK-tagged) - Mouse DALR anticodon binding domain containing 3 (cDNA clone MGC:18914 IMAGE:4242135) 10 ug
$801.00
MR208602L4 Lenti ORF clone of Dalrd3 (mGFP-tagged) - Mouse DALR anticodon binding domain containing 3 (cDNA clone MGC:18914 IMAGE:4242135) 10 ug
$801.00

Plasmid Map for MG208602

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG208602
Circular map for MG208602

file-alt Citations

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