Dcdc2a (NM_177577) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG205677
Dcdc2a (tGFP-tagged) - Mouse doublecortin domain containing 2a (Dcdc2a)
  $657.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dcdc2a
Synonyms AW492955; Dcdc2; RU; RU2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG205677 representing NM_177577
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACGGTCCCAGCTCCAGGTCCAGCCACCTGTCGCAGCCGGTGGTGAAGAGTGTGCTGGTGTACCGCA
ATGGGGACCCCTTTTTCGCCGGGCGGCGTGTCGTCATTCACGAGAAGAAGGTGTCCAGCTTCGATGTCTT
CCTCAAAGAAGTGACTGGCGGTGTGCAGGCGCCTTTTGGGGCGGTTAGGAACATCTACACCCCGAGGACG
GGCCACCGCATCCGAAAACTGGACCAGATAGAGAGCGGGGGCAACTATGTGGCTGGGGGCCCGGAAGCGT
TCAAGAAACTCAATTACTTGGACATAGGCGAAATCAAGAAAAGACCAATGGAAGCTGTGAATACCGAGGT
GAAACCTGTCATTCACAGCAGGATCAACGTGTCCGCCCGCTTCAGGAAGTCGCTGCACGAGCCCTGCACC
ATTTTCTTGATTGCAAATGGAGACCTCATAAGCCCGGCTTCTCGCCTCCTCATCCCTAAAAAAGCTTTAA
ATCAGTGGGATCATGTACTACAAATGGTCACAGAAAAGATAACTCTCCGGAGTGGGGCTGTCCACAGGCT
ATATACGTTAGAAGGAAAGCTGGTTGAGAGTGGAGCAGAACTGGAGAATGGGCAGTTCTATGTGGCCGTC
GGCAGAGATAAGTTTAAGAGACTACCGTACAGTGAGTTACTTTTTGACAAGTCAGCAATGAGAAGGCCTT
ACGGTCAGAAAGCGTCTTCACTACCTCCCATGGTTGGATCCAGGAAGTCTAAAGGGAGCGGAAATTATCG
GCAATCCAAGTCAACCATCGGGTCTAGTGATAACTCATCTCCTCAGCCTCTGAAAAGGAAAGGGAAGAAA
GACTCAAACTCAGAAAAACCCACGAAAGTGAAACAGAGTGTGAAGTCGAAGACTTCCCACCAAGCAATCC
CAGACAATGGTGAAGGCATTTTCAAAGCTGGAGCAGAGAGATCTGAAACAAGGGGGGCAGCAGAAGTTCA
AGAAGATGAAGACACCCAGGTTGAGGTTCCAGTGGACCAGAGGCCAGCAGAAATAGTTGATGAGGAAGAA
GATGGTGAGAAAACAAGCAAAGATGCCAACCAGAAGGAAGACTTTTCAGCCATGAATGGTGAGACTGAAG
ACAGAGGGGGGAGCAAAGCGGCTGGTACCTCTGAGCAGGAGGAGGGAATCCCAGACCACGGGGAGAAGAA
GGCAAGCCCCTCTCGGGTAAATGGTGGCACGGATGAAGAGAATGGGGAGGAACTGGACCAGGTTGCCGAG
GAGCTCCAATCAACAGAGGACGAGAAAGGAAAGGCTGAAGGAGACAACAGTGGGCAAGATGAGGCTGGTT
TGGATGCTCAAAGACCACCAAGACCAGAAGTGACAGTCACCAGCCCTCAAGAGAATGAAGAGAACGAAGC
AAACAAGGCCTCTTCTGCTGTGGCA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG205677 representing NM_177577
Red=Cloning site Green=Tags(s)

MNGPSSRSSHLSQPVVKSVLVYRNGDPFFAGRRVVIHEKKVSSFDVFLKEVTGGVQAPFGAVRNIYTPRT
GHRIRKLDQIESGGNYVAGGPEAFKKLNYLDIGEIKKRPMEAVNTEVKPVIHSRINVSARFRKSLHEPCT
IFLIANGDLISPASRLLIPKKALNQWDHVLQMVTEKITLRSGAVHRLYTLEGKLVESGAELENGQFYVAV
GRDKFKRLPYSELLFDKSAMRRPYGQKASSLPPMVGSRKSKGSGNYRQSKSTIGSSDNSSPQPLKRKGKK
DSNSEKPTKVKQSVKSKTSHQAIPDNGEGIFKAGAERSETRGAAEVQEDEDTQVEVPVDQRPAEIVDEEE
DGEKTSKDANQKEDFSAMNGETEDRGGSKAAGTSEQEEGIPDHGEKKASPSRVNGGTDEENGEELDQVAE
ELQSTEDEKGKAEGDNSGQDEAGLDAQRPPRPEVTVTSPQENEENEANKASSAVA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177577
ORF Size 1425 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177577.3, NP_808245.2
RefSeq Size 2370 bp
RefSeq ORF 1428 bp
Locus ID 195208
UniProt ID Q5DU00
Cytogenetics 13 A3.1
Summary This gene encodes a member of the doublecortin family. The protein encoded by this gene contains two doublecortin domains. The doublecortin domain has been demonstrated to bind tubulin and enhance microtubule polymerization. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. provided by RefSeq, Sep 2010
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Documents
Resources
"NM_177577" in other vectors (5)
SKU Description Size Price
MC203097 Dcdc2a (untagged) - Mouse doublecortin domain containing 2a (Dcdc2a), transcript variant 1, (10ug) 10 ug
$457.00
MG226943 Dcdc2a (tGFP-tagged) - Mouse doublecortin domain containing 2a (Dcdc2a) transcript variant 1, (10ug) 10 ug
$703.00
MR226943 Dcdc2a (Myc-DDK-tagged) - Mouse doublecortin domain containing 2a (Dcdc2a), transcript variant 1 10 ug
$503.00
MR226943L3 Lenti ORF clone of Dcdc2a (Myc-DDK-tagged) - Mouse doublecortin domain containing 2a (Dcdc2a), transcript variant 1 10 ug
$803.00
MR226943L4 Lenti ORF clone of Dcdc2a (mGFP-tagged) - Mouse doublecortin domain containing 2a (Dcdc2a), transcript variant 1 10 ug
$803.00

Plasmid Map for MG205677

Download sequence for

Cloning scheme for MG205677

Circular map for MG205677

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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