Myohd1 (BC007156) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG206167
Myohd1 (tGFP-tagged) - Mouse myosin head domain containing 1 (cDNA clone MGC:7331 IMAGE:3486558)
  $657.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Myohd1
Synonyms 1110055A02Rik; Myohd1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG206167 representing BC007156
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGTTTCCTGCCAACCCTGAAGAGAAGACCCAGGAGGAGCTGTCTGGCCAGAGCCGGGCTCCTGCAT
TGACTGTGGTGTCCAAGTTCAAGGCCTCACTGGAACAGCTCCTGCAGGTCCTACATAATACAACACCCCA
CTACATTCGCTGCATCAAGCCCAACAGCCAGAGTCAGCCACAGACTTTCCTCCAAGAAGAGGTCCTGAAC
CAGTTAGAGGCCTGTGGCCTTGTGGAAACCATTCACATCAGTGCTGCTGGCTTTCCCATCCGGGTCTCTC
ACCAGAACTTCATAGAAAGATATAAACTACTGAGAAGGCTCGGACCTCGCATGTCCTCTGGCCTCGGGGG
CCTGGAGCCTGCCGAAGGGTCCTCTGAGCAGCCTCTGTGTGCCAAGGAGGCCACACTGCAACCTCTACTA
CAGGACATTCTCCATGCTCTGCCAGCTTTAATTCAGACAGCAGCCACTCCCAGTGACCCGGCTAAGAACA
CACAAATCCCACTGTACTGTGGCAGGACAAAGATCTTCATGACTGACTCCATGCTAGAGCTTCTGGAATG
TGGGCGTGCCCAGATGCTGGAGCAGTGTGCCCGTTGCATCCAGTGTGGCTGGAGGCGACACCGGCTCCAA
AAGCAGGAGAAACAGAGGCGGGCTGCCGTGCTCATCCAGGCTGCTTTTAGGTCCTGGTTAACTCGGAAAC
ACATCAGAAGGTTACACATAGCTGCCACGGTCATCAAGCACGCATGGCATAAGTGGAGAATCAGAATGGC
CTGTCTTGCCTCTAAAGAACTGGATGGTATGGAGGAGAAACCCATGCCTCAGGCTCCTGGTACCCTGCGG
TCCTCGATGTCCCCAGCACACACTAGGTTCCTGGGAGCAATAATCCATCTCTGGCCCCTGGGACTGGTGC
TAGCCAACTCAGCTGATGGCGTACGTGGCTTTCAGAGGAAACTGGTAGCCCATGCCTGCCTTCGGCTTCC
CTCAGACAGACCCAGCAACAAAGTCCAGACACCACAACAGGATCAGGCTGGTATTACATCTATCAGAGCA
CTGCCCCAGGGCTCGATAAAGTTTCACTGCAGGAAGTCTCCACTTCAGTATGCTGACATCTGCCCTGACC
CTTCAGCCTCCTGTGTTACTGGCTTTAATCAGATTCTGCTAGAAAGCCACAGGCCAGTCCAAGTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG206167 representing BC007156
Red=Cloning site Green=Tags(s)

MLFPANPEEKTQEELSGQSRAPALTVVSKFKASLEQLLQVLHNTTPHYIRCIKPNSQSQPQTFLQEEVLN
QLEACGLVETIHISAAGFPIRVSHQNFIERYKLLRRLGPRMSSGLGGLEPAEGSSEQPLCAKEATLQPLL
QDILHALPALIQTAATPSDPAKNTQIPLYCGRTKIFMTDSMLELLECGRAQMLEQCARCIQCGWRRHRLQ
KQEKQRRAAVLIQAAFRSWLTRKHIRRLHIAATVIKHAWHKWRIRMACLASKELDGMEEKPMPQAPGTLR
SSMSPAHTRFLGAIIHLWPLGLVLANSADGVRGFQRKLVAHACLRLPSDRPSNKVQTPQQDQAGITSIRA
LPQGSIKFHCRKSPLQYADICPDPSASCVTGFNQILLESHRPVQV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC007156
ORF Size 1185 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC007156, AAH07156
RefSeq Size 2856 bp
RefSeq ORF 1187 bp
Locus ID 66196
Cytogenetics 11 C
Summary Actin-based motor molecule with ATPase activity that localizes to the mitochondrion outer membrane (PubMed:24825904). Motor protein that moves towards the plus-end of actin filaments (PubMed:24825904). Required for mitochondrial inheritance during mitosis (By similarity). May be involved in mitochondrial transport or positioning (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC007156" in other vectors (4)
SKU Description Size Price
MC200885 Myohd1 (untagged) - Mouse myosin head domain containing 1 (cDNA clone MGC:7331 IMAGE:3486558), (10ug) 10 ug
$457.00
MR206167 Myohd1 (Myc-DDK-tagged) - Mouse myosin head domain containing 1 (cDNA clone MGC:7331 IMAGE:3486558) 10 ug
$289.00 $457.00
MR206167L3 Lenti ORF clone of Myohd1 (Myc-DDK-tagged) - Mouse myosin head domain containing 1 (cDNA clone MGC:7331 IMAGE:3486558) 10 ug
$757.00
MR206167L4 Lenti ORF clone of Myohd1 (mGFP-tagged) - Mouse myosin head domain containing 1 (cDNA clone MGC:7331 IMAGE:3486558) 10 ug
$757.00

Plasmid Map for MG206167

Download sequence for

Cloning scheme for MG206167

Circular map for MG206167

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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