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Mvd (NM_138656) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG206298
Mvd (tGFP-tagged) - Mouse mevalonate (diphospho) decarboxylase (Mvd)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mvd
Synonyms C78718; Mpd
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG206298 representing NM_138656
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTCAGAAAAGCCTCAGGACCTAATGGTCACCTGCACGGCGCCGGTCAACATCGCAGTTATCAAGT
ACTGGGGGAAGCGAGATGAAGCGCTGATCCTGCCCATCAACTCCTCCCTGAGCGTCACGCTGCACCAGGA
CCAGCTAAAAACGACCACAACCGTTGCCATTAGCAAGGACTTCACAGAGGACCGCATCTGGCTGAACGGT
CGCGAGGAGGACGTGGGGCAGCCACGGCTCCAGGCCTGCCTGAGAGAGATTCGCCGCCTGGCTCGGAAGC
GGAGGAGCACAGAGGACGGGGACACTCTACCCCTCAGCCTCAGCTATAAGGTGCACGTGGCGTCAGTGAA
CAACTTCCCCACTGCTGCAGGCCTGGCGTCCTCAGCCGCAGGCTATGCCTGCCTAGCCTATACCCTGGCT
CAGGTCTATGGGGTTGAGGGAGACCTCTCCGAAGTGGCTCGGCGGGGCTCAGGCAGTGCGTGCCGCAGTC
TCTATGGGGGCTTTGTGGAGTGGCAGATGGGGGAACAGGCCGATGGGAAGGACAGCATCGCCCGGCAGAT
AGCCCCAGAGTGGCACTGGCCCCAGCTCCGAATCCTTATCCTTGTGGTGAGCGCCGACAAGAAGCAGACG
GGCAGTACAGTGGGCATGCAGACCAGCGTGGAGACCAGCACCCTGCTCAAGTTCCGGGCTGAGTCTGTTG
TGCCTGAGCGCATGAAGGAGATGACCCGCTGCATCCAAGAGCAGGACTTTCAGGGCTTTGCCCAGCTGAC
CATGAAGGATAGCAACCAGTTTCATGCCACCTGCCTGGACACCTTCCCACCGATCTCCTACCTCAATGAC
ACCTCCAGGCGCATCATCCAGCTAGTCCACCGCTTCAACACCCACCACGGGCAGACGAAGGTGGCATACA
CATTTGATGCTGGCCCCAACGCTGTGATCTTCACTCTGGAGGACACTGTGGCTGAGTTTGTGGCAGCTGT
AAGACACAGCTTTCCCCCTGCAGCCAATGGAGACAAGTTCCTAAAGGGGCTGCAGGTGGCACCTGTTCTT
CTCTCTGATGAGCTGAAGGCCGCGCTGGTGGTGGAGCCCAGCCCTGGTGGGGTCCAGTACATCATTGCCA
CTCAGGTCGGACCAGGACCTCAAGTCCTAGACGACACTCATGATCATCTGCTAGGCCAGGATGGACTGCC
ACAACGGGACCTT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG206298 representing NM_138656
Red=Cloning site Green=Tags(s)

MASEKPQDLMVTCTAPVNIAVIKYWGKRDEALILPINSSLSVTLHQDQLKTTTTVAISKDFTEDRIWLNG
REEDVGQPRLQACLREIRRLARKRRSTEDGDTLPLSLSYKVHVASVNNFPTAAGLASSAAGYACLAYTLA
QVYGVEGDLSEVARRGSGSACRSLYGGFVEWQMGEQADGKDSIARQIAPEWHWPQLRILILVVSADKKQT
GSTVGMQTSVETSTLLKFRAESVVPERMKEMTRCIQEQDFQGFAQLTMKDSNQFHATCLDTFPPISYLND
TSRRIIQLVHRFNTHHGQTKVAYTFDAGPNAVIFTLEDTVAEFVAAVRHSFPPAANGDKFLKGLQVAPVL
LSDELKAALVVEPSPGGVQYIIATQVGPGPQVLDDTHDHLLGQDGLPQRDL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_138656
ORF Size 1203 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_138656.1, NP_619597.1
RefSeq Size 1767 bp
RefSeq ORF 1206 bp
Locus ID 192156
UniProt ID Q99JF5
Cytogenetics 8 E1
Summary Performs the first committed step in the biosynthesis of isoprenes.UniProtKB/Swiss-Prot Function
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"NM_138656" in other vectors (4)
SKU Description Size Price
MC203671 Mvd (untagged) - Mouse mevalonate (diphospho) decarboxylase (Mvd), transcript variant 1, (10ug) 10 ug
$457.00
MR206298 Mvd (Myc-DDK-tagged) - Mouse mevalonate (diphospho) decarboxylase (Mvd), transcript variant 1 10 ug
$289.00 $457.00
MR206298L3 Lenti ORF clone of Mvd (Myc-DDK-tagged) - Mouse mevalonate (diphospho) decarboxylase (Mvd), transcript variant 1 10 ug
$757.00
MR206298L4 Lenti ORF clone of Mvd (mGFP-tagged) - Mouse mevalonate (diphospho) decarboxylase (Mvd), transcript variant 1 10 ug
$757.00

Plasmid Map for MG206298

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG206298
Circular map for MG206298

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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