Stxbp4 (BC032881) Mouse Tagged ORF Clone

SKU MG208536

Stxbp4 (tGFP-tagged) - Mouse syntaxin binding protein 4 (cDNA clone MGC:41138 IMAGE:1224878)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Stxbp4
• Synonyms: 6030470M02Rik; Synip
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208536 representing BC032881; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGTGATGGTACAGCTTCTGCCCGATCATCCAGCCCACTTGACAGGGATCCTGCCTTTCGGGTGATTA; CAGTTACTAAGGAAACAGGTCTAGGTCTGAAGATCCTAGGCGGAATTAACAGGAATGAAGGACCGCTGGT; GTATATTCACGAAGTCATTCCTGGAGGTGACTGTTACAAGGATGGACGTTTGAAGCCAGGAGATCAACTT; GTCTCAATAAACAAGGAATCTATGATTGGTGTATCATTTGAAGAAGCAAAAAGCATAATTACCAGAGCCA; AGTTGAGGTCAGAATCTCCCTGGGAGATAGCATTCATCAGACAAAAGTCTTACTGTGGCCATCCAGGAAA; TATTTGCTGTCCATCCCCACAAGTGTCAGAAGACTGTGGACCTCAAACCTCAACATTTACTCTTCTTTCC; TCTCCCTCTGAAACACTACTTCCAAAGACTTCATCCACTCCCCAGACTCAGGACTCCACTTTCCCTTCTT; GTAAAGCAATTCAGACAAAACCTGAACACGATAAAACAGAACATAGTCCAATTACTTCTTTGGACAACAG; CCCTGCAGATACATCTAATGCAGACATTGCTCCAGCCTGGACTGATGATGATTCTGGACCACAAGGAAAG; ATTTCCCTAAATCCTTCTGTTCGCCTTAAGGCAGAGAAACTGGAAATGGCTCTCAATTACCTCGGTATAC; AGCCAACAAAGGAACAACGTGAAGCCCTGAGAGAGCAAGTCCAGGCCGACTCAAAGGGGACTGTGTCTTT; TGGAGATTTCGTCCAGGTTGCCAGAAGTTTGTTTTGCTTGCAGTTGGATGAAGTAAATGTTGGTGTCCAT; GAAATCCCCAGCATCTTAGACTCACAGCTTCTCCCCTGTGATTCTCTAGAAGCAGATGAAGTGGGAAAAC; TTAGACAAGAAAGAAACGCTGCTCTAGAGGAACGGAATGTGCTTAAGGAGAAGTTACTGGAATCAGAAAA; GCACAGGAAACAATTGATAGAAGAACTCCAGAATGTGAAGCAGGAAGCCAAAGCTGTAGCTGAGGAAACC; CGAGCTCTGCGAAGCCGGATTCATCTCGCAGAAGCTGCACAGCGGCAGGCACACGGGATGGAAATGGATT; ATGAAGAGGTGATCCGTCTGCTAGAGGCTGAGGTCTCAGAACTAAAGGCTCAGCTCGCTGATTATTCTGA; CCAAAATAAAGAAAGTGTCCAGGACTTGAGAAAAAGAGTCACCGTTCTTGACTGCCAATTGCGAAAATCA; GAAATGGCTCGGAAAGCATTCAAGGCGTCCACTGAAAGGCTCCTTGGTTTCATAGAGGCTATTCAAGAAG; TTCTTTTGGATAGTTCTGCTCCTTTATCAACTTTAAGTGAAAGAAGAGCTGTGCTCGCATCTCAGACTTC; GCTTCCACTGCTGGCAAGGAACGGACGCAGCTTCCCAGCAACCCTGCTTCTGGAATCCAAGGAGCTTGTC; AGATCTGTCCGTGCCATACTTGATATGGACTGTAAGTTATCATTATCATCTTCTTCTTCTCCTTCCTCCT; CCTCCTCCTTCTCCTCCTCTTCTTCTTGTAATTTATTAAACAAAGCAGAAGAAAGACCTT; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208536 representing BC032881; Red=Cloning site Green=Tags(s); ; MSDGTASARSSSPLDRDPAFRVITVTKETGLGLKILGGINRNEGPLVYIHEVIPGGDCYKDGRLKPGDQL; VSINKESMIGVSFEEAKSIITRAKLRSESPWEIAFIRQKSYCGHPGNICCPSPQVSEDCGPQTSTFTLLS; SPSETLLPKTSSTPQTQDSTFPSCKAIQTKPEHDKTEHSPITSLDNSPADTSNADIAPAWTDDDSGPQGK; ISLNPSVRLKAEKLEMALNYLGIQPTKEQREALREQVQADSKGTVSFGDFVQVARSLFCLQLDEVNVGVH; EIPSILDSQLLPCDSLEADEVGKLRQERNAALEERNVLKEKLLESEKHRKQLIEELQNVKQEAKAVAEET; RALRSRIHLAEAAQRQAHGMEMDYEEVIRLLEAEVSELKAQLADYSDQNKESVQDLRKRVTVLDCQLRKS; EMARKAFKASTERLLGFIEAIQEVLLDSSAPLSTLSERRAVLASQTSLPLLARNGRSFPATLLLESKELV; RSVRAILDMDCKLSLSSSSSPSSSSSFSSSSSCNLLNKAEERP; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC032881
• ORF Size: 1600 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC032881, AAH32881
• RefSeq Size: 1807 bp
• RefSeq ORF: 1601 bp
• Locus ID: 20913
• Cytogenetics: 11 D
• Summary: Plays a role in the translocation of transport vesicles from the cytoplasm to the plasma membrane. Inhibits the translocation of SLC2A4 from intracellular vesicles to the plasma membrane by STX4A binding and preventing the interaction between STX4A and VAMP2. Stimulation with insulin disrupts the interaction with STX4A, leading to increased levels of SLC2A4 at the plasma membrane. May also play a role in the regulation of insulin release by pancreatic beta cells after stimulation by glucose.[UniProtKB/Swiss-Prot Function]