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Map2k1 (NM_008927) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG206149
Map2k1 (tGFP-tagged) - Mouse mitogen activated protein kinase kinase 1 (Map2k1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Map2k1
Synonyms MAPKK1; Mek1; MEKK1; Prkmk1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG206149 representing NM_008927
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCAAGAAGAAGCCGACGCCCATCCAGCTGAACCCGGCCCCCGATGGCTCGGCGGTTAACGGGACCA
GCTCGGCCGAGACCAACCTGGAGGCCTTGCAGAAGAAGCTGGAGGAGCTGGAGCTTGACGAGCAGCAGCG
GAAGCGGCTCGAGGCCTTTCTGACGCAGAAGCAGAAGGTGGGGGAACTGAAGGATGATGACTTTGAGAAG
ATCAGCGAACTGGGAGCTGGCAACGGTGGAGTGGTCTTCAAGGTCTCCCACAAGCCATCTGGCCTGGTTA
TGGCTAGAAAGCTGATCCACCTGGAGATCAAACCCGCAATCCGGAACCAGATCATCCGGGAGCTGCAGGT
ACTGCACGAGTGCAACTCCCCGTACATCGTGGGCTTCTACGGGGCCTTCTACAGCGACGGCGAGATCAGC
ATCTGCATGGAGCACATGGATGGTGGGTCCTTGGATCAAGTTCTGAAGAAAGCTGGAAGAATTCCTGAGC
AAATTTTAGGAAAAGTTAGCATTGCTGTGATAAAAGGCCTGACCTATCTTCGGGAGAAGCACAAGATTAT
GCACAGAGATGTCAAGCCATCCAACATTCTAGTGAACTCACGTGGGGAGATCAAACTCTGTGATTTTGGG
GTCAGCGGGCAGCTAATTGACTCTATGGCCAACTCCTTCGTGGGCACGAGATCCTACATGTCGCCTGAGA
GACTCCAGGGGACTCACTACTCTGTGCAGTCGGACATCTGGAGCATGGGGCTCTCTCTGGTGGAGATGGC
AGTTGGGAGATACCCCATTCCTCCTCCTGATGCCAAGGAGCTGGAGCTACTGTTTGGATGCCATGTGGAA
GGAGACGCAGCCGAAACACCACCCAGGCCAAGGACCCCTGGGAGGCCTCTCAGCTCATATGGAATGGACA
GCCGACCTCCCATGGCAATTTTTGAGTTGTTGGATTACATTGTCAATGAGCCTCCTCCAAAACTGCCCAG
TGGAGTATTCAGTCTGGAGTTTCAGGATTTTGTGAATAAATGCTTAATAAAGAACCCTGCAGAGAGAGCA
GATCTGAAGCAGCTCATGGTACATGCTTTCATCAAAAGATCTGACGCCGAGGAGGTAGACTTCGCAGGCT
GGCTCTGCTCCACCATTGGGCTTAACCAGCCCAGCACACCAACCCACGCTGCCAGCATC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG206149 representing NM_008927
Red=Cloning site Green=Tags(s)

MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFEK
ISELGAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEIS
ICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFG
VSGQLIDSMANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELLFGCHVE
GDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEFQDFVNKCLIKNPAERA
DLKQLMVHAFIKRSDAEEVDFAGWLCSTIGLNQPSTPTHAASI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008927
ORF Size 1179 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008927.4
RefSeq Size 2388 bp
RefSeq ORF 1182 bp
Locus ID 26395
UniProt ID P31938
Cytogenetics 9 34.55 cM
Summary Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.UniProtKB/Swiss-Prot Function
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"NM_008927" in other vectors (4)
SKU Description Size Price
MC202574 Map2k1 (untagged) - Mouse mitogen-activated protein kinase kinase 1 (Map2k1), (10ug) 10 ug
$457.00
MR206149 Map2k1 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase 1 (Map2k1) 10 ug
$289.00 $457.00
MR206149L3 Lenti ORF clone of Map2k1 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase 1 (Map2k1) 10 ug
$757.00
MR206149L4 Lenti ORF clone of Map2k1 (mGFP-tagged) - Mouse mitogen-activated protein kinase kinase 1 (Map2k1) 10 ug
$757.00

Plasmid Map for MG206149

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG206149
Circular map for MG206149

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