Psmd4 (BC009005) Mouse Tagged ORF Clone

SKU MG205907

Psmd4 (tGFP-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 4 (cDNA clone MGC:6683 IMAGE:3581937), complete

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Psmd4
• Synonyms: Mcb1, angiocidin
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG205907 representing BC009005; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGTGTTGGAGAGCACTATGGTTTGTGTGGACAACAGTGAGTACATGCGGAACGGAGACTTCCTTCCCA; CCCGGCTGCAGGCCCAGCAGGATGCCGTCAACATTGTATGTCACTCAAAGACCCGAAGCAACCCTGAGAA; TAACGTGGGCCTGATCACACTGGCCAATGACTGTGAGGTGCTGACCACACTCACCCCGGACACTGGCCGT; ATCCTCTCCAAGCTCCACACTGTCCAACCCAAAGGCAAGATCACCTTCTGCACTGGCATCCGCGTGGCCC; ACTTGGCTCTGAAGCACCGGCAGGGCAAGAATCACAAGATGCGCATCATCGCCTTTGTCGGTAGCCCTGT; GGAGGACAACGAGAAGGATCTGGTGAAACTAGCTAAACGCCTTAAGAAAGAAAAAGTGAATGTTGACATC; ATTAATTTTGGGGAAGAGGAGGTGAACACAGAGAAGCTGACAGCCTTTGTGAACACACTGAATGGCAAGG; ATGGAACTGGGTCCCATCTAGTGACAGTGCCTCCTGGACCTAGCTTGGCTGATGCTCTCATCAGTTCTCC; TATTCTGGCTGGTGAAGGCGGTGCCATGCTGGGTCTTGGTGCCAGTGACTTTGAGTTTGGAGTAGATCCC; AGTGCTGATCCTGAATTGGCCCTGGCCCTTCGAGTCTCTATGGAAGAGCAGCGGCAGCGGCAGGAGGAAG; AGGCACGGCGGGCCGCTGCGGCCTCTGCAGCTGAGGCTGGAATTGCTACACCTGGGACTGAAGGTGAAAG; AGACTCGGATGACGCCCTACTGAAGATGACCATCAACCAGCAGGAGTTTGGCCGTCCTGGGCTTCCAGAC; CTAAGCAGCATGACTGAGGAAGAGCAGATCGCCTACGCCATGCAGATGTCCCTGCAGGGAACAGAGTTTA; GCCAAGAATCGGCTGACATGGATGCCAGCTCAGCCATGGACACATCTGATCCAGTCAAGGAGGAGGATGA; CTATGACGTCATGCAGGACCCGGAGTTCCTTCAGAGCGTCCTAGAGAACCTTCCAGGTGTGGATCCCAAC; AATGCAGCCATTCGAAGTGTCATGGGGGCTCTGGCCTCCCAGGCCACCAAGGATGGCAAGAATGACAAGA; AAGAGGAAGAGAAGAAG; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG205907 representing BC009005; Red=Cloning site Green=Tags(s); ; MVLESTMVCVDNSEYMRNGDFLPTRLQAQQDAVNIVCHSKTRSNPENNVGLITLANDCEVLTTLTPDTGR; ILSKLHTVQPKGKITFCTGIRVAHLALKHRQGKNHKMRIIAFVGSPVEDNEKDLVKLAKRLKKEKVNVDI; INFGEEEVNTEKLTAFVNTLNGKDGTGSHLVTVPPGPSLADALISSPILAGEGGAMLGLGASDFEFGVDP; SADPELALALRVSMEEQRQRQEEEARRAAAASAAEAGIATPGTEGERDSDDALLKMTINQQEFGRPGLPD; LSSMTEEEQIAYAMQMSLQGTEFSQESADMDASSAMDTSDPVKEEDDYDVMQDPEFLQSVLENLPGVDPN; NAAIRSVMGALASQATKDGKNDKKEEEKK; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC009005
• ORF Size: 1137 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC009005, AAH09005
• RefSeq Size: 1301 bp
• RefSeq ORF: 1139 bp
• Locus ID: 19185
• Cytogenetics: 3 40.74 cM
• Summary: Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMD4 acts as an ubiquitin receptor subunit through ubiquitin-interacting motifs and selects ubiquitin-conjugates for destruction. Displays a preferred selectivity for longer polyubiquitin chains.[UniProtKB/Swiss-Prot Function]