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Pacsin3 (NM_030880) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG206762
Pacsin3 (tGFP-tagged) - Mouse protein kinase C and casein kinase substrate in neurons 3 (Pacsin3)
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  $657.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pacsin3
Synonyms 4921507A02Rik; 6330413E15Rik; AW413130
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG206762 representing NM_030880
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCCAGAGGAGGACGCCGGAGGGGAGGTCCTAGGGGGCAGTTTCTGGGAGGCTGGCAACTACAGAC
GGACGGTGCAGAGGGTTGAGGATGGGCACAGGCTGTGTGGGGACCTGGTTAGCTGCTTCCAAGAACGTGC
CCGCATTGAGAAGGCCTACGCCCAGCAGCTGGCTGACTGGGCCCGCAAGTGGAGGGGTGCTGTGGAGAAG
GGCCCACAGTACGGCACCCTGGAGAAGGCCTGGCATGCCTTCTTCACTGCGGCTGAGAGGCTGAGCGAGC
TGCACTTGGAGGTGAGGGAGAAGTTGCACGGTCCAGACAGCGAGCGAGTGCGGACCTGGCAGCGAGGGGC
TTTCCACCGGCCAGTGCTGGGGGGCTTTCGGGAAAGCCGGGCTGCAGAGGATGGTTTCCGTAAAGCTCAG
AAGCCCTGGCTAAAGAGGCTGAAGGAGGTTGAGGCTTCTAAGAAGAGCTACCACACAGCCCGCAAGGATG
AGAAGACAGCCCAGACCCGGGAGAGCCACGCGAAGGCAGACAGCTCCATGTCCCAGGAGCAGCTTCGAAA
ATTACAGGAGCGGGTGGGCCGCTGCACCAAGGAGGCAGAGAAGATGAAAACCCAGTATGAGCAGACCCTG
GCCGAGCTAAATCGCTACACCCCACGCTACATGGAGGACATGGAGCAGGCTTTTGAGAGCTGCCAGGCTG
CTGAGCGCCAGCGGCTTCTCTTCTTCAAGGATGTCTTGCTCACCCTGCATCAACACCTCGACCTCTCCAG
TAGCGACAAGTTCCATGAACTCCATCGAGACCTGCAGCAGAGCATTGAGGCTGCCAGTGATGAAGAGGAT
CTGCGCTGGTGGCGGAGCACACACGGGCCTGGCATGGCCATGAACTGGCCACAGTTTGAGGAATGGTCGT
TAGACACACAGAGAGCCATCAGCCGCAAGGAGAAGGGTGGCCGGAGCCCAGATGAGGTTACTCTGACCAG
CATTGTACCGACAAGAGATGGCACCGCACCCCCACCCCAGTCCCCATCGTCCCCGGGTAGCGGGCAGGAC
GAGGACTGGTCAGATGAGGAGAGTCCCCGGAAAGTTGCCACTGGGGTGAGGGTACGGGCGCTTTATGACT
ATGCTGGCCAGGAGGCTGATGAGCTGAGCTTCCGAGCAGGGGAAGAGTTGCTGAAGATGAGCGAGGAGGA
TGAGCAGGGCTGGTGCCAGGGCCAGCTGCAGAGTGGCCGCATTGGTCTGTACCCTGCCAATTACGTAGAA
TGTGTGGGCGCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG206762 representing NM_030880
Red=Cloning site Green=Tags(s)

MAPEEDAGGEVLGGSFWEAGNYRRTVQRVEDGHRLCGDLVSCFQERARIEKAYAQQLADWARKWRGAVEK
GPQYGTLEKAWHAFFTAAERLSELHLEVREKLHGPDSERVRTWQRGAFHRPVLGGFRESRAAEDGFRKAQ
KPWLKRLKEVEASKKSYHTARKDEKTAQTRESHAKADSSMSQEQLRKLQERVGRCTKEAEKMKTQYEQTL
AELNRYTPRYMEDMEQAFESCQAAERQRLLFFKDVLLTLHQHLDLSSSDKFHELHRDLQQSIEAASDEED
LRWWRSTHGPGMAMNWPQFEEWSLDTQRAISRKEKGGRSPDEVTLTSIVPTRDGTAPPPQSPSSPGSGQD
EDWSDEESPRKVATGVRVRALYDYAGQEADELSFRAGEELLKMSEEDEQGWCQGQLQSGRIGLYPANYVE
CVGA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_030880
ORF Size 1272 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030880.3
RefSeq Size 1767 bp
RefSeq ORF 1275 bp
Locus ID 80708
UniProt ID Q99JB8
Cytogenetics 2 E1
Summary Plays a role in endocytosis and regulates internalization of plasma membrane proteins. Overexpression impairs internalization of SLC2A1/GLUT1 and TRPV4 and increases the levels of SLC2A1/GLUT1 and TRPV4 at the cell membrane. Inhibits the TRPV4 calcium channel activity.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_030880" in other vectors (1)
SKU Description Size Price
MC200174 Pacsin3 (untagged) - Mouse protein kinase C and casein kinase substrate in neurons 3 (Pacsin3), (10ug) 10 ug
$457.00

Plasmid Map for MG206762

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG206762
Circular map for MG206762

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