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Wrap53 (NM_144824) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG208531
Wrap53 (tGFP-tagged) - Mouse WD repeat domain 79 (Wdr79)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
$489.00 $696.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Wrap53
Synonyms BC021790; Wdr79
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG208531 representing NM_144824
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGACGTCGGAGGAGCGACTCTTAGCTCCGGATAGCCTCCCTCCTGACCTGGCCCCAGCTCCGGTAC
CCCAAGGTTCCCCAGCGGAGAAAAATACAGACTTTGAACCGGTGCCTCCGCCATGCGGTGGTGATGACCA
ACCCCAGCTAGCCACAGATCCCGTGGCTAGCTTGGTTGTGTCCCAGGAGCTTCAACAAGGGGACTCTGTA
CCTCTGGAAGTAGAATTTAATACTTCCAGTGAGTTGAGTCCTGGAATCGAGGAGCAAGATGTTTCTGAAC
ATGCGAGTCTTCCCGGAGAAGAAACGAATCTCCCCGAGTTGGAGTCAGGAGAAGCCACGGAAGGTGTGTC
TGAGGAACGCGCTGAAGTGGATGAGGGGGACACCTTTTGGACCTACAGCTTCTCGCAGGTACCCCGATAC
CTCAGTGGTTCCTGGTCCGAGTTCAGCACCCGCTCCGAGAACTTCTTGAAAGGTTGCAAGTGGGCCCCTG
ACGGTTCCTGTATCTTGACCAATAGTGCTGATAATGTCCTTCGGATTTACAACCTGCCCCCAGAATTGTA
CAGTGAACAAGAGCAGGTGGATTACGCCGAAATGGTTCCTGTCCTTCGAATGGTGGAGGGCGACACCATC
TATGATTACTGCTGGTATTCTCTGATGTCCTCCACCCAGCCAGACACCTCCTATGTGGCCAGCAGCAGCC
GGGAGAACCCGATTCACATCTGGGATGCATTCACTGGAGAACTACGGGCTTCCTTTCGCGCTTACAACCA
CCTGGATGAGTTGACAGCAGCCCACTCGCTCTGTTTCTCCCCGGATGGTTCCCAACTCTTCTGTGGCTTC
AACCGGACTGTGCGTGTCTTTTCCACATCCAGGCCTGGCAGAGACTGTGAGGTGCGAGCCACATTTGCCA
AAAAGCAGGGCCAGAGCGGTATCATCTCCTGTATAGCCTTCAGTCCATCCCAGCCCCTCTATGCCTGTGG
CTCCTACGGCCGCACCATAGGCCTGTATGCCTGGGATGATGGTTCGCCTCTTGCCTTGCTGGGAGGCCAC
CAAGGGGGTATCACCCACCTTTGCTTTCACCCTGATGGCAATCTCTTCTTCTCGGGAGCCCGAAAGGATG
CTGAACTTCTGTGCTGGGATCTCCGGCAGCCAGGCCACCTCCTGTGGTCTCTGAGTCGGGAAGTGACCAC
TAATCAGCGCATCTACTTTGATCTGGACCCGAGCGGTCAGTTCCTAGTGAGCGGCAACACCAGTGGAGTT
GTCTCTGTGTGGGATATCAGTGGAGCTCTTAGTGACGACAGCAAGCTGGAACCCGTGGTGACTTTTCTGC
CTCAGAAGGACTGCACCAACGGAGTCAGCCTTCACCCTACCTTGCCTCTACTGGCCACTGCTTCTGGCCA
GCGCGTGTTCCCTGAGCCCACCAATAGCGGGGATGAAGGTGAGCTGGAGCTGGAGCTTCCTCTGCTCTCC
CTGTGCCACGCCCATCCCGAATGTCAGCTTCAGCTCTGGTGGTGTGGAGGGGGACCTGACCCCAGTAGCC
CTGTTGACGACCAGGATGAAAAGGGACAAAGAAGGACAGAGGCTGTGGGGATGAGT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG208531 representing NM_144824
Red=Cloning site Green=Tags(s)

MKTSEERLLAPDSLPPDLAPAPVPQGSPAEKNTDFEPVPPPCGGDDQPQLATDPVASLVVSQELQQGDSV
PLEVEFNTSSELSPGIEEQDVSEHASLPGEETNLPELESGEATEGVSEERAEVDEGDTFWTYSFSQVPRY
LSGSWSEFSTRSENFLKGCKWAPDGSCILTNSADNVLRIYNLPPELYSEQEQVDYAEMVPVLRMVEGDTI
YDYCWYSLMSSTQPDTSYVASSSRENPIHIWDAFTGELRASFRAYNHLDELTAAHSLCFSPDGSQLFCGF
NRTVRVFSTSRPGRDCEVRATFAKKQGQSGIISCIAFSPSQPLYACGSYGRTIGLYAWDDGSPLALLGGH
QGGITHLCFHPDGNLFFSGARKDAELLCWDLRQPGHLLWSLSREVTTNQRIYFDLDPSGQFLVSGNTSGV
VSVWDISGALSDDSKLEPVVTFLPQKDCTNGVSLHPTLPLLATASGQRVFPEPTNSGDEGELELELPLLS
LCHAHPECQLQLWWCGGGPDPSSPVDDQDEKGQRRTEAVGMS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_144824
ORF Size 1596 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_144824.1
RefSeq Size 1896 bp
RefSeq ORF 1599 bp
Locus ID 216853
UniProt ID Q8VC51
Cytogenetics 11 B3
Summary RNA chaperone that plays a key role in telomere maintenance and RNA localization to Cajal bodies (PubMed:29804836). Specifically recognizes and binds the Cajal body box (CAB box) present in both small Cajal body RNAs (scaRNAs) and telomerase RNA template component (TERC) (PubMed:29804836). Essential component of the telomerase holoenzyme complex, a ribonucleoprotein complex essential for the replication of chromosome termini that elongates telomeres in most eukaryotes (By similarity). In the telomerase holoenzyme complex, required to stimulate the catalytic activity of the complex (PubMed:29804836). Acts by specifically binding the CAB box of the TERC RNA and controlling the folding of the CR4/CR5 region of the TERC RNA, a critical step for telomerase activity (By similarity). In addition, also controls telomerase holoenzyme complex localization to Cajal body (By similarity). During S phase, required for delivery of TERC to telomeres during S phase and for telomerase activity (By similarity). In addition to its role in telomere maintenance, also required for Cajal body formation, probably by mediating localization of scaRNAs to Cajal bodies (By similarity). Also plays a role in DNA repair: phosphorylated by ATM in response to DNA damage and relocalizes to sites of DNA double-strand breaks to promote the repair of DNA double-strand breaks (By similarity). Acts by recruiting the ubiquitin ligase RNF8 to DNA breaks and promote both homologous recombination (HR) and non-homologous end joining (NHEJ) (By similarity).UniProtKB/Swiss-Prot Function
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"NM_144824" in other vectors (3)
SKU Description Size Price
MR208531 Wrap53 (Myc-DDK-tagged) - Mouse WD repeat containing, antisense to TP53 (Wrap53) 10 ug
$289.00 $496.00
MR208531L3 Lenti ORF clone of Wrap53 (Myc-DDK-tagged) - Mouse WD repeat containing, antisense to TP53 (Wrap53) 10 ug
$796.00
MR208531L4 Lenti ORF clone of Wrap53 (mGFP-tagged) - Mouse WD repeat containing, antisense to TP53 (Wrap53) 10 ug
$796.00

Plasmid Map for MG208531

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Cloning scheme for MG208531
Circular map for MG208531

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