Cmas (NM_009908) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG206899
Cmas (tGFP-tagged) - Mouse cytidine monophospho-N-acetylneuraminic acid synthetase (Cmas). Note: ORF is codon optimized
  $703.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cmas
Synonyms AW208911; CMPNeu5Ac; D6Bwg0250e
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG206899 representing NM_009908, codon optimized.
Due to the complexity of NM_009908, the ORF clone is codon optimized for mammalian Expression.
The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical
.

Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGCCCTTGAAAAGGGTGCAGCAACATCCGGCCCTGCTCCAAGGGGACGGCCTTCTCGCGGCCGGC
CACCAAAGCTTCAAAGGTCAAGGGGCGCGGGAAGAGGTCTCGAGAAACCACCCCACTTGGCAGCACTTGT
GCTGGCCCGGGGGGGAAGCAAGGGGATCCCTCTGAAGAACATAAAGAGGTTGGCTGGGGTGCCTCTGATC
GGCTGGGTCCTTCGGGCTGCGCTGGATGCTGGAGTTTTCCAGTCCGTGTGGGTATCTACAGACCATGACG
AAATCGAAAACGTAGCCAAACAGTTTGGAGCCCAGGTGCACAGGAGAAGTTCAGAAACAAGTAAGGATTC
CAGTACAAGCCTGGACGCCATCGTGGAATTTCTGAATTACCACAATGAAGTGGATATCGTTGGCAACATC
CAGGCCACGAGCCCCTGCCTGCACCCAACAGATCTGCAGAAGGTGGCCGAGATGATCAGGGAGGAAGGGT
ATGATAGTGTTTTCTCTGTTGTCCGGAGGCATCAGTTCCGGTGGTCCGAGATTCAAAAGGGGGTGCGGGA
AGTGACAGAACCACTCAACCTCAACCCTGCCAAGCGCCCTCGCCGACAGGATTGGGATGGAGAACTGTAT
GAAAACGGGTCTTTTTATTTCGCCAAACGCCACCTGATAGAAATGGGGTACCTGCAGGGAGGCAAGATGG
CATATTACGAGATGAGAGCAGAACACTCCGTGGATATCGACGTGGATATCGACTGGCCCATCGCCGAGCA
GAGGGTTCTGCGATTTGGCTACTTCGGTAAGGAAAAGTTGAAGGAGATCAAGCTCCTGGTGTGTAACATC
GACGGCTGCTTGACCAACGGACATATCTATGTGTCCGGTGACCAGAAAGAGATCATCTCCTACGATGTGA
AAGATGCTATCGGTATTAGTCTGTTGAAAAAATCTGGCATCGAGGTTCGATTGATTTCTGAGAGAGCTTG
CAGTAAGCAGACTCTCTCTGCACTGAAACTCGATTGCAAAACGGAAGTTTCCGTGTCTGACAAGCTGGCA
ACTGTGGATGAGTGGCGCAAAGAGATGGGCCTGTGTTGGAAGGAAGTGGCTTATCTGGGGAATGAAGTTT
CCGATGAAGAGTGCCTGAAGCGCGTGGGACTGTCCGCAGTACCAGCTGATGCATGTAGTGGCGCCCAGAA
GGCCGTCGGCTATATCTGCAAGTGTAGCGGTGGAAGAGGCGCCATCCGCGAATTCGCAGAGCATATTTTT
CTCCTGATTGAGAAGGTCAATAATTCCTGCCAAAAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG206899 representing NM_009908
Red=Cloning site Green=Tags(s)

MDALEKGAATSGPAPRGRPSRGRPPKLQRSRGAGRGLEKPPHLAALVLARGGSKGIPLKNIKRLAGVPLI
GWVLRAALDAGVFQSVWVSTDHDEIENVAKQFGAQVHRRSSETSKDSSTSLDAIVEFLNYHNEVDIVGNI
QATSPCLHPTDLQKVAEMIREEGYDSVFSVVRRHQFRWSEIQKGVREVTEPLNLNPAKRPRRQDWDGELY
ENGSFYFAKRHLIEMGYLQGGKMAYYEMRAEHSVDIDVDIDWPIAEQRVLRFGYFGKEKLKEIKLLVCNI
DGCLTNGHIYVSGDQKEIISYDVKDAIGISLLKKSGIEVRLISERACSKQTLSALKLDCKTEVSVSDKLA
TVDEWRKEMGLCWKEVAYLGNEVSDEECLKRVGLSAVPADACSGAQKAVGYICKCSGGRGAIREFAEHIF
LLIEKVNNSCQK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009908
ORF Size 1296 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009908.2, NP_034038.2
RefSeq Size 1760 bp
RefSeq ORF 1299 bp
Locus ID 12764
UniProt ID Q99KK2
Cytogenetics 6 74.66 cM
Summary Catalyzes the activation of N-acetylneuraminic acid (NeuNAc) to cytidine 5'-monophosphate N-acetylneuraminic acid (CMP-NeuNAc), a substrate required for the addition of sialic acid. Has some activity toward NeuNAc, N-glycolylneuraminic acid (Neu5Gc) or 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_009908" in other vectors (4)
SKU Description Size Price
MC201490 Cmas (untagged) - Mouse cytidine monophospho-N-acetylneuraminic acid synthetase (Cmas), (10ug) 10 ug
$457.00
MR206899 Cmas (Myc-DDK-tagged) - Mouse cytidine monophospho-N-acetylneuraminic acid synthetase (Cmas). Note: ORF is codon optimized 10 ug
$503.00
MR206899L3 Lenti ORF clone of Cmas (Myc-DDK-tagged) - Mouse cytidine monophospho-N-acetylneuraminic acid synthetase (Cmas). Note: ORF is codon optimized 10 ug
$803.00
MR206899L4 Lenti ORF clone of Cmas (mGFP-tagged) - Mouse cytidine monophospho-N-acetylneuraminic acid synthetase (Cmas). Note: ORF is codon optimized 10 ug
$803.00

Plasmid Map for MG206899

Download sequence for

Cloning scheme for MG206899

Circular map for MG206899

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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