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Fcgrt (BC003786) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG205688
Fcgrt (tGFP-tagged) - Mouse Fc receptor, IgG, alpha chain transporter (cDNA clone MGC:6014 IMAGE:3485649)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
$489.00 $657.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fcgrt
Synonyms FcRn
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG205688 representing BC003786
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGATGCCACTGCCCTGGGCCCTCAGCCTCTTGTTGGTCCTCCTGCCTCAGACCTGGGGCTCAGAGA
CCCGCCCCCCACTGATGTATCATCTCACGGCTGTGTCAAACCCATCTACGGGGCTTCCCTCTTTCTGGGC
CACAGGCTGGTTGGGTCCTCAGCAGTATCTGACCTACAACAGCCTGCGGCAGGAAGCTGACCCCTGTGGG
GCCTGGATGTGGGAAAATCAGGTGTCTTGGTATTGGGAGAAGGAGACCACAGACCTCAAAAGCAAAGAAC
AGCTCTTCTTGGAGGCCCTCAAGACCCTGGAGAAGATATTAAATGGTCAGAAGAGGGGGACCTACACACT
GCAGGGCCTGCTGGGCTGTGAACTGGCCTCGGATAATTCCTCAGTGCCCACGGCTGTGTTTGCCCTCAAT
GGTGAGGAGTTTATGAAATTCAACCCAAGAATCGGCAATTGGACTGGGGAGTGGCCTGAGACGGAAATCG
TTGCTAATCTGTGGATGAAGCAGCCTGATGCGGCCAGGAAGGAGAGCGAGTTCCTGCTAAACTCTTGTCC
GGAGCGACTGCTAGGCCACCTGGAGAGGGGCCGACGGAACCTGGAGTGGAAGGAGCCGCCGTCTATGCGC
CTGAAGGCCCGTCCTGGCAACTCTGGCTCCTCCGTGCTGACCTGTGCTGCTTTCTCCTTCTACCCACCGG
AGCTCAAGTTCCGATTCCTGCGCAATGGGCTAGCCTCAGGCTCCGGGAATTGCAGCACTGGTCCCAATGG
AGATGGCTCTTTCCACGCATGGTCATTGCTGGAGGTCAAACGTGGAGATGAGCACCATTATCAATGTCAA
GTGGAGCATGAGGGGCTGGCACAGCCTCTCACTGTGGACCTAGATTCATCAGCCAGATCTTCTGTGCCTG
TGGTTGGAATCGTTCTTGGTTTATTGCTGGTGGTAGTGGCCATCGCAGGCGGTGTGCTGCTGTGGGGCAG
GATGCGCAGCGGTCTGCCAGCCCCATGGCTTTCTCTCAGCGGCGATGACTCTGGTGACCTGTTGCCTGGT
GGGAACTTGCCCCCAGAAGCTGAACCTCAAGGTGCAAATGCCTTTCCAGCCACTTCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG205688 representing BC003786
Red=Cloning site Green=Tags(s)

MGMPLPWALSLLLVLLPQTWGSETRPPLMYHLTAVSNPSTGLPSFWATGWLGPQQYLTYNSLRQEADPCG
AWMWENQVSWYWEKETTDLKSKEQLFLEALKTLEKILNGQKRGTYTLQGLLGCELASDNSSVPTAVFALN
GEEFMKFNPRIGNWTGEWPETEIVANLWMKQPDAARKESEFLLNSCPERLLGHLERGRRNLEWKEPPSMR
LKARPGNSGSSVLTCAAFSFYPPELKFRFLRNGLASGSGNCSTGPNGDGSFHAWSLLEVKRGDEHHYQCQ
VEHEGLAQPLTVDLDSSARSSVPVVGIVLGLLLVVVAIAGGVLLWGRMRSGLPAPWLSLSGDDSGDLLPG
GNLPPEAEPQGANAFPATS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC003786
ORF Size 1107 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC003786, AAH03786
RefSeq Size 1617 bp
RefSeq ORF 1109 bp
Locus ID 14132
Cytogenetics 7 29.12 cM
Summary Binds to the Fc region of monomeric immunoglobulins gamma (PubMed:7504013). Mediates the selective uptake of IgG from milk and helps newborn animals to acquire passive immunity. IgG in the milk is bound at the apical surface of the intestinal epithelium. The resultant FcRn-IgG complexes are transcytosed across the intestinal epithelium and IgG is released from FcRn into blood or tissue fluids (By similarity). Possible role in transfer of immunoglobulin G from mother to fetus (By similarity).UniProtKB/Swiss-Prot Function
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"BC003786" in other vectors (4)
SKU Description Size Price
MC200332 Fcgrt (untagged) - Mouse Fc receptor, IgG, alpha chain transporter (cDNA clone MGC:6014 IMAGE:3485649), (10ug) 10 ug
$457.00
MR205688 Fcgrt (Myc-DDK-tagged) - Mouse Fc receptor, IgG, alpha chain transporter (cDNA clone MGC:6014 IMAGE:3485649) 10 ug
$289.00 $457.00
MR205688L3 Lenti ORF clone of Fcgrt (Myc-DDK-tagged) - Mouse Fc receptor, IgG, alpha chain transporter (cDNA clone MGC:6014 IMAGE:3485649) 10 ug
$757.00
MR205688L4 Lenti ORF clone of Fcgrt (mGFP-tagged) - Mouse Fc receptor, IgG, alpha chain transporter (cDNA clone MGC:6014 IMAGE:3485649) 10 ug
$757.00

Plasmid Map for MG205688

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG205688
Circular map for MG205688

file-alt Citations

A Novel Tetravalent CD95/Fas Fusion Protein With Superior CD95L/FasL Antagonism
Lang, Paulus, Zaitseva et al
Proteins (2024)
Cited by 2
AI Summary: OriGene contributed to this study by providing expression plasmids for human FcRn (#RC200364) and murine FcRn (#MG205688), which were used to investigate the binding properties of the engineered CD95 fusion proteins.
Snippet: Expression plasmids encoding for the following proteins were obtained from Origene (Rockville, Maryland): Human FcRn (#RC200364), murine FcRn (MG#205688), and
Showing 1-1 of 1 papers.
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