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Tubd1 (NM_019756) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG207275
Tubd1 (tGFP-tagged) - Mouse tubulin, delta 1 (Tubd1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tubd1
Synonyms 4930550G19Rik; Tubd
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG207275 representing NM_019756
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGATAGTGACAGTACAGCTGGGGCAGTGTGGCAACCAGATTGGCTTTGAAGTGTTTGATGCTTTAT
TTAGAGACTCACACTGTTCCCAGGGACTCTGTTCTAAGAGGGACAACGAGGCATATCAAGCTTCCTGCAG
AGAAAGATTTTTCAGAGAGGAAGAAAATGGAGTTCCAGTTGCCCGGGCTGTTCTTGTTGACATGGAACCT
AAAGTTATCAATCAAACACTATCAAAAGCTGCCCAGTCTGGCCGATGGAATTATGGTCAGCACACGAGTT
TTTGTCAGAAACAAGGATCTGGGAACAACTGGGCATACGGTTACTCTGTGCATGGACCGAAGCATGAAGA
ATCTATAATGAACTTAATCCAAACAGAGGTGGAGAAATGTGACTCTTTGAGTGGATTTTTCATCATAATG
AGTATGGCCGGGGGAACAGGATCAGGGCTGGGAGCCTTCGTTACACAGAAGCTACAAGATCAGTACTCCA
GTTCCTTGAAAATGAACCAGATTATATGGCCTTATGGAACGGGTGAGGTCATTGTGCAGAACTACAACTC
CATCTTGACTCTCTCTCACCTGTACCGGTCTTCAGATGCCCTTCTCATTCACGAGAATGACGCTGTCCAT
AAGATCTGTGCCAAACGGATGAACATCAAGCAGATCTCCTTCAGAGACTTAAATCAAGTTCTTGCCCACC
AGCTGGGGAGTGTGTTCCAGCCTACATACTCTGAGGACAGCTCATTTCACTACCGCAGAAATCCACTAGG
AGACTTAATGGAGCATTTAGTTCCTCACCCTGAATTTAAGATGCTGGGTGTCCGTAACATTCCTCAGATG
TCTGCGGCGTCTCTGGCGTACAGCGCGTTTACCTGGGCTGGCCTCCTCAAGCACTTAAGACAGATGCTTA
TTTCCAGTGCAAAAATGGAAGAAGGTATCAACTGGCAGGTGCGGCCTCCTCTGACAGGCCTTCCACCCAT
TGGCAAAGCGTCTGCCCACAAGGAGCATCATTTTAACACTTCTCTTGCTAACTTGGTGGTCCTCCGAGGG
AGAGAGGTGCACAGTGCAGACGTAGAGGGATTTAAAGACCCAGCTCTCTACACTTCCTGGTTGGAGCCTG
TTGATGCTTTCAGTGTATGGAAAACCCAGCGAGCCTTTGACAAGTATGAGAAGTCTGCAGCCCTGGTCAG
CAACAGCCAGCTCTTAGTAAAGCCACTGGATATGATTGTGGGCAAGGCGTGGAATATGTTCTCATCCAAA
GCCTTTATCCATCAGTACACAAAATTTGGAATGGAGGAGGAAGACTTTTTGGACAGCTTTGCATTGTTGG
AGCAAGTTGTTGCCAGTTATGGTAGTCTTGGACCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG207275 representing NM_019756
Red=Cloning site Green=Tags(s)

MSIVTVQLGQCGNQIGFEVFDALFRDSHCSQGLCSKRDNEAYQASCRERFFREEENGVPVARAVLVDMEP
KVINQTLSKAAQSGRWNYGQHTSFCQKQGSGNNWAYGYSVHGPKHEESIMNLIQTEVEKCDSLSGFFIIM
SMAGGTGSGLGAFVTQKLQDQYSSSLKMNQIIWPYGTGEVIVQNYNSILTLSHLYRSSDALLIHENDAVH
KICAKRMNIKQISFRDLNQVLAHQLGSVFQPTYSEDSSFHYRRNPLGDLMEHLVPHPEFKMLGVRNIPQM
SAASLAYSAFTWAGLLKHLRQMLISSAKMEEGINWQVRPPLTGLPPIGKASAHKEHHFNTSLANLVVLRG
REVHSADVEGFKDPALYTSWLEPVDAFSVWKTQRAFDKYEKSAALVSNSQLLVKPLDMIVGKAWNMFSSK
AFIHQYTKFGMEEEDFLDSFALLEQVVASYGSLGP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019756
ORF Size 1365 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019756.3
RefSeq Size 1877 bp
RefSeq ORF 1368 bp
Locus ID 56427
UniProt ID Q9R1K7
Cytogenetics 11 C
Summary Acts as a positive regulator of hedgehog signaling and regulates ciliary function (PubMed:29290584).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_019756" in other vectors (3)
SKU Description Size Price
MR207275 Tubd1 (Myc-DDK-tagged) - Mouse tubulin, delta 1 (Tubd1), transcript variant 2 10 ug
$289.00 $457.00
MR207275L3 Lenti ORF clone of Tubd1 (Myc-DDK-tagged) - Mouse tubulin, delta 1 (Tubd1), transcript variant 2 10 ug
$757.00
MR207275L4 Lenti ORF clone of Tubd1 (mGFP-tagged) - Mouse tubulin, delta 1 (Tubd1), transcript variant 2 10 ug
$757.00

Plasmid Map for MG207275

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG207275
Circular map for MG207275

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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