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Lipf (NM_026334) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG206176
Lipf (tGFP-tagged) - Mouse lipase, gastric (Lipf)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
$489.00 $657.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Lipf
Synonyms 2310051B21Rik; AV082900
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG206176 representing NM_026334
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGCTGCTATTAGTAACAAGTGTGCTATCTGCATTTGGAGGTGCACATGGCCTATTTGGAAAACTGG
GTCCCAAAAACCCTGAAGCAAACATGAATGTTAGTCAGATGATAACTTACTGGGGATATCCAAGTGAGGA
ATATGAAGTTGTTACTGAAGATGGCTACATTCTGGGGGTCTATAGAATTCCTTATGGGAAGAAAAATTCT
GAGAATATCGGCAAGAGACCTGTGGCATATTTGCAGCATGGTTTGATTGCATCAGCCACAAACTGGATTA
CAAATCTGCCAAACAACAGCCTGGCCTTCATTCTAGCAGATGCTGGCTATGATGTGTGGCTGGGGAACAG
TCGAGGGAATACATGGTCCCGGAAAAATGTATACTATTCACCAGACTCAGTTGAATTCTGGGCTTTCAGC
TTTGATGAAATGGCTAAATATGACCTTCCAGCCACCATAGACTTCATTGTACAGAAAACTGGACAAGAGA
AGATACACTATGTTGGTCACTCTCAGGGCACCACTATCGGTTTTATTGCCTTTTCTACCAATCCTGCTCT
GGCTAAAAAAATCAAGAGGTTTTATGCATTAGCTCCAGTTGCTACTGTGAAGTATACAGAAAGTCCCTTT
AAAAAGATTTCACTTATTCCTAAGTTTCTTCTCAAGGTGATATTTGGTAACAAAATGTTCATGCCCCACA
ACTACTTAGATCAATTTCTTGGTACGGAAGTGTGCTCACGGGAGCTGCTAGATCTTCTCTGCAGCAACGC
TTTATTCATCTTCTGTGGATTTGACAAGAAAAACTTAAATGTGAGTCGCTTTGATGTGTATCTAGGGCAT
AATCCAGCAGGAACATCTACTCAAGACCTTTTCCACTGGGCACAGCTTGCTAAATCTGGGAAGCTTCAAG
CCTATAACTGGGGAAGTCCATTACAGAACATGTTACACTACAATCAGAAAACGCCTCCCTACTATGATGT
GTCAGCCATGACCGTGCCAATTGCAGTGTGGAACGGTGGCCATGACATCCTGGCTGATCCCCAAGATGTC
GCAATGCTGCTTCCCAAACTCCCCAACCTTCTGTACCATAAGGAGATTCTTCCCTACAATCACCTGGACT
TCATCTGGGCGATGGATGCGCCTCAAGAGGTTTACAATGAGATAGTTACCATGATGGCAGAAGAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG206176 representing NM_026334
Red=Cloning site Green=Tags(s)

MWLLLVTSVLSAFGGAHGLFGKLGPKNPEANMNVSQMITYWGYPSEEYEVVTEDGYILGVYRIPYGKKNS
ENIGKRPVAYLQHGLIASATNWITNLPNNSLAFILADAGYDVWLGNSRGNTWSRKNVYYSPDSVEFWAFS
FDEMAKYDLPATIDFIVQKTGQEKIHYVGHSQGTTIGFIAFSTNPALAKKIKRFYALAPVATVKYTESPF
KKISLIPKFLLKVIFGNKMFMPHNYLDQFLGTEVCSRELLDLLCSNALFIFCGFDKKNLNVSRFDVYLGH
NPAGTSTQDLFHWAQLAKSGKLQAYNWGSPLQNMLHYNQKTPPYYDVSAMTVPIAVWNGGHDILADPQDV
AMLLPKLPNLLYHKEILPYNHLDFIWAMDAPQEVYNEIVTMMAED

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026334
ORF Size 1185 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026334.3, NP_080610.1
RefSeq Size 1353 bp
RefSeq ORF 1188 bp
Locus ID 67717
UniProt ID Q9CPP7
Cytogenetics 19 C1
Summary Catalyzes the hydrolysis of triacylglycerols to yield free fatty acids, diacylglycerol, monoacylglycerol, and glycerol (By similarity). Shows a preferential hydrolysis at the sn-3 position of triacylglycerol (By similarity).UniProtKB/Swiss-Prot Function
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"NM_026334" in other vectors (4)
SKU Description Size Price
MC210610 Lipf (untagged) - Mouse lipase, gastric (Lipf), (10ug) 10 ug
$503.00
MR206176 Lipf (Myc-DDK-tagged) - Mouse lipase, gastric (Lipf) 10 ug
$289.00 $457.00
MR206176L3 Lenti ORF clone of Lipf (Myc-DDK-tagged) - Mouse lipase, gastric (Lipf) 10 ug
$757.00
MR206176L4 Lenti ORF clone of Lipf (mGFP-tagged) - Mouse lipase, gastric (Lipf) 10 ug
$757.00

Plasmid Map for MG206176

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG206176
Circular map for MG206176

file-alt Citations

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