Metap1 (NM_175224) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG206041
Metap1 (tGFP-tagged) - Mouse methionyl aminopeptidase 1 (Metap1)
  $657.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Metap1
Synonyms 1700029C17Rik; AW047992; mKIAA0094
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG206041 representing NM_175224
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGGTGGAGACGCGGGTGTGCGAGACGGACGGCTGCAGCAGCGAGGCCAAGCTCCAGTGTCCCA
CCTGCATCAAGCTGGGCATCCAGGGCTCGTACTTCTGCTCGCAGGAATGCTTTAAAGGAAGCTGGGCCAC
TCACAAGTTACTACATAAGAAAGCAAAAGATGAAAAGGCAAAGCGAGAAGTGTGTTCCTGGACTGTAGAA
GGTGATGTCAACACTGACCCATGGGCAGGCTATCGGTACACTGGTAAACTCAGGCCACATTATCCACTGA
TGCCAACACGGCCAGTGCCCAGTTACATTCAGAGGCCGGACTATGCTGACCATCCCTTAGGAATGTCTGA
ATCGGAGCAGGCTCTTAAAGGTACCTCTCAGATTAAGTTACTCTCATCTGAAGATATAGAAGGGATGCGA
CTCGTGTGTAGGCTTGCCAGAGAAGTTTTGGACATTGCTGCTGGGATGATTAAAGCGGGTGTAACTACTG
AAGAGATCGACCACGCTGTACACTTAGCATGCATTGCAAGAAATTGCTATCCTTCTCCCCTGAATTACTA
TAACTTCCCAAAGTCTTGTTGTACCTCAGTGAATGAAGTCATCTGCCATGGGATTCCAGACAGGCGGCCT
TTGCAAGAAGGTGATATTGTTAATGTGGACATCACTCTTTATCGAAATGGTTACCATGGGGATCTAAATG
AGACCTTTTTCGTTGGCGATGTGGATGAAGGAGCACGGAAACTTGTTCAGACTACATATGAGTGCCTGAT
GCAAGCCATCGATGCAGTGAAGCCTGGTGTTCGATACAGAGAACTGGGAAACATCATTCAGAAACACGCC
CAAGCAAATGGATTCTCAGTTGTTCGAAGCTATTGTGGGCATGGGATCCACAAACTTTTCCATACAGCCC
CCAATGTCCCGCACTATGCCAAAAATAAAGCAGTTGGTGTGATGAAGTCGGGTCATGTGTTTACAATTGA
GCCAATGATTTGTGAAGGTGGATGGCAGGATGAGACTTGGCCAGATGGCTGGACTGCAGTGACGAGGGAC
GGGAAGCGATCTGCTCAGTTTGAGCACACCCTGCTGGTCACGGACACTGGCTGTGAAATTCTCACCCGGA
GACTCGATAGCTCTCGGCCTCATTTCATGTCCCAGTTT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG206041 representing NM_175224
Red=Cloning site Green=Tags(s)

MAAVETRVCETDGCSSEAKLQCPTCIKLGIQGSYFCSQECFKGSWATHKLLHKKAKDEKAKREVCSWTVE
GDVNTDPWAGYRYTGKLRPHYPLMPTRPVPSYIQRPDYADHPLGMSESEQALKGTSQIKLLSSEDIEGMR
LVCRLAREVLDIAAGMIKAGVTTEEIDHAVHLACIARNCYPSPLNYYNFPKSCCTSVNEVICHGIPDRRP
LQEGDIVNVDITLYRNGYHGDLNETFFVGDVDEGARKLVQTTYECLMQAIDAVKPGVRYRELGNIIQKHA
QANGFSVVRSYCGHGIHKLFHTAPNVPHYAKNKAVGVMKSGHVFTIEPMICEGGWQDETWPDGWTAVTRD
GKRSAQFEHTLLVTDTGCEILTRRLDSSRPHFMSQF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175224
ORF Size 1158 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175224.4, NP_780433.1
RefSeq Size 2550 bp
RefSeq ORF 1161 bp
Locus ID 75624
UniProt ID Q8BP48
Cytogenetics 3 G3
Summary Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_175224" in other vectors (4)
SKU Description Size Price
MC211673 Metap1 (untagged) - Mouse methionyl aminopeptidase 1 (Metap1), (10ug) 10 ug
$503.00
MR206041 Metap1 (Myc-DDK-tagged) - Mouse methionyl aminopeptidase 1 (Metap1) 10 ug
$289.00 $457.00
MR206041L3 Lenti ORF clone of Metap1 (Myc-DDK-tagged) - Mouse methionyl aminopeptidase 1 (Metap1) 10 ug
$757.00
MR206041L4 Lenti ORF clone of Metap1 (mGFP-tagged) - Mouse methionyl aminopeptidase 1 (Metap1) 10 ug
$757.00

Plasmid Map for MG206041

Download sequence for

Cloning scheme for MG206041

Circular map for MG206041

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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