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Tgfb1i1 (NM_009365) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG207081
Tgfb1i1 (tGFP-tagged) - Mouse transforming growth factor beta 1 induced transcript 1 (Tgfb1i1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tgfb1i1
Synonyms ARA55; hic-5; Hic5; TSC-5
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG207081 representing NM_009365
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCACGGTTAGGGGCTCCAAAAGAGCGCCCACCAGAGACACTCACACCTCCCCCACCCTATGGCCACC
AGCCACAGACAGGGTCTGGAGAATCTTCAGGAACCACTGGGGACAAGGATCATCTATACAGCACAGTATG
CAAGCCTCGGTCCCCAAAGCCTGTGGCCCCTGTGGCTCCTCCATTCTCCTCTTCCAGTGGTGTGTTGGGC
AATGGCCTCTGTGAGCTAGACCGTTTGCTTCAGGAACTTAATGCCACCCAGTTCAACATTACAGATGAAA
TCATGTCTCAGTTCCCATCTAGTAAAATGGCTGAAGGGGAAGAGAAGGAGGACCAATCTGAAGACAAGAG
CTCACCCACTGTCCCTCCCAGCCCATTCCCTGCCCCCTCAAAGCCTTCAGCCACCTCCGCCACTCAGGAA
CTGGATAGACTGATGGCCTCGCTCTCTGACTTCCGTGTTCAGAACCATCTTCCAGCCTCAGGGCCACCTC
AGCCTCCAGCAGCGAGCCCCACCCGTGAAGGATGCCCATCTCCACCAGGACAGACTAGCAAAGGCAGTCT
GGACACCATGCTGGGCCTGCTGCAGTCTGACCTCAGCCGTCGTGGTGTCCCCACACAGGCCAAAGGCCTC
TGTGGCTCCTGCAATAAACCTATAGCTGGGCAAGTGGTTACAGCCCTGGGCAGAGCCTGGCACCCGGAGC
ACTTCCTTTGCAGCGGTTGTTCCACAACCCTGGGAGGCAGCAGCTTCTTCGAGAAGGATGGGGCTCCCTT
TTGCCCCGAGTGCTACTTTGAGCGCTTCTCCCCACGATGTGGCTTCTGTAACCAACCCATCCGACACAAA
ATGGTTACCGCCTTGGGCACCCACTGGCACCCAGAACATTTCTGCTGCGTCAGCTGCGGAGAGCCTTTTG
GAGAAGAGGGTTTCCACGAGCGGGAGGGTCGTCCGTACTGCCGGCGGGACTTCCTGCAGCTGTTCGCCCC
ACGCTGCCAGGGCTGCCAAGGCCCTATTTTGGATAACTACATCTCGGCACTCAGCGCGCTCTGGCACCCA
GACTGCTTCGTGTGCAGGGAATGCCTTGCGCCCTTCTCCGGAGGCAGCTTTTTTGAGCACGAGGGTCGCC
CTTTGTGTGAAAACCATTTCCATGCTCAGCGTGGTTCGCTGTGTGCCACGTGTGGTCTCCCAGTGACCGG
CCGCTGTGTGTCCGCTCTGGGCCGACGCTTCCATCCAGACCACTTCACCTGCACATTCTGCCTGCGCCCA
CTCACCAAAGGCTCCTTCCAGGAGCGCGCCAGCAAGCCTTACTGCCAGCCTTGCTTCCTGAAGCTCTTCG
GC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG207081 representing NM_009365
Red=Cloning site Green=Tags(s)

MSRLGAPKERPPETLTPPPPYGHQPQTGSGESSGTTGDKDHLYSTVCKPRSPKPVAPVAPPFSSSSGVLG
NGLCELDRLLQELNATQFNITDEIMSQFPSSKMAEGEEKEDQSEDKSSPTVPPSPFPAPSKPSATSATQE
LDRLMASLSDFRVQNHLPASGPPQPPAASPTREGCPSPPGQTSKGSLDTMLGLLQSDLSRRGVPTQAKGL
CGSCNKPIAGQVVTALGRAWHPEHFLCSGCSTTLGGSSFFEKDGAPFCPECYFERFSPRCGFCNQPIRHK
MVTALGTHWHPEHFCCVSCGEPFGEEGFHEREGRPYCRRDFLQLFAPRCQGCQGPILDNYISALSALWHP
DCFVCRECLAPFSGGSFFEHEGRPLCENHFHAQRGSLCATCGLPVTGRCVSALGRRFHPDHFTCTFCLRP
LTKGSFQERASKPYCQPCFLKLFG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009365
ORF Size 1332 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009365.2, NP_033391.1
RefSeq Size 3058 bp
RefSeq ORF 1335 bp
Locus ID 21804
Cytogenetics 7 F3
Summary Functions as a molecular adapter coordinating multiple protein-protein interactions at the focal adhesion complex and in the nucleus. Links various intracellular signaling modules to plasma membrane receptors and regulates the Wnt and TGFB signaling pathways. May also regulate SLC6A3 and SLC6A4 targeting to the plasma membrane hence regulating their activity. In the nucleus, functions as a nuclear receptor coactivator regulating glucocorticoid, androgen, mineralocorticoid and progesterone receptor transcriptional activity. May play a role in the processes of cell growth, proliferation, migration, differentiation and senescence. May have a zinc-dependent DNA-binding activity.UniProtKB/Swiss-Prot Function
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Resources
"NM_009365" in other vectors (4)
SKU Description Size Price
MC202670 Tgfb1i1 (untagged) - Mouse transforming growth factor beta 1 induced transcript 1 (Tgfb1i1), (10ug) 10 ug
$457.00
MR207081 Tgfb1i1 (Myc-DDK-tagged) - Mouse transforming growth factor beta 1 induced transcript 1 (Tgfb1i1) 10 ug
$289.00 $457.00
MR207081L3 Lenti ORF clone of Tgfb1i1 (Myc-DDK-tagged) - Mouse transforming growth factor beta 1 induced transcript 1 (Tgfb1i1) 10 ug
$757.00
MR207081L4 Lenti ORF clone of Tgfb1i1 (mGFP-tagged) - Mouse transforming growth factor beta 1 induced transcript 1 (Tgfb1i1) 10 ug
$757.00

Plasmid Map for MG207081

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG207081
Circular map for MG207081

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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