Tulp3 (NM_011657) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG207340
Tulp3 (tGFP-tagged) - Mouse tubby-like protein 3 (Tulp3)
$489.00 $657.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tulp3
Synonyms 2310022L06Rik; AI316887
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG207340 representing NM_011657
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCGGCGCGCTGCGCACCCGGACCCCGCGGGGACAGTGCCTTTGACGATGAGACCCTGAGGCTTC
GGCAGCTGAAGCTGGACAATCAGAGGGCACTGCTGGAGAAGAAGCAGAGAAAGAAGCGCCTTGAGCCACT
CATGGTACAGCCAAACCCAGAAGCCAGGCTGCGAAGGTTAAAGCCACGAGGCAGTGAGGAGCACACGCCT
CTGGTGGACCCTCAGATGCCTCGCAGCGACGTCATCCTACACGGCATCGACGGTCCAGCTGCTTTCCTTA
AGCCAGAGGCTCAGGATTTGGAAAGCAAGCCTCAGGTTCTCTCTGTGGGCTCCCCTGCCCCAGAAGAAGG
CACGGAAGGGAGTGCAGATGGGGAAAGCCCCGAGGAGACAGCCCCCAAGCCAGACCTTCAGGAGATTCTC
CAAAAACACGGCATCTTGAGTAGTGTGAACTATGACGAGGAGCCTGACAAGGAGGAAGATGAGGGGGGAA
ACCTCAGCTCCCCGTCGGCTCGCTCAGAGGAGAGTGCTGCAGCCAGCCAGAAAGCAGCCTCGGAGACAGG
AGCTTCCGGTGTGACAGCCCAGCAGGGTGACGCCCAGCTTGGAGAAGTGGAGAATTTAGAGGACTTTGCG
TATAGCCCTGCCCCTCGGGGTGTCACGGTGAAGTGTAAAGTAACCAGGGATAAGAAAGGCATGGACCGTG
GCCTCTTCCCCACCTACTATATGCACCTGGAACGGGAGGAGAACCGCAAGATATTTCTTCTAGCCGGTAG
AAAGCGGAAAAAGAGCAAAACGTCCAACTACCTGGTCTCCACAGACCCGACAGATTTGTCTCGTGAAGGG
GAAAGTTACATTGGCAAACTCAGATCCAATCTCATGGGGACCAAGTTCACAGTGTATGACCATGGTGTCA
ACCCAGTCAAGGCCCAGGGTCTGGTGGAGAAGGCCCACACCCGCCAGGAGCTGGCTGCTATCTGTTATGA
AACAAATGTCCTCGGTTTCAAAGGTCCCAGGAAAATGTCTGTGATCATCCCGGGGATGAACATGAACCAT
GAGCGCATCCCTTTCAGGCCACGAAATGAACACGAGAGCTTGCTTTCAAAATGGCAAAACAAATCCATGG
AGAACCTGATCGAGCTGCACAACAAGGCCCCGGTGTGGAATGACGACACCCAGTCCTACGTGCTGAACTT
TCACGGCAGAGTCACCCAGGCATCCGTGAAAAACTTCCAGATCGTCCACGGGAATGACCCTGACTACATC
GTCATGCAGTTTGGACGCGTGGCTGATGACGTGTTCACACTGGACTATAACTACCCACTGTGCGCACTTC
AAGCCTTCGCCATCGGGCTGTCCAGCTTTGACAGCAAGCTGGCGTGTGAA


AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG207340 representing NM_011657
Red=Cloning site Green=Tags(s)

MEAARCAPGPRGDSAFDDETLRLRQLKLDNQRALLEKKQRKKRLEPLMVQPNPEARLRRLKPRGSEEHTP
LVDPQMPRSDVILHGIDGPAAFLKPEAQDLESKPQVLSVGSPAPEEGTEGSADGESPEETAPKPDLQEIL
QKHGILSSVNYDEEPDKEEDEGGNLSSPSARSEESAAASQKAASETGASGVTAQQGDAQLGEVENLEDFA
YSPAPRGVTVKCKVTRDKKGMDRGLFPTYYMHLEREENRKIFLLAGRKRKKSKTSNYLVSTDPTDLSREG
ESYIGKLRSNLMGTKFTVYDHGVNPVKAQGLVEKAHTRQELAAICYETNVLGFKGPRKMSVIIPGMNMNH
ERIPFRPRNEHESLLSKWQNKSMENLIELHNKAPVWNDDTQSYVLNFHGRVTQASVKNFQIVHGNDPDYI
VMQFGRVADDVFTLDYNYPLCALQAFAIGLSSFDSKLACE

SGPTRTRRLE - GFP Tag - V
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_011657
ORF Size 1380 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011657.2, NP_035787.1
RefSeq Size 3401 bp
RefSeq ORF 1383 bp
Locus ID 22158
UniProt ID O88413
Cytogenetics 6 62.96 cM
Summary Negative regulator of the Shh signaling transduction pathway: recruited to primary cilia via association with the IFT complex A (IFT-A) and is required for recruitment of G protein-coupled receptor GPR161 to cilia, a promoter of PKA-dependent basal repression machinery in Shh signaling. Binds to phosphorylated inositide (phosphoinositide) lipids. Both IFT-A- and phosphoinositide-binding properties are required to regulate ciliary G protein-coupled receptor trafficking. Not involved in ciliogenesis.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_011657" in other vectors (4)
SKU Description Size Price
MC202681 Tulp3 (untagged) - Mouse tubby-like protein 3 (Tulp3), (10ug) 10 ug
$457.00
MR207340 Tulp3 (Myc-DDK-tagged) - Mouse tubby-like protein 3 (Tulp3) 10 ug
$289.00 $457.00
MR207340L3 Lenti ORF clone of Tulp3 (Myc-DDK-tagged) - Mouse tubby-like protein 3 (Tulp3) 10 ug
$757.00
MR207340L4 Lenti ORF clone of Tulp3 (mGFP-tagged) - Mouse tubby-like protein 3 (Tulp3) 10 ug
$757.00

Plasmid Map for MG207340

Download sequence for

Cloning scheme for MG207340

Circular map for MG207340

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