Metap1 (NM_175224) Mouse Untagged Clone

SKU
MC211673
Metap1 (untagged) - Mouse methionyl aminopeptidase 1 (Metap1), (10ug)
  $503.00
4 Weeks*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Metap1
Synonyms 1700029C17Rik; AW047992; mKIAA0094
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC211673 representing NM_175224
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGGTGGAGACGCGGGTGTGCGAGACGGACGGCTGCAGCAGCGAGGCCAAGCTCCAGTGTCCCA
CCTGCATCAAGCTGGGCATCCAGGGCTCGTACTTCTGCTCGCAGGAATGCTTTAAAGGAAGCTGGGCCAC
TCACAAGTTACTACATAAGAAAGCAAAAGATGAAAAGGCAAAGCGAGAAGTGTGTTCCTGGACTGTAGAA
GGTGATGTCAACACTGACCCATGGGCAGGCTATCGGTACACTGGTAAACTCAGGCCACATTATCCACTGA
TGCCAACACGGCCAGTGCCCAGTTACATTCAGAGGCCGGACTATGCTGACCATCCCTTAGGAATGTCTGA
ATCGGAGCAGGCTCTTAAAGGTACCTCTCAGATTAAGTTACTCTCATCTGAAGATATAGAAGGGATGCGA
CTCGTGTGTAGGCTTGCCAGAGAAGTTTTGGACATTGCTGCTGGGATGATTAAAGCGGGTGTAACTACTG
AAGAGATCGACCACGCTGTACACTTAGCATGCATTGCAAGAAATTGCTATCCTTCTCCCCTGAATTACTA
TAACTTCCCAAAGTCTTGTTGTACCTCAGTGAATGAAGTCATCTGCCATGGGATTCCAGACAGGCGGCCT
TTGCAAGAAGGTGATATTGTTAATGTGGACATCACTCTTTATCGAAATGGTTACCATGGGGATCTAAATG
AGACCTTTTTCGTTGGCGATGTGGATGAAGGAGCACGGAAACTTGTTCAGACTACATATGAGTGCCTGAT
GCAAGCCATCGATGCAGTGAAGCCTGGTGTTCGATACAGAGAACTGGGAAACATCATTCAGAAACACGCC
CAAGCAAATGGATTCTCAGTTGTTCGAAGCTATTGTGGGCATGGGATCCACAAACTTTTCCATACAGCCC
CCAATGTCCCGCACTATGCCAAAAATAAAGCAGTTGGTGTGATGAAGTCGGGTCATGTGTTTACAATTGA
GCCAATGATTTGTGAAGGTGGATGGCAGGATGAGACTTGGCCAGATGGCTGGACTGCAGTGACGAGGGAC
GGGAAGCGATCTGCTCAGTTTGAGCACACCCTGCTGGTCACGGACACTGGCTGTGAAATTCTCACCCGGA
GACTCGATAGCTCTCGGCCTCATTTCATGTCCCAGTTTTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_175224
Insert Size 1161 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175224.4, NP_780433.1
RefSeq Size 2549 bp
RefSeq ORF 1161 bp
Locus ID 75624
UniProt ID Q8BP48
Cytogenetics 3 G3
Summary Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_175224" in other vectors (4)
SKU Description Size Price
MG206041 Metap1 (tGFP-tagged) - Mouse methionyl aminopeptidase 1 (Metap1) 10 ug
$657.00
MR206041 Metap1 (Myc-DDK-tagged) - Mouse methionyl aminopeptidase 1 (Metap1) 10 ug
$289.00 $457.00
MR206041L3 Lenti ORF clone of Metap1 (Myc-DDK-tagged) - Mouse methionyl aminopeptidase 1 (Metap1) 10 ug
$757.00
MR206041L4 Lenti ORF clone of Metap1 (mGFP-tagged) - Mouse methionyl aminopeptidase 1 (Metap1) 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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