Eif4a3 (NM_138669) Mouse Tagged ORF Clone

SKU MG206460

Eif4a3 (tGFP-tagged) - Mouse eukaryotic translation initiation factor 4A, isoform 3 (Eif4a3)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Eif4a3
• Synonyms: 2400003O03Rik; Ddx48; eIF4A-III; mKIAA0111
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG206460 representing NM_138669; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGGCTAACGCCACGATGGCGACGTCTGGCTCGGCGCGGAAGCGGCTGCTCAAAGAGGAGGACATGA; CCAAAGTGGAGTTCGAGACGAGCGAGGAGGTGGACGTGACCCCCACGTTCGACACCATGGGCCTGCGAGA; GGACCTGCTGCGCGGCATCTACGCCTACGGTTTTGAAAAACCTTCAGCGATTCAGCAGCGTGCTATCAAG; CAGATAATTAAAGGGAGAGATGTCATTGCACAGTCTCAGTCTGGCACAGGCAAGACGGCCACCTTCAGTG; TCTCAGTGCTGCAGTGCTTGGATATCCAGGTTCGAGAAACCCAAGCTTTGATCCTGGCTCCAACCAGGGA; GTTAGCGGTGCAGATTCAGAAGGGTCTGCTCGCGCTGGGGGATTACATGAACGTGCAGTGCCATGCCTGC; ATTGGGGGCACCAACGTCGGCGAGGACATCCGGAAGCTGGACTACGGACAGCACGTGGTGGCAGGCACGC; CGGGACGCGTCTTTGATATGATCCGCCGGAGAAGTTTACGGACACGGGCTATCAAGATGCTGGTTTTGGA; TGAGGCTGATGAAATGTTGAACAAAGGTTTCAAGGAGCAGATCTATGATGTGTACAGGTACTTGCCACCA; GCCACACAGGTCGTCCTCATCAGCGCCACACTGCCTCATGAGATCCTGGAGATGACCAACAAGTTCATGA; CCGACCCCATCCGCATCTTGGTGAAGCGTGATGAGTTGACTCTGGAAGGCATCAAACAGTTCTTTGTGGC; TGTGGAAAGAGAGGAATGGAAATTTGATACTCTATGTGATCTCTATGACACGCTGACCATCACCCAGGCC; GTCATCTTCTGCAACACCAAGCGGAAGGTTGACTGGCTGACAGAGAAAATGAGAGAAGCCAATTTCACTG; TGTCGTCCATGCATGGAGACATGCCCCAGAAAGAACGAGAGTCTATCATGAAGGAGTTCCGGTCAGGTGC; CAGCCGGGTGCTCATCTCCACAGACGTCTGGGCCCGGGGCCTGGATGTCCCTCAGGTGTCCCTCATCATT; AACTACGACCTGCCCAACAACAGAGAACTGTACATTCACAGAATTGGGAGATCGGGTCGGTATGGACGAA; AAGGTGTGGCCATCAATTTTGTGAAGAATGATGACATCCGGATTCTCAGGGACATTGAGCAGTACTACTC; CACCCAGATAGACGAGATGCCCATGAATGTGGCTGACCTCATC; ; AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG206460 representing NM_138669; Red=Cloning site Green=Tags(s); ; MAANATMATSGSARKRLLKEEDMTKVEFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQRAIK; QIIKGRDVIAQSQSGTGKTATFSVSVLQCLDIQVRETQALILAPTRELAVQIQKGLLALGDYMNVQCHAC; IGGTNVGEDIRKLDYGQHVVAGTPGRVFDMIRRRSLRTRAIKMLVLDEADEMLNKGFKEQIYDVYRYLPP; ATQVVLISATLPHEILEMTNKFMTDPIRILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQA; VIFCNTKRKVDWLTEKMREANFTVSSMHGDMPQKERESIMKEFRSGASRVLISTDVWARGLDVPQVSLII; NYDLPNNRELYIHRIGRSGRYGRKGVAINFVKNDDIRILRDIEQYYSTQIDEMPMNVADLI; ; SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138669
• ORF Size: 1233 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_138669.1, NP_619610.1
• RefSeq Size: 1489 bp
• RefSeq ORF: 1236 bp
• Locus ID: 192170
• UniProt ID: Q91VC3
• Cytogenetics: 11 E2
• Summary: ATP-dependent RNA helicase. Involved in pre-mRNA splicing as component of the spliceosome. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH-RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms; the function is different from the established EJC assembly. Involved in craniofacial development.[UniProtKB/Swiss-Prot Function]