Sfmbt1 (BC066188) Mouse Tagged ORF Clone

SKU MG207437

Sfmbt1 (tGFP-tagged) - Mouse Scm-like with four mbt domains 1 (cDNA clone MGC:76655 IMAGE:30476111)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Sfmbt1
• Synonyms: Sfmbt, Smr
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207437 representing BC066188; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGCGGGGAGCAGCAGCTTGATGCAGATCTTGGGTCTGGTGTAGAAGTAGAAGAATTCAGCTGGGAAG; ATTATCTAGAAGAGACAGGCTCCACCACAGTTCCCTACGCATCGTTTAAACATGTGGATATACGTTTGCA; AAATGGATTTGCTCCTGGGATGAAGCTGGAGGTAGCTCTGAAAAATGACCCTGAGACTTATTGGGTTGCC; ACCATCATCACCGCCTGTGAGCAGTTGCTCCTGCTTCGTTATGAAGGCTATGGGGAAGACCGAAAAGCAG; ACTTCTGGTGTGACATCAGGAAAGCTGGCCTCTACCCCATTGGGTGGTGTCAGCAGAACAAGAAGACCCT; CGAAGCTCCAGAAGGCATCAGAGATAAAGTGTCTGATTGGAATGCATTCCTACAGCAGACCCTGATAGGA; GCCTGTGGTCCTCCCGTGTCTCTGCTAGAGGGCCTCCGTAATGGAAGGAATCCTTTAGATCTCATTGCCC; CAGGGTCCAAACTAGAATGCCAGGATTTTCGGGACTCTTTAAGCACTTGGCTTGTTACTGTTGTGGAGAA; CATTGGGGGACGGTTGAAGCTGCGGTATGAAGGCCTTGAGAGCCGTGACGGTTTTGAACACTGGTTGTAT; TACTTAGATCCATTTCTTCATCACATTGGTTGGGCTGCTCAGCAAGGATGTGACCTTCAGCCCCCATTAG; CCATCAAACATCTGAAGAGTGAAGCTGACTGGCAAGAGATTTTGGCCAAAGTGAAAGAGGAAGAGCCATT; ACCCTCTTACTTATTTAAGGATAAGCAAGTTATTGGAACCCATGAATTTTCTATAAACATGAAGTTGGAA; GCTGTGGACCCGTGGTCTCCTTTTGGGATTTCTCCTGCAACGATTGCTAAGGTGTTTGATGATAAGTACT; TTCTAGTGGAAATGGATGACCTTCGGCCAGAAGACCATACAAGGCGATCATTTGTGTGCCATGCTAACAG; TCCTGGTATCTTTCCTGTGCAGTGGAGTCTAAAGAATGGTTTACATATCAATCCTCCTCCAGGCTTCCGG; AGCCAGGACTTTGACTGGGCCGACTACCTCAAGCAGTGTGGGGCTGAAGCTGCTCCCCAGAAGTGCTTCC; CGCAGTCAATTTCTGAGCACCAGTTTAAGGAGAACATGAAACTGGAGGCAGTGAACCCTCTTTTCCCTGA; AGAAGTGTGCATTGCCACCGTTACTGCAGTGAGAGGCTCGTACCTGTGGCTCCAGCTAGAAGGTTCTAAG; AAGCCTGTACCCGAATTTATTGTAAGTGCAGAGTCGATGAATATATTTCCTTTGGGCTGGTGTGAAACCA; ATGGCCATCCTCTCAGTACTCCTCGCCGGGCACGAGGGCATAAACTGAGGAAAATCGCAGTGGTT; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207437 representing BC066188; Red=Cloning site Green=Tags(s); ; MSGEQQLDADLGSGVEVEEFSWEDYLEETGSTTVPYASFKHVDIRLQNGFAPGMKLEVALKNDPETYWVA; TIITACEQLLLLRYEGYGEDRKADFWCDIRKAGLYPIGWCQQNKKTLEAPEGIRDKVSDWNAFLQQTLIG; ACGPPVSLLEGLRNGRNPLDLIAPGSKLECQDFRDSLSTWLVTVVENIGGRLKLRYEGLESRDGFEHWLY; YLDPFLHHIGWAAQQGCDLQPPLAIKHLKSEADWQEILAKVKEEEPLPSYLFKDKQVIGTHEFSINMKLE; AVDPWSPFGISPATIAKVFDDKYFLVEMDDLRPEDHTRRSFVCHANSPGIFPVQWSLKNGLHINPPPGFR; SQDFDWADYLKQCGAEAAPQKCFPQSISEHQFKENMKLEAVNPLFPEEVCIATVTAVRGSYLWLQLEGSK; KPVPEFIVSAESMNIFPLGWCETNGHPLSTPRRARGHKLRKIAVV; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC066188
• ORF Size: 1395 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC066188, AAH66188
• RefSeq Size: 2111 bp
• RefSeq ORF: 1397 bp
• Locus ID: 54650
• Cytogenetics: 14 18.99 cM
• Summary: Histone-binding protein, which is part of various corepressor complexes. Mediates the recruitment of corepressor complexes to target genes, followed by chromatin compaction and repression of transcription. Plays a role during myogenesis: required for the maintenance of undifferentiated states of myogenic progenitor cells via interaction with MYOD1. Interaction with MYOD1 leads to the recruitment of associated corepressors and silencing of MYOD1 target genes. Part of the SLC complex in germ cells, where it may play a role during spermatogenesis.[UniProtKB/Swiss-Prot Function]