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Parp8 (BC021315) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG208030
Parp8 (tGFP-tagged) - Mouse poly (ADP-ribose) polymerase family, member 8 (cDNA clone MGC:29205 IMAGE:5027417)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Parp8 or BC021315"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Parp8
Synonyms 2810430O08Rik; ARTD16; D13Ertd275e
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG208030 representing BC021315
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCACCATTAAATCGCACAAACTTTTGGGCCGGCCCTGCCCCTCAGCCGGCAAGCAAGAGGACTGCC
TCACACTGAAGTCCCATAAGTTATTGACTCGATCTTGTTCTGGAGACCCACGATGTGAGCACAACACCAA
CTTGAAGCCCCACAAACTGCTAAGCAGATCTTACTCCAGTAATCTCAGAATGGAAGAGTTATATGGCCTG
AAAAATCACAAATTGCTCAGCAAGTCTTACTCCAGTGCCCCCAAGACATCGAAAATGGAGCATTTCAAGG
AACCCAACGCAGAGGGCAGGAGGCTCTCGCTTACCTCAGGGCTTATCGGTATTTTAACACCATCATCGTC
TTCTTCCCAGCCTCCTACAAATGGTGCAAAAAGCATTCCAATCCGAGACCGCGGCTTCCTGGTGCAGACC
ATTGAGTTTGCTGAGCAGCGGATCCCTGTACTGAATGAATATTGTGTGGTGTGTGATGAACCACACGTGT
TTCAAAATGGCCCCATGCTTAGGCCCACAGTATGTGAGCGAGAGCTGTGTGTGTTTGCTTTTCAAACCCT
GGGAGTGATGAATGAAGCTGCCGACGAGATCGCTACCGGAGCTCAGGTGGTAGATCTGCTTGTGTCCATG
TGTAGATCTGCGTTGGAATCTCCTAGAAAAGTTGTCATTTTTGAGCCATATCCTTCTGTGGTAGATCCTA
ATGACCCTCAGATGTTAGCCTTCAATCCCAGGAAGAAGAACTATGATCGTGTAATGAAAGCTCTGGACAG
CATAACTTCCATCAGAGAAATGACACAGGCACCCTATCTGGAAATCAAGAAGCAGATGGATAAACAGGAC
CCCCTTGCTCATCCACTTCTGCAATGGGTCATCTCAAGTAATAGGTCACATATTGTGAAACTGCCAGTCA
ACAGGCAACTGAAGTTCATGCACACGCCACACCAGTTCCTCCTCCTCAGCAGCCCACCAGCCAAAGAGTC
CAACTTCAGAGCCGCCAAGAAGCTCTTCGGGAGCACCTTCGCATTTCATGGCTCTCACATTGAAAACTGG
CACTCTATCCTGAGGAATGGTCTGGTCGTTGCCTCTAACACGCGTCTGCAGCTCCACGGTGCAATGTACG
GAAGTGGGATCTATCTTAGCCCGATGTCCAGCATATCATTTGGTTACTCAGGGATGAACAAGAAGCAGAA
GGTGTCAAGCAAGGACGAGCCAGCTTCCAGTAGTAAAAGCAGCAATGCATCACAGTCTCAGAAAAAAGGA
CAGCAATCCCAGTTCCTGCAAAGCCGTAACTTGAAATGCATAGCCTTATGTGAAGTGATCACGTCACCTG
ACCTGCACAAACATGGCGAGATATGGGTTGTCCCGAACACGGATCATGTCTGTACAAGATTCTTTTTTGT
TTATGAAGATGGCCAGGTGGGAGATGCCAATATCAACACACAAGAAGGAGGTATTCACAAAGAGATACTC
CGTGTGATTGGTAATCAGACTGCGACTGGT

AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG208030 representing BC021315
Red=Cloning site Green=Tags(s)

MSTIKSHKLLGRPCPSAGKQEDCLTLKSHKLLTRSCSGDPRCEHNTNLKPHKLLSRSYSSNLRMEELYGL
KNHKLLSKSYSSAPKTSKMEHFKEPNAEGRRLSLTSGLIGILTPSSSSSQPPTNGAKSIPIRDRGFLVQT
IEFAEQRIPVLNEYCVVCDEPHVFQNGPMLRPTVCERELCVFAFQTLGVMNEAADEIATGAQVVDLLVSM
CRSALESPRKVVIFEPYPSVVDPNDPQMLAFNPRKKNYDRVMKALDSITSIREMTQAPYLEIKKQMDKQD
PLAHPLLQWVISSNRSHIVKLPVNRQLKFMHTPHQFLLLSSPPAKESNFRAAKKLFGSTFAFHGSHIENW
HSILRNGLVVASNTRLQLHGAMYGSGIYLSPMSSISFGYSGMNKKQKVSSKDEPASSSKSSNASQSQKKG
QQSQFLQSRNLKCIALCEVITSPDLHKHGEIWVVPNTDHVCTRFFFVYEDGQVGDANINTQEGGIHKEIL
RVIGNQTATG

SGPTRTRRLE - GFP Tag - V
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN BC021315
ORF Size 1500 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC021315, AAH21315
RefSeq Size 2143 bp
RefSeq ORF 1502 bp
Locus ID 52552
Cytogenetics 13 65.32 cM
Summary Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins.UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC021315" in other vectors (4)
SKU Description Size Price
MC217791 Parp8 (untagged) - Mouse poly (ADP-ribose) polymerase family, member 8 (cDNA clone MGC:29205 IMAGE:5027417), (10ug) 10 ug
$513.00
MR208030 Parp8 (Myc-DDK-tagged) - Mouse poly (ADP-ribose) polymerase family, member 8 (cDNA clone MGC:29205 IMAGE:5027417) 10 ug
$289.00 $457.00
MR208030L3 Lenti ORF clone of Parp8 (Myc-DDK-tagged) - Mouse poly (ADP-ribose) polymerase family, member 8 (cDNA clone MGC:29205 IMAGE:5027417) 10 ug
$757.00
MR208030L4 Lenti ORF clone of Parp8 (mGFP-tagged) - Mouse poly (ADP-ribose) polymerase family, member 8 (cDNA clone MGC:29205 IMAGE:5027417) 10 ug
$757.00

Plasmid Map for MG208030

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG208030
Circular map for MG208030

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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