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Rnf128 (NM_023270) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG206827
Rnf128 (tGFP-tagged) - Mouse ring finger protein 128 (Rnf128)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
$489.00 $657.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rnf128
Synonyms 1300002C13Rik; AI987883; GRAIL; Greul1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG206827 representing NM_023270
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCCGCCGCCCGGGATCGGGGTCTACTGCCGCGGCGGCTGCGGAGCTGCCCGGCTACTGGCTTGGT
GCTTCCTTCTGGCTCTGAGTCCGCACGCGCCCGGTTCCCGGGGAGCCGAAGCCGTGTGGACCGCGTACCT
CAACGTGTCCTGGCGGGTTCCGCACACCGGAGTGAACCGCACGGTGTGGGAGCTGAGCGAGGAGGGCGTG
TACGGCCAGGACTCGCCGCTGGAGCCCGTCTCCGGGGTCCTGGTACCGCCCGACGGGCCCGGGGCGCTCA
ACGCCTGTAACCCGCACACCAATTTCACGGTGCCCACGGTTTGGGGGAGCACGGTGCAAGTATCTTGGTT
GGCCCTCATCCAACGCGGTGGAGGCTGCACCTTCGCGGACAAGATCCATCTGGCTTCAGAGAGAGGGGCT
TCTGGAGCGGTCATCTTTAACTTCCCTGGGACCCGCAATGAGGTCATCCCCATGTCTCACCCGGGTGCTG
GGGACATTGTTGCAATCATGATTGGCAATCTGAAAGGAACAAAAATTCTGCAGTCTATTCAAAGAGGCAT
CCAAGTCACAATGGTCATCGAAGTAGGGAAAAAACATGGCCCTTGGGTGAATCATTATTCAATTTTCTTC
GTTTCTGTGTCCTTTTTCATAATTACGGCAGCAACCGTGGGCTATTTCATCTTTTATTCTGCTCGAAGAT
TACGAAATGCAAGAGCTCAAAGCAGGAAGCAGAGGCAGTTAAAGGCAGATGCTAAAAAAGCTATTGGAAA
GCTTCAGCTGCGCACCTTGAAACAAGGAGACAAGGAAATTGGCCCTGATGGAGATAGCTGTGCTGTGTGC
ATTGAGCTCTATAAGCCAAATGATTTGGTGCGCATCCTAACCTGCAATCATATTTTCCATAAGACATGTG
TGGACCCGTGGCTTTTAGAACACAGGACTTGCCCCATGTGCAAGTGTGACATTCTCAAAGCTCTGGGAAT
TGAGGTGGATGTTGAAGATGGATCAGTGTCTTTACAAGTTCCTGTTTCTAATGAAGCATCTAATACTGCC
TCTCCCCATGAAGAGGACAGTCGCAGTGAGACTGCATCATCTGGATATGCTTCAGTACAAGGAGCAGATG
AGCCACCTCTGGAGGAACATGCGCAGTCAGCAAATGAAAATCTACAGCTGGTAAACCATGAAGCAAATTC
TGTGGCCGTGGATGTTGTTCCCCATGTTGACAACCCAACCTTTGAAGAAGATGAAACTCCTGATCAAGAG
GCAGCTGTTCGGGAGATTAAATCT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG206827 representing NM_023270
Red=Cloning site Green=Tags(s)

MGPPPGIGVYCRGGCGAARLLAWCFLLALSPHAPGSRGAEAVWTAYLNVSWRVPHTGVNRTVWELSEEGV
YGQDSPLEPVSGVLVPPDGPGALNACNPHTNFTVPTVWGSTVQVSWLALIQRGGGCTFADKIHLASERGA
SGAVIFNFPGTRNEVIPMSHPGAGDIVAIMIGNLKGTKILQSIQRGIQVTMVIEVGKKHGPWVNHYSIFF
VSVSFFIITAATVGYFIFYSARRLRNARAQSRKQRQLKADAKKAIGKLQLRTLKQGDKEIGPDGDSCAVC
IELYKPNDLVRILTCNHIFHKTCVDPWLLEHRTCPMCKCDILKALGIEVDVEDGSVSLQVPVSNEASNTA
SPHEEDSRSETASSGYASVQGADEPPLEEHAQSANENLQLVNHEANSVAVDVVPHVDNPTFEEDETPDQE
AAVREIKS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_023270
ORF Size 1284 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_023270.5, NP_075759.3
RefSeq Size 2764 bp
RefSeq ORF 1287 bp
Locus ID 66889
UniProt ID Q9D304
Cytogenetics X F1
Summary E3 ubiquitin-protein ligase that catalyzes 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains formation. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription, thereby playing an important role in the induction of the anergic phenotype, a long-term stable state of T-lymphocyte unresponsiveness to antigenic stimulation associated with the blockade of interleukin production. Ubiquitinates ARPC5 with 'Lys-48' linkages and COR1A with 'Lys-63' linkages leading to their degradation, down-regulation of these cytosleletal components results in impaired lamellipodium formation and reduced accumulation of F-actin at the immunological synapse. Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural-inducing signals (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_023270" in other vectors (4)
SKU Description Size Price
MC201428 Rnf128 (untagged) - Mouse ring finger protein 128 (Rnf128), (10ug) 10 ug
$457.00
MR206827 Rnf128 (Myc-DDK-tagged) - Mouse ring finger protein 128 (Rnf128) 10 ug
$289.00 $457.00
MR206827L3 Lenti ORF clone of Rnf128 (Myc-DDK-tagged) - Mouse ring finger protein 128 (Rnf128) 10 ug
$757.00
MR206827L4 Lenti ORF clone of Rnf128 (mGFP-tagged) - Mouse ring finger protein 128 (Rnf128) 10 ug
$757.00

Plasmid Map for MG206827

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG206827
Circular map for MG206827

file-alt Citations

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